Browse > Article
http://dx.doi.org/10.3347/kjp.2017.55.2.109

Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii  

Moon, Eun-Kyung (Department of Medical Zoology, Kyung Hee University School of Medicine)
Kong, Hyun-Hee (Department of Parasitology, Dong-A University College of Medicine)
Hong, Yeonchul (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine)
Lee, Hae-Ahm (Department of Pharmacology, Kyungpook National University School of Medicine)
Quan, Fu-Shi (Department of Medical Zoology, Kyung Hee University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.55, no.2, 2017 , pp. 109-114 More about this Journal
Abstract
Protein arginine methyltransferase (PRMT) is an important epigenetic regulator in eukaryotic cells. During encystation, an essential process for Acanthamoeba survival, the expression of a lot of genes involved in the encystation process has to be regulated in order to be induced or inhibited. However, the regulation mechanism of these genes is yet unknown. In this study, the full-length 1,059 bp cDNA sequence of Acanthamoeba castellanii PRMT1 (AcPRMT1) was cloned for the first time. The AcPRMT1 protein comprised of 352 amino acids with a SAM-dependent methyltransferase PRMT-type domain. The expression level of AcPRMT1 was highly increased during encystation of A. castellanii. The EGFP-AcPRMT1 fusion protein was distributed over the cytoplasm, but it was mainly localized in the nucleus of Acanthamoeba. Knock down of AcPRMT1 by synthetic siRNA with a complementary sequence failed to form mature cysts. These findings suggested that AcPRMT1 plays a critical role in the regulation of encystation of A. castellanii. The target gene of AcPRMT1 regulation and the detailed mechanisms need to be investigated by further studies.
Keywords
Acanthamoeba castellanii; characterization; encystation; protein arginine methyltransferase 1;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 2003; 16: 273-307.   DOI
2 Moon EK, Xuan YH, Chung DI, Hong Y, Kong HH. Microarray analysis of differentially expressed genes between cysts and trophozoites of Acanthamoeba castellanii. Korean J Parasitol 2011; 49: 341-347.   DOI
3 Portela A, Esteller M. Epigenetic modifications and human disease. Nat Biotechnol 2010; 28: 1057-1068.   DOI
4 Kirmizis A, Santos-Rosa H, Penkett CJ, Singer MA, Vermeulen M, Mann M, Bahler J, Green RD, Kouzarides T. Arginine methylation at histone H3R2 controls deposition of H3K4 trimethylation. Nature 2007; 449: 928-932.   DOI
5 Bedford MT, Clarke SG. Protein arginine methylation in mammals: who, what, and why. Mol Cell 2009; 33: 1-13.   DOI
6 Pahlich S, Zakaryan RP, Gehring H. Protein arginine methylation: cellular functions and methods of analysis. Biochim Biophys Acta 2006; 1764: 1890-1903.   DOI
7 Niewmierzycka A, Clarke S. S-adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase. J Biol Chem 1999; 274: 814-824.   DOI
8 Pawlak MR, Scherer CA, Chen J, Roshon MJ, Ruley HE. Arginine N-methyltransferase 1 is required for early postimplantation mouse development, but cells deficient in the enzyme are viable. Mol Cell Biol 2000; 20: 4859-4869.   DOI
9 Krause CD, Yang ZH, Kim YS, Lee JH, Cook JR, Pestka S. Protein arginine methyltransferases: evolution and assessment of their pharmacological and therapeutic potential. Pharmacol Ther 2007; 113: 50-87.   DOI
10 Katsanis N, Yaspo ML, Fisher EM. Identification and mapping of a novel human gene, HRMT1L1, homologous to the rat protein arginine N-methyltransferase 1 (PRMT1) gene. Mamm Genome 1997; 8: 526-529.   DOI
11 Nicholson TB, Chen T, Richard S. The physiological and pathophysiological role of PRMT1-mediated protein arginine methylation. Pharmacol Res 2009; 60: 466-474.   DOI
12 Kraus WL, Wong J. Nuclear receptor-dependent transcription with chromatin. Is it all about enzymes? Eur J Biochem 2002; 269: 2275-2283.   DOI
13 Aqeel Y, Siddiqui R, Khan NA. Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii. Parasitol Res 2013; 112: 1221-1127.   DOI
14 Fan Q, Miao J, Cui L, Cui L. Characterization of PRMT1 from Plasmodium falciparum. Biochem J 2009; 421: 107-118.   DOI
15 Borbolla-Vazquez J, Orozco E, Betanzos A, Rodriguez MA. Entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the H4 histone. Parasit Vectors 2015; 8: 219.   DOI
16 Bowers B, Korn ED. The fine structure of Acanthamoeba castellanii (Neff strain). II. Encystment. J Cell Biol 1969; 41: 786-805.   DOI
17 Moon EK, Hong Y, Chung DI, Kong HH. Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba. Mol Biochem Parasitol 2012; 185: 121-126.   DOI
18 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408.   DOI
19 Litt M, Qiu Y, Huang S. Histone arginine methylations: their roles in chromatin dynamics and transcriptional regulation. Biosci Rep 2009; 29: 131-141.   DOI
20 Moon EK, Hong Y, Chung DI, Goo YK, Kong HH. Identification of protein arginine methyltransferase 5 as a regulator for encystation of Acanthamoeba. Korean J Parasitol 2016; 54: 133-1338.   DOI
21 Di Lorenzo A, Bedford MT. Histone arginine methylation. FEBS Lett 2011; 585: 2024-2031.   DOI
22 Mansure JJ, Furtado DR, de Oliveira FM, Rumjanek FD, Franco GR, Fantappie MR. Cloning of a protein arginine methyltransferase PRMT1 homologue from Schistosoma mansoni: evidence for roles in nuclear receptor signaling and RNA metabolism. Biochem Biophys Res Commun 2005; 335: 1163-1172.   DOI
23 Wang H, Huang ZQ, Xia L, Feng Q, Erdjument-Bromage H, Strahl BD, Briggs SD, Allis CD, Wong J, Tempst P, Zhang Y. Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor. Science 2001; 293: 853-857.   DOI
24 Pal S, Vishwanath SN, Erdjument-Bromage H, Tempst P, Sif S. Human SWI/SNF-associated PRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes. Mol Cell Biol 2004; 24: 9630-9645.   DOI