Application of Photosynthetic Pigment Analysis Using a HPLC and CHEMTAX Program to Studies of Phytoplankton Community Composition |
Lee, Yong-Woo
(Climate & Marine Environment Team, Korea Marine Environment Management Corporation)
Park, Mi-Ok (Department of Oceanography, Pukyong National University) Kim, Yoon-Suk (Ocean Science and Technology Institute, Pohang University Science and Technology) Kim, Seong-Su (Climate & Marine Environment Team, Korea Marine Environment Management Corporation) Kang, Chang-Keun (Ocean Science and Technology Institute, Pohang University Science and Technology) |
1 | Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjornland, D. Repeta and N. Welschmeyer, 1991. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Mar. Ecol. Prog. Ser., 77: 183-196. DOI |
2 | Zapata, M., F. Rodríguez and J.L. Garrido, 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase column and pyridinecontaining mobile phases. Mar. Ecol. Prog. Ser., 195: 29-45. DOI |
3 | Mackey, M.D., D.J. Mackey, H.W. Higgins and S.W. Wright, 1996. CHEMTAX - a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Mar. Ecol. Prog. Ser., 144: 265-283. DOI |
4 | Mantoura, R.F.C. and C.A. Llewellyn, 1983. The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography. Anal. Chim. Acta, 151: 297-314. DOI ScienceOn |
5 | Oh, H.T., D.J. Kim, W.C. Lee, R.H. Jung, S.J. Hong, Y.S. Kang, Y.W. Lee and C. Tilburg, 2008. Composition of phytoplankton in Gamak Bay by CHEMTAX analyses. J. Environ. Sci., 17: 1155-1167. DOI |
6 | Park, M.O. and J.S. Park, 1997. HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. J. Korean Soc. Oceanogr., 32: 46-55. |
7 | Sabine, C.L., R.A. Feely, N. Gruber, R.M. Key, K. Lee, J.L. Bullister, R. Wanninkhof, C.S. Wong, D.W.R. Wallace, B. Tilbrook, F.J. Millero, T.H. Peng, A. Kozyr, T. Ono and A.F. Rios, 2004. The oceanic sink for anthropogenic . Science, 305: 367-371. DOI ScienceOn |
8 | Schluter, L., F. Mohlenberg, H. Havskum and S. Larsen, 2000. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas: testing the influence of light and nutrients on pigment/chlorophyll a ratios. Mar. Ecol. Prog. Ser., 192: 49-63. DOI |
9 | Strickland, J.D. and T.R. Parsons, 1972. A practical handbook of seawater analysis. 2nd ed. Bull. Fish. Res. Bd. Can., pp. 167. |
10 | Welschmeyer, N.A. and N. Hoepffner, 1986. Rapid xanthophyll cycling: an in situ tracer for mixing in the upper ocean. EOS (Trans. Am. Geophys. Un.), 67: 969. |
11 | Wright, S.W. and R.L. van den Enden, 2000. Phytoplankton community structure and stocks in the East Antarctic marginal ice zone (BROKE survey, January-March 1996) determined by CHEMTAX analysis of HPLC pigment signatures. Deep-Sea Res. II, 47:2363-2400. DOI ScienceOn |
12 | Lee, Y.W. and G. Kim, 2007. Linking groundwater-borne nutrients and dinoflagellate red-tide outbreaks in the southern sea of Korea using a Ra tracer. Estuar. Coast. Shelf Sci., 71: 309-317. DOI ScienceOn |
13 | Lee, Y.W., G. Kim, W.A. Lim and D.W. Hwang, 2010. A relationship between submarine groundwater-borne nutrients traced by Ra isotopes and the intensity of dinoflagellate red-tides occurring in the southern sea of Korea. Limnol. Oceanogr., 55: 1-10. DOI ScienceOn |
14 | Lee, Y.W., J.M. Lee and G. Kim, 2009b. Identifying sharp hydrographical changes in phytoplankton community structure using HPLC pigment signatures in coastal waters along Jeju Island, Korea. Ocean Sci. J., 44: 1-10. DOI ScienceOn |
15 | Leterlier, R.M., R.R. Bidigare, D.V. Hebel, M. Ondrusek, C.D. Winn and D.M. Karl, 1993. Temporal variability of phytoplankton community structure based on pigment analysis. Limnol. Oceanogr., 38: 1420-1437. DOI ScienceOn |
