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A Protocol for High Frequency Plant Conversion from Somatic Embryos of Peanut (Arachis hypogaea L. cv. DRG-12)  

Rani A. Raja (Department of Plant Sciences, School of Life Sciences, University of Hyderabad)
Padmaja G. (Department of Plant Sciences, School of Life Sciences, University of Hyderabad)
Publication Information
Journal of Plant Biotechnology / v.7, no.3, 2005 , pp. 187-193 More about this Journal
Abstract
A protocol was developed for somatic embryogenesis with 100% induction rate from immature zygotic embryo axes of peanut (Arachis hypogaea L. cv. DRG-12) cultured on MS medium containing $18.09\;{\mu}M$ 2,4-D. The frequency of somatic embryogenesis (31.7%) as well as the number of somatic embryos induced per explant (6.6) decreased when the concentration of 2,4-D was increased to $72.4\;{\mu}M$. Morphologically abnormal somatic embryos were observed at a frequency of 43.3% on MS medium containing $72.4\;{\mu}M$ 2,4-D. Somatic embryos isolated from 30-day-old cultures of immature zygotic embryo axes exhibited precocious germination with varied responses when placed on MS basal medium with 3% sucrose. Maximum shoot induction (80.0%) was observed from somatic embryos isolated from 60-day-old cultures of immature zygotic embryo axes when placed as a clump rather than individually on MS medium supplemented with $26.63\;{\mu}M$ BA and $0.54\;{\mu}M$ NAA. Shoots developed from somatic embryos rooted with higher frequency (93.3%) on Blaydes' medium containing $5.4\;{\mu}M$ NAA.
Keywords
Immature zygotic embryo axes; peanut; somatic embryos; plant conversion;
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