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http://dx.doi.org/10.5483/BMBRep.2011.44.9.590

Production of methionine γ- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene  

Kahraman, Huseyin (Department of Biology, Faculty of Art and Science, Inonu University)
Aytan, Emel (Department of Biology, Faculty of Art and Science, Inonu University)
Kurt, Ash Giray (Department of Biology, Faculty of Art and Science, Inonu University)
Publication Information
BMB Reports / v.44, no.9, 2011 , pp. 590-594 More about this Journal
Abstract
The production of antileukemic enzyme methionine ${\gamma}$-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine ${\gamma}$- liyase production. Methionine ${\gamma}$- liyase production by Citrobacter freundii and its $vgb^-$ and $vgb^+$ bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and $30^{\circ}C$ in a time-course manner. The $vgb^+$ and especially the carbon type had a dramatic effect on methionine ${\gamma}$- liyase production. The $vgb^+$ strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and $vgb^-$ strain, respectively.
Keywords
Antitumour efficacy; Citrobacter freundii; Methionine ${\gamma}$-lyase; Vitreoscilla hemoglobin;
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1 Yang, J., Webster, D. A. and Stark, B. C. (2005) ArcA Works with Fnr as a positive regulator of Vitreoscilla (bacterial) hemoglobin gene expression in Escherichia coli. Microbiol. Res. 160, 405-415.   DOI   ScienceOn
2 Kurt, A. G., Aytan, E., Ozer, U., Ates, B. and Geckil, H. (2009) Production of L- DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene. Biotechnol. J. 4, 1077-1088.   DOI   ScienceOn
3 Tanaka, H., Imahara, H., Esaki, N. and Soda, K. (1980) Selective determination of L-methionine and L-cysteine with bacterial L-methionine $\gamma$-lyase and anti-tumor activity of the enzyme. J. Appl. Biochem. 2, 439-444.
4 Soda, K. (1968) Microdetermination of D-amino acids and D-amino acid oxidase activity with 3-methyl-2-benzothiazolone hydrazone hydrochloride. Anal. Biochem. 25, 228-235.   DOI   ScienceOn
5 Coombs, G. H. and Mottram, J. C. (2001) Trifluoromethionine, a prodrug designed against methionine $\gamma$-lyase-containing pathogens, has efficacy in vitro and in vivo against Trichomonas vaginalis. Antimicrob. Agents. Chem. 45, 1743-1745.   DOI   ScienceOn
6 Kokkinakis, D. M., Schold, S. C., Hori, H. and Nobori, T. (1997) Effect of long-term depletion of plasma methionine on the growth and survival of human brain tumor xenografts in athmic mice. Nutr. Cancer, 29, 195-204.   DOI
7 Wang, J., Chang, S., Chen, Y. and Luh, K. J. (2000) Comparison of antimicrobial susceptibility of Citrobacter freundii isolates in two different time periods. Microbiol. Immunol. Infect. 33, 258-262.
8 Takakura, T., Takimoto, A., Notsu, Y., Yoshida, H., Ito, T., Nagatome, H., Ohno, M., Kobayashi, Y., Yoshioka, T., Inagaki, K., Yagi, S., Hoffman, R. M. and Esaki, N. (2006) Physicochemical and pharmacokinetic characterization of highly potent recombinant L-methionine $\gamma$-lyase conjugated with polyethylene glycol as an antitumor agent. Cancer Res. 66, 2807-2814.   DOI   ScienceOn
9 Lishko, V. K., Lishko, O. V. and Hoffman, R. M. (1993) Depletion of serum methionine by methioninase in mice. Anticancer Res. 13, 1465-1468.
