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http://dx.doi.org/10.3839/jabc.2016.027

Endophytic Bacillus subtilis MJMP2 from Kimchi inhibits Xanthomonas oryzae pv. oryzae, the pathogen of Rice bacterial blight disease  

Cheng, Jinhua (Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University)
Jaiswal, Kumar Sagar (Center for Nutraceutical and Pharmaceutical Materials, Myongji University)
Yang, Seung Hwan (Center for Nutraceutical and Pharmaceutical Materials, Myongji University)
Suh, Joo-Won (Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University)
Publication Information
Journal of Applied Biological Chemistry / v.59, no.2, 2016 , pp. 149-154 More about this Journal
Abstract
An endophytic bacterial strain was isolated from kimchi, a Korean traditional fermented Brassica campestris and identified as Bacillus subtilis MJMP2 based on the 16S rRNA sequence. This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease, as well as activity against some other rice phytopathogenic fungi. The active compound was purified through size-exclusion chromatography and preparative High-performance liquid chromatography. The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A. Furthermore, a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay. The crude extract also enhanced the length of roots of Arabidopsis thaliana. These results suggest that the strain Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease.
Keywords
Bacillus subtilis; Bacterial blight disease; Biocontrol; Iturin A; Xanthomonas oryzae;
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1 Arrebola E, Jacobs R, Korsten L (2010) Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens. J Appl Microbiol 108: 386-395   DOI
2 Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl Environ Microbiol 62: 4081-4085
3 Duitman EH, Hamoen LW, Rembold R, Venema G, Seitz H, Saenger W, Bernhard F, Reinhardt R, Schmidt M, Ullrich C, Stein T, Leenders F, Vater J (1999) The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase. Proc Natl Acad Sci 96: 13294-13299   DOI
4 Emmert EA, Handelsman J (1999) Biocontrol of plant disease: a (Gram) positive perspective. FEMS Microbiol Lett 171: 1-9   DOI
5 Ezuka A, Kaku H (2000) A historical review of bacterial blight of rice. Bull Natl Inst Agrobiol Resour (Japan) 1553-1554.
6 Hoitink H, Boehm M (1999) Biocontrol within the context of soil microbial communities: a substrate-dependent phenomenon. Annu Rev Phytopathol 37: 427-446   DOI
7 Hsieh FC, Lin TC, Meng M, Kao SS (2008) Comparing methods for identifying Bacillus strains capable of producing the antifungal lipopeptide iturin A. Curr Microbiol 56: 1-5   DOI
8 Kim PI, Ryu J, Kim YH and Chi YT (2010) Production of biosurfactant lipopeptides iturin A, fengycin, and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J Microbiol Biotechnol 20: 138-145
9 Kloepper JW, Ryu CM, Zhang S (2004) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94: 1259-1266   DOI
10 Kowall M, Vater J, Kluge B, Stein T, Franke P, Ziessow D (1998) Separation and characterization of surfactin isoforms produced by Bacillus subtilis OKB 105. J Colloid Interface Sci 204: 1-8   DOI
11 Li SB, Xu SR, Zhang RN, Liu Y, Zhou RC (2015) The antibiosis action and rice induced resistance, mediated by a lipopeptide from Bacillus amyloliquefaciens B014, in controlling rice disease caused by Xanthomonas oryzae pv. Oryzae. J Microbiol Biotechnol doi: 10.4014/jmb.1510.10072
12 Maget-Dana R, Peypoux F (1994) Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties. Toxicology 87: 151-174   DOI
13 Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual vol 545. Cold Spring Harbor Laboratory. Cold Spring Harbor: NY
14 Moyne A-L, Cleveland TE, Tuzun S (2004) Molecular characterization and analysis of the operon encoding the antifungal lipopeptide bacillomycin D. FEMS Microbiol Lett 234: 43-9   DOI
15 Nagendran K, Karthikeyan G, Peeran MF, Raveendran M, Prabakar K, Raguchander T (2013) Management of bacterial leaf blight disease in rice with endophytic bacteria. World Appl Sci J 28: 2229-2241
16 Ohno A, Ano T, Shoda M (1993) Production of the antifungal peptide antibiotic, iturin by Bacillus subtilis NB22 in solid state fermentation. J Ferment Bioeng 75: 23-27   DOI
17 Paul NC, Deng JX, Sang HK, Choi YP, Yu SH (2012) Distribution and antifungal activity of endophytic fungi in different growth stages of chili pepper (Capsicum annuum L.) in Korea. Plant Patho J 28: 10-19   DOI
18 Thimon L, Peypoux F, Wallach J, Michel G (1995) Effect of the lipopeptide antibiotic, iturin A, on morphology and membrane ultrastructure of yeast cells. FEMS Microbiol Lett 128: 101-106   DOI
19 Schallmey M, Singh A, Ward OP (2004) Developments in the use of Bacillus species for industrial production. Can J Microbiol 50: 1-17   DOI
20 Schneider J, Taraz K, Budzikiewicz H, Deleu M, Thonart P, Jacques P (1999) The structure of two fengycins from Bacillus subtilis S499. Z Naturforsch C 54: 859-866
21 Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697-703   DOI
22 Weller DM, Raaijmakers JM, Gardener BBM, Thomashow LS (2002) Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu Rev Phytopathol 40: 309-348   DOI
23 Whipps JM (1997) Developments in the Biological Control of Soil-borne Plant Pathogens. Adv Bot Res 26: 1-134   DOI
24 Yoshida S, Hiradate S, Tsukamoto T, Hatakeda K, Shirata A (2001) Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves Phytopathology 91: 181-187   DOI
25 Yu G, Sinclair J, Hartman G, Bertagnolli B (2002) Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biol Biochem 34: 955-963   DOI