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THE EFFECT OF PULSED ELECTROMAGNETIC FIELDS ON ${\beta}$-TCP GRAFT IN RABBIT CRANIAL BONE DEFECT  

Kim, Sang-Woo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
Hwang, Kyung-Gyun (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
Lim, Byung-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
Park, Chang-Joo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
Chung, Il-Hyuk (Department of Dentistry/Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital)
Paik, Seung-Sam (Department of Pathology, College of Medicine, Hanyang University)
Shim, Kwang-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.32, no.4, 2006 , pp. 360-373 More about this Journal
Abstract
The purpose of this research was to investigate whether pulsed electromagnetic field (PEMF) stimulation applied to the rabbit cranial defects grafted with ${\beta}$-tricalcium phosphate (${\beta}$-TCP) could affect the new bone formation. With 16 New Zealand white rabbits under the same condition, bilateral calvarial bone defects were formed around the sagittal suture line. The defect on the left side was grafted with ${\beta}$-TCP, while on the right side was grafted by harvested autogenous bone. PEMF was applied to 8 rabbits for 8 hours per day. The bony specimen were divided into 3 groups, the group 1 was autogenous bone grafted specimen, the group 2 was ${\beta}$-TCP grafted with PEMF, and the group 3 was ${\beta}$-TCP grafted without PEMF. We investigated the bone regeneration & growth factor expression at 2, 4, 6, and 8 weeks. As a result, BMP 2 was expressed in the group 1 from 2 weeks, the group 2 from 4 weeks, and the group 3 from 6 weeks. BMP 4 was expressed in the group 1 from 2 weeks, in the group 2 and the group 3 from 4 weeks. 4. There was no significant difference in expression pattern of BMP 7, PDGF, VEGF, and TGF-${\beta}$1 during grafted bone regeneration in group 1, 2, and 3. According to our results, PEMF stimulation could be effective on the new bome formation in animal study, and have a feasibility of clinical use.
Keywords
Pulsed electromagnetic field (PEMF); Autogenous bone; ${\beta}$-tricalcium phosphate (${\beta}$-TCP); Osteogenesis;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Merten HA, Wiltfang J, Grohmann U et al: Intraindividual Comparative animal Study of ${\alpha}$-and ${\beta}$-Tricalcium Phosphate Degradation in Conjunction with Simultaneous Insertion of Dental Implants. J Craniofac Surg 2001;12:59-68   DOI
2 Yasuda I: Mechanical and electrical callus. Ann NY Sci 1974;238:457   DOI   ScienceOn
3 Lu J, Descamps M, Dejou J et al: The biodegradation mechanism of calcium phosphate biomaterials in bone. J Biomed Bater Res 2002;63:408-412   DOI   ScienceOn
4 Spadoro JA: Bioelectrical properties of bone and response of bone to electrical stimuli. In Bone. Vol 3, Boston, CRC Press, 1991;109-141
5 Happenstall BR: Constant direct current treatment for established non-union of the tibia. Clin Orthop 1983;178:179
6 Buzza EP, Shibli JA, Barbeiro RH, Barbosa JR: Effects of electromagnetic field on bone healing around commercially pure titanium surface: histologic mechanical study in rabbits. Implant Dent 2003;12:182-7   DOI   ScienceOn
7 Grissett JD: Biological effects of electric and magnetic fields associated with ELF communications systems, Proceeding of IEEE 1980;68:98-104
8 Street J, Bao M, deGuzman L et al: Vascular endotherial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. PNAS 2002;99:9656-9661
9 Bassett CAL, Pilla AA, and Pawluk RJ: A nonoperative salvage of surgically-resistant pseudoarthrosis and non-union by pulsating electromagnetic fields. Clin Orthop 1977;124:128-143
10 Steiner M, Ramp WK: Electrical stimulation of bone and its implications for endosseous dental implantation. J Oral Implant 1990;16:20- 27
11 Stenport VF, Johansson C, Heo SJ et al: Titanium implants and BMP- 7 in bone: an experimental model in the rabbit. J Mater Sci Mater Med 2003;14:247-254   DOI   ScienceOn
