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Polysaccharides from Edible Mushroom Hinmogi (Tremella fuciformis) Inhibit Differentiation of 3T3-L1 Adipocytes by Reducing mRNA Expression of $PPAR{\gamma}$, C/$EBP{\alpha}$, and Leptin  

Jeong, Hye-Jin (Department of Biotechnology, Yonsei University)
Yoon, Seon-Joo (Graduate Program in Biomaterials Science and Engineering, Yonsei University)
Pyun, Yu-Ryang (Department of Biotechnology, Yonsei University)
Publication Information
Food Science and Biotechnology / v.17, no.2, 2008 , pp. 267-273 More about this Journal
Abstract
Water-soluble fraction (WSF) from edible mushroom hinmogi (Tremella fuciformis) were obtained by water extraction, and polysaccharides in the WSF were separated by ethanol precipitation. The inhibitory effects of the polysaccharides on 3T3-L1 adipocyte differentiation were evaluated by the reduction of peroxisome proliferators-activated receptor ${\gamma}$ ($PPAR{\gamma}$) translation, triglyceride accumulation, Oil Red-O staining, and expression levels of $PPAR{\gamma}$, CCAAT/enhancer binding protein a (C/$EBP{\alpha}$), and leptin. The $PPAR{\gamma}$ translation in 3T3-L1 cells was inhibited by the treatment with polysaccharide precipitated by 80% ethanol (P80) which showed highest inhibitory activity among polysaccharides tested. In addition, treatment of P80 to 3T3-L1 cells significantly inhibited the triglyceride accumulation, Oil Red-O staining, and mRNA expression of $PPAR{\gamma}$, C/$EBP{\alpha}$, and leptin in a dose-dependent manner. Based upon these results, P80 from edible mushroom hinmogi shows the inhibitory activity on the differentiation of 3T3-L1 adipocytes. Therefore, it might be employed as a potential anti-obesity material.
Keywords
edible mushroom; Tremella fuciformis; polysaccharide; differentiation; anti-obesity;
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Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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1 Guo FC, Kwakkel RP, Williams BA, Parmentier HK, Li WK, Yang ZQ, Verstegen MW. Effects of mushroom and herb polysaccharides on cellular and humoral immune responses of Eimeria tenellainfected chickens. Poultry Sci. 83: 1124-1132 (2004)   DOI
2 Kiho T, Tsujimura Y, Sakushima M, Usui S, Ukai S. Polysaccharides in fungi. XXXIII. Hypoglycemic activity of an acidic polysaccharide (AC) from Tremella fuciformis. Yakuga. Zasshi 114: 308-315 (1994)   DOI
3 Yoon S-J, Pereira MS, Pavao MSG, Hwang J-K, Pyun Y-R, Mourao PAS. The medicinal plant Porana volubilis contains polysaccharides with anticoagulant activity mediated by heparin cofactor II. Thromb. Res. 106: 51-58 (2002)   DOI   ScienceOn
4 Vankoningsloo S, Piens M, Lecocq C, Gilson A, De Pauw A, Renard P, Demazy C, Houbion A, Raes M, Arnould T. Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: Role of fatty acid beta-oxidation and glucose. J. Lipid Res. 46: 1133-1149 (2005)   DOI   ScienceOn
5 Wu Z, Bucher NL, Farmer SR. Induction of peroxisome proliferatoractivated receptor ${\gamma}$ during the conversion of 3T3 fibroblasts into adipocytes is mediated by $C/EBP{\beta}$ , $C/EBP{\zeta}$, and glucocorticoids. Mol. Cell Biol. 16: 4128-4136 (1996)   DOI
6 Laville M, Andreelli F. Mechanisms for weight gain during blood glucose normalization. Diabetes Metab. 26: 42-45 (2000)
7 Mandrup S, Lane MD. Regulating adipogenesis. J. Biol. Chem. 272: 5367-5370 (1997)   DOI
8 Kawada T, Takahashi N, Fushiki T. Biochemical and physiological characteristics of fat cell. J. Nutr. Sci. Vitaminol. 47: 1-12 (2001)   DOI   ScienceOn
9 Guo FC, Kwakkel RP, Williams CB, Suo X, Li WK, Verstegen MW. Coccidiosis immunization: Effects of mushroom and herb polysaccharides on immune responses of chickens infected with Eimeria tenella. Avian Dis. 49: 70-73 (2005)   DOI   ScienceOn
