Browse > Article
http://dx.doi.org/10.4489/KJM.2012.40.3.159

Biological Activities of Water Extract and Solvent Fractions of an Edible Mushroom, Hericium erinaceus  

Kim, Jun-Ho (Department of Fine Chemistry and New Materials, Sangji University)
Publication Information
The Korean Journal of Mycology / v.40, no.3, 2012 , pp. 159-163 More about this Journal
Abstract
This study was performed to investigate the biological activities of Hericium erinaceus fruiting body, including antioxidative, fibrinolytic, thrombin inhibitory and ${\alpha}$-glucosidase inhibitory activities. The water extract of H. erinaceus was fractionated into hexane, chloroform, ethyl acetate, butanol and water fractions, and each of these fractions was individually assayed. The water extract showed high antioxidative activity at 87.78%. The water fraction showed the highest extraction yield at 35.68% (w/w). In the fraction activity tests, butanol and water fractions showed strong antioxidative activities at 87.91% and 92.27%, respectively. Assays for fibrinolytic activity indicated that only the ethyl acetate fraction has significant efficacy at 2.96 plasmin units/mL. The 10-fold dilution of hexane fraction showed high thrombin inhibitory activity, and ${\alpha}$-glucosidase inhibitory activity at 77.67% and 90.53%, respectively. In conclusion, solvent fractions of H. erinaceus can be used as materials for the development of biofunctional foods to prevent cardiovascular diseases.
Keywords
Antioxidative activity; Fibrinolytic activity; Fruiting body; H. erinaceus; Thrombin inhibitory activity;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Mizuno, T., Wasa, T., Ito, H., Suzuki, C. and Ukai, N. 1992. Antitumor active polysaccharides isolated from the fruiting body of Hericum erinaceus: an edible and medicinal mushroom called Yamabushitake or Houtou. Biosci. Biotech. Biochem. 56:347-348.   DOI   ScienceOn
2 Oh, H. S. and Kim, J. H. 2007. Physiological functionalities of hot water extract of Codonopsis lanceolata and some medicinal materials, and their mixtures. Korean J. Community Living Sci. 18:407-415.
3 Ryu, S. R., Lee, W. Y. and Ka, K. H. 2009. Comparative study on the sawdust cultivation and the antioxidant of Hericium spp. Kor. J. Mycol. 37(1):80-85. (in Korean).   DOI   ScienceOn
4 Shin, H. H. and Choi, H. S. 1998. Purification and partial characterization of a metalloprotease in Flammulina velutipes. J. Microbiol. 36:20-25.
5 Tsukagoshi, S. and Ohashi, F. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and rat ascites hepatoma AH-13 by oral use. Gann. 65:557-558
6 Watanabe, J., Kawabata, J., Kurihara, H. and Niki, R. 1997. Isolation and identification of alpha-glucosidase inhibitors from Tochu-cha (Eucommia ulmoides). Biosci. Biotechnol. Biochem. 61:177-178.   DOI   ScienceOn
7 Doljak, B., Stegnar, M., Urleb, U., Kreft, S., Umek, A., Ciglaric, M., Strukelj, B. and Popovic, T. 2001. Screening for selective thrombin inhibitor in mushrooms. Blood Coagul. Fibrinolysis 12:123-128.   DOI   ScienceOn
8 Haverkate, F. and Traas, D. W. 1974. Dose-response curves in the fibrin plate assay. Fibrinolytic activity of protease. Thromb. Haemost. 32:356-365.
9 Hong, K. H., Kim, B. Y. and Kim, H. K. 2004. Analysis of nutritional components in Pleurotus ferulea. Kor. J. Food Sci. Technol. 36(4):563-567. (in korean).
10 Hyun, K. W., Jeong, S. C., Lee, D. H., Park, J. S. and Lee, J. S. 2006. Isolation and characterization of a noble platelet aggregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus. Peptides. 27:1173-1178.   DOI   ScienceOn
11 Kang, M. G., Bolormaa, Z., Lee, J. S., Seo, G. S. and Lee, J. S. 2011. Antihypertensive activity and anti-gout activity of mushroom Sarcodon aspratus. Kor. J. Mycol. 39(1):53-56. (in Korean).   DOI   ScienceOn
12 Kawagishi, H., Shimada, A., Hosokawa, S., Mori, H., Sakamoto, H., Ishiguro, Y., Sakemi, S., Bordner, J., Kojima, N. and Furukawa, S. 1996. Erinacines E, F and G stimulator of nerve growth factor(NGF)-synthesis from the mycelia of Hericum erinaceus. Tetrahedron Letters. 37:7399-7402.   DOI   ScienceOn
13 Kim, J. H. and Kim, Y. S. 2001. Characterization of a metalloenzyme from a wild mushroom, Tricholoma saponaceum. Biosci. Biotech. Biochem. 65(2):356-362.   DOI   ScienceOn
14 Kim, Y. J., Kim, C. K. and Kwon, Y. J. 1997. Isolation of antioxidative components of Perillae semen. Kor. J. Food Sci. Technol. 29(1):38-43.
15 Choi, J. H., Ha, T. M., Kim, Y. H. and Rho, Y. D. 1996. Studies on the main factors affecting the mycelial growth of Phellinus lintenus. Kor. J. Mycol. 24:214-222.
16 Choi, H. S., Kim, M. K., Park, H. S., Kim, J. S., Shen, M. H. and Kim, S. J. 2005. Screening of fibrinolytic activities from cultured mushrooms. Korean J. Food Sci. Technol. 37(6):1039- 1041.
17 Blois, M. S. 1958. Antioxidant determinations by the use of a stale free radical. Nature 181:1199-1120.   DOI   ScienceOn
18 Choi, H. J., Kim, N. J. and Kim, D. H. 2000. Hypoglycemic effect of GE974 isolated from Gyrophora esculenta in normal and diabetic mice. Kor. J. pharmacogn. 31(3):268- 272.