16 | Lohrenz, S.E., C.L. Carroll, A.D. Weidemann and M. Tuel, 2003. Variations in phytoplankton pigments, size structure and community composition related to wind forcing and water mass properties on the North Carolina inner shelf. Cont. Shelf Res., 23:1447-1464. DOI ScienceOn |
17 | Mackey, D.J., H.W. Higgins, M.D. Mackey and D. Holdsworth, 1998. Algal class abundances in the western equatorial Pacific: Estimation from HPLC measurements of chloroplast pigments using CHEMTAX. Deep-Sea Res. I, 45: 1441-1468. DOI ScienceOn |
18 | Irigoien, X., B. Meyer, R. Harris and D. Harbour, 2004. Using HPLC pigment analysis to investigate phytoplankton taxonomy: the importance of knowing your species. Helgol. Mar. Res., 58: 77-82. DOI ScienceOn |
19 | Jeffrey, S.W., 1997. Application of pigment methods to oceanography. In: Jeffery, S.W., Mantoura, R.F.C., Wright, S.W. (eds) Phytoplankton pigments in oceanography: guidelines to modern methods, UNESCO Publishing, Paris. |
20 | Karl, D.M., 1999. A sea of change: Biogeochemical variability in the North Pacific subtropical gyre. Ecosystems, 2: 181-214. DOI ScienceOn |
21 | Latasa, M. and E. Berdalet, 1994. Effect of nitrogen or phosphorus starvation on pigment composition of cultured Heterocapsa sp. J. Plankton Res., 16: 83-94. DOI ScienceOn |
22 | Lee, Y.W., D.W. Hwang, G. Kim, W.C. Lee and H.T. Oh, 2009a. Nutrient inputs from submarine groundwater discharge (SGD) in Masan Bay, an embayment surrounded by heavily industrialized cities, Korea. Sci. Total Environ., 407: 3181-3188. DOI ScienceOn |
23 | Gieskes, W.W. and G.W. Kraay, 1983. Unknown chlorophyll a derivatives in the North Sea and the tropical Atlantic Ocean revealed by HPLC analysis. Limnol. Oceanogr., 28: 757-766. DOI ScienceOn |
24 | Goericke, R. and J.P. Montaya, 1998. Estimating the contribution of microalgal taxa to chlorophyll a in the field-variations of pigment ratios under nutrient- and light-limited growth. Mar. Ecol. Prog. Ser., 169: 97-112. DOI |
25 | Hassen, M.B., Z. Drira, A. Hamza, H. Ayadi, F. Akrout and H. Issaoui, 2008. Summer phytoplankton pigments and community composition related to water mass properties in the Gulf of Gabes. Estuar. Coast. Shelf Sci., 77: 645-656. DOI ScienceOn |
26 | Holm-Hansen, O., C.J. Lorenzen, R.W. Holmes and J.D.H. Strickland, 1965. Fluorometric determination of chlorophyll. J. Cons. int. Explor. Mer., 30: 3-15. DOI |
27 | Bidigare, R.P., T.J. Frank, C. Zastrow and J.M. Brooks, 1986. The distribution of algal chlorophylls and their degradation products in the Southern Ocean. Deep-Sea Res., 33: 923-937. |
28 | Burkill, P.H., R.F.C. Mantoura, C.A. Llewellyn and N.J.P. Owens, 1987. Microzooplankton grazing and selectivity of phytoplankton in coastal waters. Mar. Biol., 93: 581-590. DOI ScienceOn |
29 | Everitt, D.A., S.W. Wright, J.K. Volkman, D.P. Thomas and E.J. Lindstrom, 1990. Phytoplankton community compositions in the Southern Western equatorial Pacific determined from chlorophyll and carotenoid pigment distributions. Deep-Sea Res., 37: 975-997. DOI ScienceOn |
30 | Furuya, K., M. Hayashi, Y. Yabushita and A. Ishikawa, 2003. Phytoplankton dynamics in the East China Sea in spring and summer as revealed by HPLC-derived pigment signatures. Deep-Sea Res. II, 50: 367-387. DOI ScienceOn |
31 | Anderson, R.A., R.R. Bidigare, M.D. Keller and M. Latasa, 1996. A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific Oceans. Deep-Sea Res. II, 43: 517-537. DOI ScienceOn |
32 | Azam, F., T. Fenchel, J.G. Field, J.S. Gray, L.A. Meyer-Reil and F. Thingstad, 1983. The ecological role of water-column microbes in the sea. Mar. Ecol. Prog. Ser., 10: 257-263. DOI |