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Anticoagulant 1,2,3,4,6-Pentagalloyl-$\beta$-D-Glucopyranose Isolated from Geranium (Pelargonium inquinans Ait)  

Ji Myeong-Sim (Department of Agricultural Chemistry and Institute of Agricultural Science and Technology, Chonnam National University)
Piao Xiang-Lan (College of Pharmacy, Seoul National University)
Jin Yu-Lan (Department of Plant Protection, Laiyang Agricultural College)
Park Ro Dong (Department of Agricultural Chemistry and Institute of Agricultural Science and Technology, Chonnam National University)
Publication Information
Archives of Pharmacal Research / v.28, no.9, 2005 , pp. 1037-1041 More about this Journal
Abstract
Geranium (Pelargonium inquinans Ait) leaves were extracted with $80\%$ MeOH, and partitioned into n-hexane, ethyl acetate, BuOH and $H_2O$ to isolate the anticoagulant principles. The EtOAc fraction was found to be the most active, and was further purified using silica and octadecylisilane column chromatography employing a bioassay-guided fractionation method. The active compound was isolated and identified as $1,2,3,4,6-pentagalloyl-\beta-D-glucopyranose$(PGG) (compound I). The isolated anticoagulant significantly prolonged the activated partial thrombin time (APTT) and thrombin time (TT) using normal human plasma. One microgram of $1,2,3,4,6-pentagalloyl-\beta-D-glucopyranose$ showed 0.063 heparin units in the APTT and 2.73 heparin units in the TT for anti-thrombosis. This is the first report of the isolation of PGG from geranium plants.
Keywords
Geranium; Pelargonium inquinans Ait; Anticoagulant activity; Activated partial thrombin time (APTT); Thrombin time (TT);
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