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http://dx.doi.org/10.4489/KJM.2002.30.1.023

The Genetic Variations of Pleurotus spp. on Subculture  

Kang, Kyung-Hong (School of Natural Science, Jeonju University)
Song, Ju-Hee (Department of Biology, Graduate School, Jeonju University)
Kim, Hong-Nam (The Natural Science Research Insititute, Jeonju University)
Publication Information
The Korean Journal of Mycology / v.30, no.1, 2002 , pp. 23-30 More about this Journal
Abstract
The genetic variations and the rate of mycelial growth in the dikaryon and the monokaryon stages of Pleurotus spp. were surveyed during their subcultures. The highest growth rate was observed on both the 3rd and the 4th subcultures. The remarkably rapid growth rate was detected in P. ostreatus dikaryon. Genetic similarities in the dikaryon and the monokaryon of P. ostreatus were more than 57.5% and 85.7%, respectively, and those of P. eryngii were more than 85.2% and 84.8%, respectively. The genetic similarities of monokaryotic P. ostreatus were higher than those of dikaryotic. The topology of phylogenetic trees showed that the divergence and the clustering patterns of branch did not correlated with the number of subcultures. This suggests that genetic variations occur very randomly on mycelial cultures. These results suggest that the monokaryotic mycelia is genetically more stable than the dikaryotic in subcultures, and that it is very useful to stock monokaryotic mycelia for making spawns and breeding of Pleurotus spp.
Keywords
Genetic similarity; Genetic variations; Growth rate; Pleurotus spp.; RAPD; Subculture;
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