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MMP-2 and MMP-9 are Differentially Involved in Molar Growth  

Kim, Min-Seok (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Kang, Jee-Hae (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Kim, Dong-Hoo (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Yoo, Hong-Il (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Jung, Na-Ri (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Yang, So-Young (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Lee, Eun-Ju (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Kim, Sun-Hun (Dental Science Research Institute, 2nd Stage Brain Korea, School of Dentistry, Chonnam National University)
Publication Information
International Journal of Oral Biology / v.36, no.4, 2011 , pp. 195-201 More about this Journal
Abstract
Matrix metalloproteinases (MMPs) have been implicated in tissue development and re-modeling. Dynamic morphological changes of tooth germs reflect involvement of these enzymes during odontogenesis. The present study was performed to investigate expression and localization of MMP-2 and MMP-9, which have been known to have type IV collagenase activities, in rat tooth germs at different developmental stages. MMP-2 expression was increased gradually in the tooth germs from cap to crown staged germs at both transcription and translation levels. The localization of this molecule was detected in secretory ameloblasts and preameloblasts. The strong immunoreactivities were occasionally seen along the basement membrane between ameloblasts (or preameloblasts) and odontoblasts (preodontoblasts). However, weak reactivity was detected in odontoblasts and reduced enamel epithelium. The level of MMP-9 expression in the tooth germs was higher in cap stage than in crown staged germs at both transcription and translation levels. They were strongly expressed in both ameloblasts and odontoblasts. Even though reduced enamel epithelium after enamel formation and inner enamel epithelium at the cap stage exhibited weak reactivity, strong reactivity was detected in dental follicles and perifollicular tissues surrounding cap staged germs. These results suggested that MMP-2 may involve degradation of the basement membrane during hard tissue formation, whereas MMP-9 might be involved in remodeling of follicular tissues.
Keywords
MMP-2; MMP-9; Molar;
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