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Hepatoprotective Constituents of the Edible Brown Alga Ecklonia stolonifera on Tacrine-induced Cytotoxicity in Hep G2 Cells  

Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
An, Ren-Bo (College of Pharmacy, Yanbian University)
Yoon, Na-Young (Faculty of Food Science and Biotechnology, Pukyong National University)
Nam, Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyong National University)
Choi, Jae-Sue (Faculty of Food Science and Biotechnology, Pukyong National University)
Publication Information
Archives of Pharmacal Research / v.28, no.12, 2005 , pp. 1376-1380 More about this Journal
Abstract
In this study, ethanolic extracts from 18 seaweed variants were assessed for hepatoprotective activity against tacrine-induced cytotoxicity in Hep G2 cells. Only one of these, Ecklonia stolonifera Okamura (Laminariaceae), a member of the brown algae, exhibited promising hepatoprotective activity. Bioassay-guided fractionation of the active ethyl acetate (EtOAc) soluble fraction obtained from the ethanolic extract of E. stolonifera, resulted in the isolation of several phlorotannins [phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5)]. Compounds 2 and 4 were determined to protect Hep G2 cells against the cytotoxic effects of tacrine, with $EC_{50}$ values of 62.0 and 79.2 $\mu$g/mL, respectively. Silybin, a well characterized hepatoprotective agent, was used as a positive control, and exhibited an $EC_{50}$ value of 50.0 $\mu$g/mL. It has been suggested that the phlorotannins derived from marine brown algae might prove useful sources in the development of novel hepatoprotective agents.
Keywords
Ecklonia stolonifera; Hepatoprotective; Phlorotannins; Marine algae; Tacrine; Hep G2 cells;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 19  (Related Records In Web of Science)
Times Cited By SCOPUS : 17
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