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M. tuberculosis Somatic Antigen Specific CD8+T cell Responses in BCG-Vaccinated Subjects  

Cho, Jang-Eun (Department of Microbiology, Brain Korea 21 project for Medical Sciences, Yonsei University Medical Center)
Cho, Sang-Nae (Department of Microbiology, Brain Korea 21 project for Medical Sciences, Yonsei University Medical Center)
Lee, Kyung Wha (Hallym Institution for Genome Application Hallym University)
Park, Seung Kyu (National Masan Tuberculosis Hospital)
Cho, Sungae (Department of Microbiology, Brain Korea 21 project for Medical Sciences, Yonsei University Medical Center)
Publication Information
Tuberculosis and Respiratory Diseases / v.59, no.3, 2005 , pp. 272-278 More about this Journal
Abstract
Background : The immune responses mediated by CD8+T cells are known to be significant in controlling M. tuberculosis infections. In order to determine the role of cytotoxic CD8+T cells in the protective immune mechanism in latently infected subjects, this study examined whether or not the cytotoxic immune responses of CD8+T cells specific to the M. tuberculosis somatic antigens are induced in BCG vaccinated healthy subjects. Methods : Cytotoxicity and $IFN-{\gamma}$ elispot assays were used to investigate the activities of CD8+T cells specific for the $thyA_{30-38}$ peptide epitope in circulating peripheral blood mononuclear cells (PBMC) from BCG-vaccinated HLA-A*0201 and A*0206 subjects. Results : The results indicate the cytotoxic and $IFN-{\gamma}$ immune responses of CD8+T cells specific for $thyA_{30-38}$ were induced in BCG vaccinated healthy subjects. Conclusion : The cytotoxic and $IFN-{\gamma}$ responses by CD8+T cells specific for the M. tuberculosis somatic antigens are induced in BCG-vaccinated subjects, and appear to be involved in the protective immune mechanism in latently infected people against a M. tuberculosis infection.
Keywords
CD8+T cells; cytotoxicity; M. tuberculosis somatic antigens;
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1 Smith SM, Brookes R, Klein MR, Malin AS, Lukey PT, King AS, et al. Human CD8+ CTL specific for the mycobacterial major secreted antigen 85A. J Immunol 2000;165:7088-95
2 Cho JE, Lee KW, Park SK, Cheon SH, Cho SN, Cho S. The study of MHC calss I restricted CD8+ T cell mediated immune responses against Mycobacterium tuberculosis infection: evidence of M. tuberculosis specific CD8+ T cells in TB patients and PPD+ healthy individuals. Immune Netw 2003;3:235-41
3 Sette A, Vitiello A, Reherman B, Fowler P, Nayersina R, Kast WM, et al. The relationship between class I binding affinity and immunogenicity of potential cytotoxic T cell epitopes. J Immunol 1994;153:5586-92
4 Hurely C. Sequence-based typing for HLA-A. In: Tilanus MG, Hansen JA, Hurley CK, editors. Calss I sequencing amplification of exons 2 and 3 based upon Cereb. IHWG Technical Manual. Vol. TM7A. Seatle: International Histocompatibility Working Group; 2000
5 Murray PJ. Defining the requirements for immunological control of mycobacterial infections. Trends Microbiol 1999;7:366-72   DOI   PUBMED   ScienceOn
6 World Health Organization. WHO report on the tuberculosis epidermic. Publ. No. WHO/TB/95. Geneva: World Health Organization; 1995. p. 183
7 Flynn JL, Goldstein MM, Chan J, Triebold KJ, Pfeffer K, Lowenstein CJ, et al. Tumor necrosis factor-alpha is required in the protective immune response against Mycobacterium tuberculosis in mice. Immunity 1995; 2:561-72   DOI   ScienceOn
8 Chan J, Tanaka K, Carroll D, Flynn J, Bloom BR. Effects of nitric oxide synthase inhibitors on murine infection with Mycobacterium tuberculosis. Infect Immun 1995;63:736-40
9 Schnappinger D, Ehrt S, Voskuil MI, Liu Y, Mangan JA, Monahan IM, et al. Transcriptional adaptation of Mycobacterium tuberculosis within macrophages: insights into the phagosomal environment. J Exp Med 2003;198:693-704   DOI   ScienceOn
10 Lewinsohn DM, Zhu L, Madison VJ, Dillon DC, Fling SP, Reed SG, et al. Classically restricted human CD8+ T lymphocytes derived from Mycobacterium tuber culosis-infected cells: definition of antigenic specificity. J Immunol 2001;166:439-46   PUBMED
11 Canaday DH, Ziebold C, Noss EH, Chervenak KA, Harding CV, Boom WH. Activation of human CD8+ alpha beta TCR+ cells by Mycobacterium tuberculosis via an alternate class I MHC antigen-processing pathway. J Immunol 1999;162:372-9
12 Flynn JL, Chan J. Immunology of tuberculosis. Annu Rev Immunol 2001;19:93-129   DOI   ScienceOn
13 Tully G, Kortsik C, Hohn H, Zehbe I, Hitzler WE, Neukirch C, et al. Highly focused T cell responses in latent human pulmonary Mycobacterium tuberculosis infection. J Immunol 2005;174:2174-84
14 Chan J, Flynn J. The immunological aspects of latency in tuberculosis. Clin Immunol 2004;110:2-12   DOI   ScienceOn
15 Flynn JL. Why is IFN-gamma insufficient to control tuberculosis? Trends Microbiol 1999;7:477-8
16 Chen A, Wang L, Zhang J, Zou L, Jia Z, Zhou W, et al. H-2 Kd-restricted hepatitis B virus-derived epitope whose specific CD8+ T lymphocytes can produce gamma interferon without cytotoxicity. J Virol 2005;79:5568-76   DOI   ScienceOn
17 Pathan AA, Wilkinson KA, Wilkinson RJ, Latif M, McShane H, Pasvol G, et al. High frequencies of circulating IFN-gamma-secreting CD8 cytotoxic T cells specific for a novel MHC class I-restricted Mycobacterium tuberculosis epitope in M. tuberculosis-infected subjects without disease. Eur J Immunol 2000;30: 2713-21   DOI   ScienceOn
18 Lewinsohn DM, Alderson MR, Briden AL, Riddell SR, Reed SG, Grabstein KH, et al. Characterization of human CD8+ T cells reactive with Mycobacterium tuberculosis-infected antigen-presenting cells. J Exp Med 1998;187:1633-40   DOI   ScienceOn
19 van Pinxteren LA, Cassidy JP, Smedegaard BH, Agger EM, Andersen P. Control of latent Mycobacterium tuberculosis infection is dependent on CD8 T cells. Eur J Immunol 2000;30:3689-98   DOI   ScienceOn
20 Flynn JL, Chan J, Triebold KJ, Dalton DK, Stewart TA, Bloom BR. An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection. J Exp Med 1993;178:2249-54   DOI   PUBMED
21 Cho S, Mehra V, Thoma-Uszynski S, Stenger S, Serbina N, Mazzaccaro RJ, et al. Antimicrobial activity of MHC class I-restricted CD8+ T cells in human tuberculosis. Proc Natl Acad Sci U S A 2000;97:12210-5
22 Flynn JL. Immunology of tuberculosis and implications in vaccine development. Tuberculosis 2004;84:93-101   DOI   ScienceOn
23 del Guercio MF, Sidney J, Hermanson G, Perez C, Grey HM, Kubo RT, et al. Binding of a peptide antigen to multiple HLA alleles allows definition of an A2-like supertype. J Immunol 1995;154:685-93