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Spontaneous Release of Glycosylphosphatidylinositol (GPI)-anchored Renal Dipeptidase from Porcine Renal Proximal Tubules  

Park, Sung-Wook (Department of Pharmacy, College of Pharmacy)
Kang, Bok-Yun (Department of Pharmacy, College of Pharmacy)
Yoon, Hyun-Joong (Department of Biological Sciences, College of National Science, Chonnam National University)
Park, Eun-Mi (Department of Pharmacy, College of Pharmacy)
Choi, Kyong (Department of Biological Sciences, College of National Science, Chonnam National University)
Lee, Hwang-Hee Blaise (Department of Biological Sciences, College of National Science, Chonnam National University)
Hooper, Nigel M. (Proteolysis Research Group, School of Biochemistry and Molecular Biology, University of Leeds)
Park, Haeng-Soon (Department of Pharmacy, College of Pharmacy)
Publication Information
Archives of Pharmacal Research / v.25, no.1, 2002 , pp. 80-85 More about this Journal
Abstract
The incubation of porcine renal proximal tubules (PTs) resulted in the release of the Glycosylphosphatidylinositol (GPI)-anchored renal dipeptidase (RDPase, EC 3. 4. 13. 19) from the membrane after a lag period of approximately 6 hours. This spontaneous release of RDPase from the membrane was inhibited by antibiotics. When the incubation supernatant was added back to fresh PTs, both the antibiotic inhibition of RDPase release and the lag period disappeared. The released RDPase reacted with an anti-cross reacting determinant antibody indicating the presence of the Ins (1, 2-cyc)P. These results suggest that bacteria in the PTs, when incubated, grow find Secrete a phosphatidylinmsitol-specific phospholipase C (PIPLC). This enzyme then hydrolyses the GPI-anchored RDPase and is transferable. RDPase was purified following its release from the membrane by this simple and inexpensive method which may also be applied to other GPI-anchored proteins.
Keywords
Spontaneous release; glycosylphosphatidylinositol (GPI)-anchor; cross-reacting determinant (CRD); phospholipase C; antibiotics;
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