12 Roden GA, Bourrett LA, Norton LA: DNA sytheses in cartilage cell stimulated by oscillating eletrical field. Science 1978;199:690   DOI
13 Norton LA: Implication of bioelectric growth control in orthodontics and dentistry. Angle Orthod 1975;45:34-42
14 Bahn S, Plaster: A Bone substitute. Oral Surg Oral Med Oral Pathol 1966;21:672-681   DOI   ScienceOn
15 Lee SH, Song SI, Hwang KG et al: The experimental study of the regeneration on ${\beta}$-TCP in rabbit cranial bone. J Kor Oral Maxillofac Surg 2004;30:282-291   과학기술학회마을
16 Branham GB, Triplett RG, Yeandle S, Vieras F: The effect of electrical current on the healing of mandibular freezed dried bone allograft in dog. J Oral Maxillofac Surg 1985;43:403-407   DOI
17 Hass DW: Pulsating electromagnetic current induction of mandibular condyles in the cat. J Dent Res 1984;63-335
18 Urist MR, Raskin K, Goltz D et al: Endogenous bone morphogenic protien : immunohistochemical localization in repair of a punch hole in the rabbit's ear : Plast Reconstr Surg 1997;99:1382-1389   DOI
19 Fini M, Cadossi R, Cane V, Cavani F, Giavaresi G, Krajewski A, Martini L, Aldini NN, Ravaglioli A, Rimondini L, Torricelli P, Giardino R: The effect of pulsed electromagnetic fields on the osteointegration of hydroxyapatite implants in cancellous bone: a morphologic and microstructural in vivo study. J Orthop Res 2002;20:756-63   DOI   ScienceOn
20 Szabo G, Suba Z, Hrabak K et al: Autogenous Bone Versus ${\beta}$- Tricalcium Phosphate Graft Alone for Bilateral Sinus Elevations(2- and 3-Dimensional Computed Tomographic, Histologic, and Histomorphometric Evaluations): Preliminary Results. Int J Oral Maxillofac Implant 2001;16:681-692
21 Watanabe K, Niimi A, Ueda M: Autogenous bone grafts in the rabbit maxillary sinus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;88:26-32   DOI   ScienceOn
22 Wiltfang J, Merten HA, Schlegel KA et al: Degradation characteristics of alpha and beta tri-calcium phosphate in minipigs. J Biomed Mater res 2002;63:115-121   DOI   ScienceOn
23 Matsumoto H, Ochi M, Abiko Y, Kaku T, Sakaguchi K: Pulsed electromagnetic fields promote bone formation around dental implants inserted into the femur of rabbits. Clin Oral Implants Res 2000;11:354-60   DOI   ScienceOn
24 Laurencin CT: Bone Graft Substitutes. ASTM International 2003;180-187
25 Braden M, Bairstow AG, Beider A, ritter BG: Electrical and piezoelectrical properties of dental hard tissue. Nature 1966;212:1565- 1566   DOI
26 Cochran GVB, Pawluk RJ, Bassett CAL: Stress generated electrical potentials in the mandible and teeth. Arch Oral Biol 1967;12:917- 920   DOI   ScienceOn
27 Peltier LF: A brief historical note on the use of electricity in the treatment of fracture. Clin Orthop 1981;161:4-7
28 Yasuda I: Piezoelectricity of living bone. J Kyoto Pref Univ Med 1953;53:325
29 Vingerling PA, Vanderkuij P, Degroot K, Sillevis PAE: Non-invasive treatment of alveolar wounds. In Electrical properties of bone and cartilage. Graune and Stratton. New York 1979;341-346
30 Ducheyne P, Qiu Q: Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. Biomaterials 1999;20:2287-303   DOI   ScienceOn
31 Kloen P, Di Paola M, Borens O et al: BMP signaling components are expressed in human fracture callus. Bone 2003;33:362-371   DOI   ScienceOn
32 Bolander MR: Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 1992;200:165-170
33 Norton LA, Hanley KJ, Turkewicz J: Bioelectric pertubations of bone. Angle Orthod 1984;54:73-87
34 Bassett CAL: The development and application of pulsed electromagnetic fields(PEMFs) for ununited fractures and arthrodeses. Orthop Clin North Am 1984;15:61-88
35 Gerling JA, Sinclair PM, Roa RL: The effect of pulsating electromagnetic fields on condylar growth in guinea pigs. Am J Orthop 1985;87:211-223   DOI   ScienceOn
36 Saito M, Shimizu H, Beppu M et al: The role of ${\beta}$-Tricalcium Phosphate in vasculized periosteum. J Orthop Sci 2000;5:275-282   DOI
37 Norton LA, Rodan GA, Bourret LA: Epiphyseal cartilage c-AMP changes produced by electrical and mechanical perturbations. Clin Orthop 1977;124:59-68