10 Borenshtein, D. and Schauer, D. B. (2006) The genus Citrobacter. Prokaryotes 6, 90-98.
11 Geckil, H., Gencer, S., Kahraman, H. and Erenler, S. O. (2003) Genetic engineering of Enterobacter aerogenes with Vitreoscilla hemoglobin gene: cell growth, survival, and antioxidant enzyme status under oxidative stress. Res. Microbiol. 154, 425-431.   DOI   ScienceOn
12 Geckil, H., Stark, B. C. and Webster, D. A. (2001) Cell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently affected by the genetically engineered Vitreoscilla hemoglobin gene. J. Biotechnol. 85, 57-66.   DOI   ScienceOn
13 Takakura, T., Mitsushima, K., Yagi, S., Inagaki, K., Tanaka, H., Esaki, N., Soda, K. and Takimotoa, A. (2004) Assay method for antitumor L-methionine $\gamma$-lyase: comprehensive kinetic analysis of the complex reaction with L-methionine. Anal. Biochem. 327, 233-240.   DOI   ScienceOn
14 Weimer, B., Seefeldt, K. and Dias, B. (1999) Sulfur metabolism in bacteria associated with cheese. Anton. Leeuw. 76, 247-261.   DOI   ScienceOn
15 Manukhov, I. V., Mamaeva, D. V., Rastorguev, S. M., Faleev, N. G., Morozova, E. A., Demidkina, T. V. and Zavilgelsky, G. B. (2005) A gene encoding L-methionine $\gamma$-lyase is present in Enterobacteriaceae family genomes: Identification and characterization of Citrobacter freundii L-methionine $\gamma$-lyase. J. Bacteriol. 187, 3889-3893.   DOI   ScienceOn
16 Manukhov, I. V., Mamaeva, D. V., Morozova, E. A., Rastorguev, S. M., Faleev, N. G., Demidkina, T. V. and Zavilgelsky, G. B. (2006) L-Methionine $\gamma$-lyase from Citrobacter freundii: cloning of the gene and kinetic parameters of the enzyme. Biochem. (Moscow) 71, 361-369.   DOI
17 Nikulin, A., Revtovich, S., Morozova, E., Nevskaya, N., Nikonov, S., Garber, M. and Demidkina, T. (2008) Highresolution structure of methionine $\gamma$-lyase from Citrobacter freundii. Acta Cryst. 64, 211-218.
18 Kudou, D., Misaki, S., Yamashita, M., Tamura, T., Takakura, T., Yoshioka, T., Yagi, S., Hoffman, R. M., Takimoto, A., Esaki, N. and Inagaki, K. (2007) Structure of the antitumour enzyme L-methionine γ-lyase from Pseudomonas putida at 1.8$A^{circ}$ Resolution. J. Biochem. 141, 535-544.   DOI   ScienceOn
19 Mamaeva, D. V., Morozova, E. A., Nikulin, A. D., Revtovich, S. V., Nikonov, S. V., Garberb, M. B. and Demidkinaa, T. V. (2005) Structure of Citrobacter freundii L-methionine $\gamma$-lyase. Acta Cryst. Section F 61, 546-549.   DOI   ScienceOn
20 Yoshimura, M., Nakano, Y. and Koga, T. (2002) L-Methionine- $\gamma$-lyase, as a target to inhibit malodorous bacterial growth by trifluoromethionine. Biochem. Bioph. Res. Co. 292, 964-968.   DOI   ScienceOn
21 Takakura, T., Ito, T., Yagi, S., Notsu, Y., Itakura, T., Nakamura, T., Inagaki, K., Esaki, N., Hoffman, R. M. and Takimoto, A. (2006) High-level expression and bulk crystallization of recombinant L-methionine $\gamma$-lyase, an anticancer agent. Appl. Microbiol. Biotechnol. 70, 183-192.   DOI
22 Geckil, H., Gencer, S., Ates, B., Ozer, U., Uckun, M. and Yilmaz, I. (2006) Effect of Vitreoscilla hemoglobin on production of a chemotherapeutic enzyme, L-asparaginase, by Pseudomonas aeruginosa. Biotechnol. J. 1, 203-208.   DOI   ScienceOn