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Stimulation of Cell Growth by Erythropoietin in RAW264.7 Cells: Association with AP-1 Activation  

Seong Seu-Run (College of Pharmacy, Chungbuk National University)
Lee Jae-Woong (College of Pharmacy, Chungbuk National University)
Lee Yong-Kyoung (College of Pharmacy, Chungbuk National University)
Kim Tae-Il (College of Pharmacy, Chungbuk National University)
Son Dong-Ju (College of Pharmacy, Chungbuk National University)
Moon Dong-Cheol (College of Pharmacy, Chungbuk National University)
Yun Young-Won (College of Veterninary Medicine, Chungbuk National University)
Yoon Do-Young (Korea Research Institute of Bioscience and Biotechnology)
Hong Jin-Tae (College of Pharmacy, Chungbuk National University)
Publication Information
Archives of Pharmacal Research / v.29, no.3, 2006 , pp. 218-223 More about this Journal
Abstract
Erythropoietin (EPO), a hematopoietic factor, is required for normal erythrocyte developments, but it has been demonstrated to have many other functions, and its receptor is localized in other tissues. In the present study, we investigated whether EPO can promote other cell proliferation and possible molecular mechanisms. EPO restored the inhibition of the RAW264.7 and PC12 cell growth by fetal bovine serum (FBS) withdrawal in a dose dependent manner, but not that of other cell types tested. The restoring effect of EPO was completed when the RAW264.7 cells were cultured in the medium containing as low as 3% of FBS, and 10 U/mL EPO could replace FBS. The restoring effect of EPO in the RAW264.7 cells was associated with the increased of c-Fos and c-Jun expression as well as AP-1 activation. These data demonstrate that EPO can stimulate RAW264. 7 cell as well as PC12 cell growth even when the cells were cultured without FBS or in the presence of small amounts of FBS in the medium, and this stimulating effect is associated with the activation of AP-1 transcription factor.
Keywords
EPO; Cell growth; AP-1; Erythropoietin;
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1 Adunyah, S. E., Ceesay, K. J., and Rivero, J. A., Regulation of jun expression and activation of AP-1 activity by erythropoietin. Biochem. Biophys. Res. Commun., 221, 213-218 (1996)   DOI   ScienceOn
2 Damen, J. E., Wakao, H., Miyajima, A., Krosl, J., Humphries, R. K., Cutler, R. L., and Krystal, G., Tyrosine 343 in the erythropoietin receptor positively regulates erythropoietininduced cell proliferation and Stat5 activation. EMBO J., 14, 5557-5568 (1995)
3 Jacobs-Helber, S. M., Penta, K., Sun, Z. H., Lawson, A., and Sawyer, S. T., Distinct signaling from stem cell factor and erythropoietin in HCD57 cells. J. Biol. Chem., 272, 6850-6853 (1997)   DOI   ScienceOn
4 Jelkmann, W., Molecular biology of erythropoietin. Intern. Med., 43, 649-659 (2004)   DOI
5 Sawyer, S. T., and Penta, K., Erythropoietin cell biology. Hematol. Oncol. Clin. North Am., 8, 895–911 (1994)
6 Sigounas, G., Steiner, M., and Anagnostou, A., Synergism of hemopoietic growth factors on endothelial cell proliferation. Angiology, 48, 141-147 (1997)   DOI   ScienceOn
7 Lee, S. M., Nguyen, T. H., Park, M. H., Kim, K. S., Cho, K. J., Moon, D. C., Kim, H. Y., Yoon, D. Y., and Hong, J. T., EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes. Biochem. Biophys. Res. Commun., 320, 1087-1095 (2004)   DOI   ScienceOn
8 Shaulian, E. and Karin, M., AP-1 in cell proliferation and survival. Oncogene, 20, 2390-2400 (2001)   DOI
9 Farrell, F. and Lee, A., The erythropoietin receptor and its expression in tumor cells and other tissues. Oncologist, 9, 18-30 (2004)   DOI   ScienceOn
10 Fisher, J. W., Erythropoietin: physiology and pharmacology update. Exp. Biol. Med., 228, 1-14 (2003)   DOI
11 Sugawa, M., Sakurai, Y., Ishikawa-Ieda, Y., Suzuki, H., and Asou, H., Effects of erythropoietin on glial cell development; oligodendrocyte maturation and astrocyte proliferation. Neurosci. Res., 44, 391-403 (2002)   DOI   ScienceOn
12 Sasaki, R., Masuda, S., and Nagao, M., Erythropoietin: multiple physiological functions and regulation of biosynthesis. Biosci. Biotechnol. Biochem., 64, 1775-1793 (2000)   DOI   ScienceOn
13 Vairano, M., Dello, Russo C., Pozzoli, G., Battaglia, A., Scambia, G., Tringali, G., Aloe-Spiriti, M. A., Preziosi, P., and Navarra, P., Erythropoietin exerts anti-apoptotic effects on rat microglial cells in vitro. Eur. J. Neurosci., 16, 584-592 (2002)   DOI   ScienceOn
14 Jacobs-Helber S. M., Wickrema, A., Birrer, M. J., and Sawyer, S. T., AP1 regulation of proliferation and initiation of apoptosis in erythropoietin-dependent erythroid cells. Mol. Cell. Biol., 18, 3699-3707 (1998)   DOI
15 Hess, J., Angel, P., and Schorpp-Kistner, M., AP-1 subunits: quarrel and harmony among siblings. J. Cell. Sci., 117, 5965- 5973 (2004)   DOI   ScienceOn
16 Patel, H. R. and Sytkowski, A. J., Erythropoietin activation of AP1 (Fos/Jun). Exp. Hematol., 23, 619–625 (1995)
17 Jung, K. M., Park, K. S., Oh, J. H., Jung, S. Y., Yang, K. H., Song, Y. S., Son, D. J., Park, Y. H., Yun, Y. P., Lee, M. K., Oh, K. W., and Hong, J. T., Activation of p38 mitogen-activated protein kinase and activator protein-1 during the promotion of neurite extension of PC-12 cells by 15-deoxy-delta12, 14- prostaglandin J2. Mol. Pharmacol., 63, 607-616 (2003)   DOI   ScienceOn
18 Liboi, E., Carroll, M., D'Andrea, A. D., and Mathey-Prevot, B., Erythropoietin receptor signals both proliferation and erythroid-specific differentiation. Proc. Natl. Acad. Sci. U S A., 90, 11351-11355 (1993)
19 Schutte, J., Minna, J. D., and Birrer, M. J., Deregulated expression of human c-jun transforms primary rat embryo cells in cooperation with an activated c-Ha-ras gene and transforms Rat-1a cells as a single gene. Proc. Natl. Acad. Sci. U.S.A., 86, 2257–2261 (1989)
20 Jelkmann, W. and Wagner K., Beneficial and ominous aspects of the pleiotropic action of erythropoietin. Ann. Hematol., 83, 673-686 (2004)   DOI
21 Wojchowski, D. M., and He, T. C., Signal transduction in the erythropoietin receptor system. Stem Cells, 11, 381-392 (1993)   DOI
22 Bergelson, S., Klingmuller, U., Socolovsky, M., Hsiao, J. G., and Lodish, H. F., Tyrosine residues within the intracellular domain of the erythropoietin receptor mediate activation of AP-1 transcription factors. J. Biol. Chem., 273, 2396-2401 (1998)   DOI   ScienceOn