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http://dx.doi.org/10.5483/BMBRep.2015.48.1.213

Autocrine stimulation of IL-10 is critical to the enrichment of IL-10-producing CD40hiCD5+ regulatory B cells in vitro and in vivo  

Kim, Hyuk Soon (School of Medicine, Konkuk University)
Lee, Jun Ho (College of Medicine, Korea University)
Han, Hee Dong (School of Medicine, Konkuk University)
Kim, A-Ram (School of Medicine, Konkuk University)
Nam, Seung Taek (School of Medicine, Konkuk University)
Kim, Hyun Woo (School of Medicine, Konkuk University)
Park, Young Hwan (School of Medicine, Konkuk University)
Lee, Dajeong (School of Medicine, Konkuk University)
Lee, Min Bum (School of Medicine, Konkuk University)
Park, Yeong Min (School of Medicine, Konkuk University)
Kim, Hyung Sik (College of Pharmacy, Sungkyunkwan University)
Kim, Young Mi (College of Pharmacy, Duksung Women's University)
You, Ji Chang (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Choi, Wahn Soo (School of Medicine, Konkuk University)
Publication Information
BMB Reports / v.48, no.1, 2015 , pp. 54-59 More about this Journal
Abstract
IL-10-producing B (Breg) cells regulate various immune responses. However, their phenotype remains unclear. CD40 expression was significantly increased in B cells by LPS, and the Breg cells were also enriched in $CD40^{hi}CD5^+$ B cells. Furthermore, CD40 expression on Breg cells was increased by IL-10, CD40 ligand, and B cell-activating factor, suggesting that $CD40^{hi}$ is a common phenotype of Breg cells. LPS-induced CD40 expression was largely suppressed by an anti-IL-10 receptor antibody and in IL-$10^{-/-}CD5^+CD19^+$ B cells. The autocrine effect of IL-10 on the CD40 expression was largely suppressed by an inhibitor of JAK/STAT3. In vivo, the LPS treatment increased the population of $CD40^{hi}CD5^+$ Breg cells in mice. However, the population of $CD40^{hi}CD5^+$ B cells was minimal in IL-$10^{-/-}$ mice by LPS. Altogether, our findings show that Breg cells are largely enriched in $CD40^{hi}CD5^+$ B cells and the autocrine effect of IL-10 is critical to the formation of $CD40^{hi}CD5^+$ Breg cells.
Keywords
Autocrine; CD5; CD40; Interleukin-10; Regulatory B cells;
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