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http://dx.doi.org/10.14478/ace.2022.1015

Sensitive and Selective Electrochemical Glucose Biosensor Based on a Carbon Nanotube Electronic Film  

Lee, Seung-Woo (Department of Fine Chemistry, Seoul National University of Science and Technology)
Lee, Dongwook (Department of Fine Chemistry, Seoul National University of Science and Technology)
Seo, Byeong-Gwuan (Department of Fine Chemistry, Seoul National University of Science and Technology)
Publication Information
Applied Chemistry for Engineering / v.33, no.2, 2022 , pp. 188-194 More about this Journal
Abstract
This work presents a non-destructive and straightforward approach to assemble a large-scale conductive electronic film made of a pre-treated single-walled carbon nanotube (SWCNT) solution. For effective electron transfer between the immobilized enzyme and SWCNT electronic film, we optimized the pre-treatment step of SWCNT with p-terphenyl-4,4"-dithiol and dithiothreitol. Glucose oxidase (GOx, a model enzyme in this study) was immobilized on the SWCNT electronic film following the positively charged polyelectrolyte layer deposition. The glucose detection was realized through effective electron transfer between the immobilized GOx and SWCNT electronic film at the negative potential value (-0.45 V vs. Ag/AgCl). The SWCNT electronic film-based glucose biosensor exhibited a sensitivity of 98 ㎂/mM·cm2. In addition, the SWCNT electronic film biosensor showed the excellent selectivity (less than 4 % change) against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, dopamine, and acetaminophen, by avoiding co-oxidation of the interfering substances at the negative potential value.
Keywords
Glucose biosensor; Electrochemistry; Carbon nanotube; Glucose oxidase; Electron transfer;
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Times Cited By KSCI : 2  (Citation Analysis)
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