10 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. A colorimetric method for determination of sugars. Nature 168: 167 (1951)
11 Bellini MF, Angeli JP, Matuo R, Terezan AP, Ribeiro LR, Mantovani MS. Antigenotoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in )$CHO-{\kappa}1$ CHO-k1 and HTC cells. Toxicol. In Vitro 20: 355-360 (2006)   DOI   ScienceOn
12 Kim K-A, Chang H-Y, Choi S-W, Yoon J-W, Lee C. Cytotoxic effects of extracts from Tremella fuciformis strain FB001 on the human colon adenocarcinoma cell line DLD-1. Food Sci. Biotechnol. 15: 889-895 (2006)   과학기술학회마을
13 Wise LS, Green H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J. Biol. Chem. 254: 273-275 (1979)
14 Chung M-J, Lee Y-S, Kim B-C, Lee S-B, Moon T-W, Lee S-J, Park K-H. The hypoglycemic effects of Acarviosine-glucose modulate hepatic and intestinal glucose transporter in vivo. Food Sci. Biotechnol. 16: 851-855 (2006)   과학기술학회마을
15 Lin FT, Lane MD. Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. Genes Dev. 6: 533-544 (1992)   DOI   ScienceOn
16 Kallen CB, Lazar MA. Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3-L1 adipocytes. P. Natl. Acad. Sci. USA 93: 5793-5796 (1996)   DOI   ScienceOn
17 Rubin CS, Hirsh A, Fung C, Rosen OM. Development of hormone receptors and hormonal responsiveness in vitro: Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells. J. Biol. Chem. 253: 7570-7578 (1978)
18 Hong KM, Belperio JA, Keane MP, Murdick MD, Strieter RM. Differentiation of human circulating fibrocytes as mediated by transforming growth $factor-{\beta}$ and peroxisome proliferator-activated receptor ${\gamma}$ . J. Biol. Chem. 282: 22910-22920 (2007)   DOI   ScienceOn
19 Vankoningsloo S, Pauw AD, Houbion A, Tejerina S, Demazy C, de Longueville F, Bertholet V, Renard P, Remacle J, Holvoet P, Raes M, Arnould T. CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes. J. Cell Sci. 119: 1266-1282 (2006)   DOI   ScienceOn
20 Yoon S-J, Yu M-A, Pyun Y-R, Hwang J-K, Chu DC, Juneja LR, Mourao PAS. The nontoxic mushroom Aurucularia auricula contains a polysaccharide with anticoagulant activity mediated by antithrombin. Thromb. Res. 112: 151-158 (2003)   DOI   ScienceOn
21 Takahashi N, Kawada T, Goto T, Yamamoto T, Taimatsu A, Matsui N, Kimura K, Saito M, Hosokawa M, Miyashita K, Fushiki T. Dual action of isoprenols from herbal medicines on both $PPAR{\gamma}$ and $PPAR{\alpha}$ in 3T3-L1 adipocytes and HepG2 hepatocytes. FEBS Lett. 514: 315-322 (2002)   DOI   ScienceOn
22 Kim H-S, Liang L, Dean RG, Hausman DB, Hartzell DL, Baile CA. Inhibition of preadipocyte differentiation by myostatin treatment in 3T3-L1 cultures. Biochem. Bioph. Res. Co. 281: 902-906 (2001)   DOI   ScienceOn
23 Mun E-G, Soh J-R, Cha Y-S. L-Carnitine reduces obesity caused by high-fat diet in C57BL/6J mice. Food Sci. Biotechnol. 16: 228-233 (2006)   과학기술학회마을
24 Dische Z. A new specific color reaction of hexuronic acid. J. Biol. Chem. 167: 189-198 (1947)
25 Harmon AW, Harp JB. Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. Am. J. Physiol.-Cell. Ph. 280: C807-C813 (2001)   DOI
26 Ukai S, Kiriki H, Nagai K, Kiho T. Synthesis and antitumor activities of conjugates of mitomycin C-polysaccharide from Tremella fuciformis. Yakuga. Zasshi 112: 663-668 (1992)   DOI
27 Takahashi N, Kawada T, Yamamoto T, Goto T, Taimatsu A, Aoki N, Kawasaki H, Taira K, Yokoyama KK, Kamei Y, Fushiki T. Overexpression and ribozyme-mediated targeting of transcriptional coactivators CREB-binding protein and p300 revealed their indispensable roles in adipocyte differentiation through the regulation of peroxisome proliferators-activated receptor gamma. J. Biol. Chem. 277: 16906-16912 (2002)   DOI   ScienceOn
28 Borchers AT, Keen CL, Gershwin ME. Mushrooms, tumors, and immunity: An update. Exp. Biol. Med. 229: 393-406 (2004)   DOI