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Effects of Tributyltin Chloride on L-DOPA-Induced Cytotoxicity in PC12 Cells  

Lee, Jae-Joon (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Kim, Yu-Mi (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Park, Seung-Kook (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Archives of Pharmacal Research / v.29, no.8, 2006 , pp. 645-650 More about this Journal
Abstract
Tributyltin chloride (TBTC) at concentrations of $0.5-1.0\;{\mu}M$ inhibits dopamine biosynthesis in PC12 cells. In this study, the effects of TBTC on L-3,4-dihydroxyphenylalanine (L-DOPA)-induced cytotoxicity in PC12 cells were investigated. TBTC at concentrations up to $1.0\;{\mu}M$ neither affected cell viability, nor induced apoptosis after 24 or 48 h in PC12 cells. However, TBTC at concentrations higher than $2.0\;{\mu}M$ caused cytotoxicity through an apoptotic process. In addition, exposure of PC12 cells to non-cytotoxic (0.5 and $1.0\;{\mu}M$) or cytotoxic $(2.0\;{\mu}M)$ concentrations of TBTC in combination with L-DOPA (20, 50 and $100\;{\mu}M$) resulted in a significant increase in cell loss and the percentage of apoptotic cells after 24 or 48 h compared with TBTC or L-DOPA alone. The enhancing effects of TBTC on L-DOPA-induced cytotoxicity were concentration- and treatment time-dependent. These data demonstrate that TBTC enhances L-DOPA-induced cytotoxicity in PC 12 cells.
Keywords
Tributyltin chloride; L-3,4-Dihydroxyphenylalanine (L-DOPA); Cytotoxicity; Apoptosis; PC12 cells;
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1 Mailman, R. B. and Lewis, M. H., Neurotoxicants and central catecholamine systems. Neurotoxicol., 8, 123-139 (1987)
2 Fent, K., Ecotoxicology of organotin compounds. Crit. Rev. Toxicol., 26, 1-117 (1996)
3 Greene, L. A. and Tischler, A. S., Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. U.S.A., 73, 2424-2428 (1976)
4 Gennari, A., Viviani, B., Galli, C. L., Marinovich, M., Pieters, R., and Corsini, E., Organotins induce apoptosis by disturbance of $[Ca^{2+}]_i$ and mitochondrial activity, causing oxidative stress and activation of caspases in rat thymocytes. Toxicol. Appl. Pharmacol., 169, 185-190 (2000)   DOI   ScienceOn
5 Kim, Y. M., Lee, J. J., Yin, S. Y., Kim, Y. S., Lee, J. K., Yoon, Y. P., Kang, M. H., and Lee, M. K., Inhibitory effects of tributyltin on dopamine biosynthesis in rat PC12 cell. Neurosci. Lett., 332, 13-16 (2002)   DOI   ScienceOn
6 Sandler, M., Carter, S. B., Hunter, K. R., and Stern, G. M., Tetrahydroisoquinoline alkaloids: in vivo metabolites of LDOPA in man. Nature, 241, 439-443 (1973)   DOI   ScienceOn
7 Tsunashima, K., Sadamatsu, M., Takahashi, Y., Kato, N., and Sperk, G., Trimethyltin intoxication induces marked changes in neuropeptide expression in the rat hippocampus. Synapse, 29, 333-342 (1998)   DOI   ScienceOn
8 Migheli, R., Godani, C., Bciola, L., Delodu, M. R., Serra, P. A., Zangani, D., Natale, G. D., Miele, E., and Desole, M. S., Enhancing effect of manganese on L-DOPA-induced apoptosis in PC12 cells: role of oxidative stress. J. Neurochem., 73, 1155-1163 (1999)   DOI
9 Agid, Y., Parkinson's disease: pathophysiology. Lancet, 337, 1321-1324 (1991)   DOI   ScienceOn
10 Cheng, N. N., Maeda, T., Kume, T., Kaneko, S., Kochiyama, H., Akaike, A., Goshima, Y., and Misu, Y., Differential neurotoxicity induced by L-DOPA and dopamine in cultured striatal neurons. Brain Res., 743, 278-283 (1996)   DOI   ScienceOn
11 Jenkins, S. M. and Barone Jr, S., The neurotoxicant trimethyltin induces apoptosis via caspase activation, p38 protein kinase, and oxidative stress in PC12 cells. Toxicol. Lett., 147, 63-72 (2004)   DOI   ScienceOn
12 Yin, S. Y., Kim, Y. M., Lee, J. J., Jin, C. M., Yang, Y. J., Ma, M. H., Kang, M. H., Kai, M., and Lee, M. K., Enantio-selective inhibition of (1R,9S)- and (1S,9R)-${\beta}$-hydrastines on dopamine biosynthesis in PC12 cells. Neuropharmacol., 47, 1045-1052 (2004)   DOI   ScienceOn
13 Boyce, S., Rupniak, N. M., Steventon, M. J., and Iversen, S. D., Nigrostriatal damage is required for induction of dyskinesias by L-DOPA in squirrel monkeys. Clin. Neuropharmacol., 13, 448-458 (1990)   DOI   ScienceOn
14 Reader, S., Moutardier, V., and Denizeau, F., Tributyltin triggers apoptosis in trout hepatocytes: the role of $Ca^{2+}$, protein kinase C and proteases, Biochim. Biophys. Acta., 1448, 473- 485 (1999)   DOI   ScienceOn
15 Wang, W. W., Post, J. I., Dow, K. E., Shin, S. H., Riopelle, R. J., and Ross, G. M., Zinc and copper inhibit nerve-growth factormediated protection from oxidative stress-induced apoptosis. Neurosci. Lett., 259, 115-118 (1999)   DOI   ScienceOn
16 Mizuhashi, S., Ikegaya, Y., and Matsuki, N., Cytotoxicity of tributyltin in rat hippocampal slice cultures. Neurosci. Res., 38, 35-42 (2000)   DOI   ScienceOn
17 Cheng, Y., Wixom, P., Jame-Kracke, M.R., and Sun, A.Y., Effects of extracellular ATP on $Fe^{2+}$-induced cytotoxicity in PC12 cells. J. Neurochem., 63 (1994) 895-902   DOI   ScienceOn
18 Mosman, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Methods., 65, 55-63 (1983)   DOI   ScienceOn
19 Basma, A. N., Morris, E. J., Nicklas, W. J., and Geller, M. H., LDOPA cytotoxicity to PC12 cells in cultures is via its autoxidation. J. Neurochem., 64, 825-832 (1995)   DOI
20 Stridh, H., Kimland, M., Jones, D. P., Orrenius, S., and Hampton, M. B., Cytochrome c release and caspase activation in hydroxide- and tributyltin-induced apoptosis. FEBS Lett., 429, 351-355 (1998)   DOI   ScienceOn
21 Lee J. J., Kim, Y. M., Yin, S. Y., Park, H. D., Kang, M. H., Hong, J. T., and Lee, M. K., Aggravation of L-DOPA neurotoxicity by tetrahydropapaveroline in PC12 cells. Biochem. Pharmacol., 66, 1787-1795 (2003)   DOI   ScienceOn
22 Nicoletti, I., Migliorati, G., Pagliacci, M. C., Grignani, F., and Riccardi, C., A rapid simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods., 139, 271-279 (1991)   DOI   ScienceOn