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http://dx.doi.org/10.7850/jkso.2011.16.3.117

Application of Photosynthetic Pigment Analysis Using a HPLC and CHEMTAX Program to Studies of Phytoplankton Community Composition  

Lee, Yong-Woo (Climate & Marine Environment Team, Korea Marine Environment Management Corporation)
Park, Mi-Ok (Department of Oceanography, Pukyong National University)
Kim, Yoon-Suk (Ocean Science and Technology Institute, Pohang University Science and Technology)
Kim, Seong-Su (Climate & Marine Environment Team, Korea Marine Environment Management Corporation)
Kang, Chang-Keun (Ocean Science and Technology Institute, Pohang University Science and Technology)
Publication Information
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY / v.16, no.3, 2011 , pp. 117-124 More about this Journal
Abstract
Many studies of the phytoplankton community structure have been conducted using the CHEMTAX program on the basis of the photosynthetic pigment concentrations measured by a HPLC (High-Performance Liquid Chromatography) technique. The CHEMTAX program determines the contribution of each phytoplankton class to total phytoplankton biomass (chlorophyll a) based on the ratios of marker pigment to chlorophyll a of phytoplankton group. In this study, the marker pigment/chlorophyll a ratios were investigated in phytoplankton species isolated from marine waters around the Korean peninsula. These results were used as the input pigment ratios of the CHEMTAX program to investigate phytoplankton community structure in Korean coastal waters (Yeoja and Gamak Bay). There were significant differences in the ratios of marker pigment to chlorophyll a among the different species within the same algal class. There was a significant difference between the values of our ratios and the previously used ratios in other regions of the world. When phytoplankton community composition was calculated using our initial ratios in Yeoja and Gamak Bay, our results were significantly different from the results calculated on the basis of initial ratios of marker pigment in phytoplankton suggested in other marine waters. The estimates of the contributions of the major algal groups (bacillariophyceae and dinophytes) to total chlorophyll a varied within 5% depending on the initial ratios chosen. The variations of estimates for the pico- and nanoplankton (cyanophytes and prasinophytes), which have relatively low contributions to total chlorophyll a, were higher than those for major algal group. Although the HPLC-pigment measurements combined with CHEMTAX analysis are useful for identifying and qualifying phytoplankton community structure, further researches for the pigment ratios of the dominant phytoplankton species presenting in a given area are also needed.
Keywords
HPLC; CHEMTAX; Phytoplankton community structure; Photosynthetic pigment; Yeoja Bay; Gamak Bay;
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