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http://dx.doi.org/10.5483/BMBRep.2011.44.9.566

Altered PLCβ-1 expression in the gerbil hippocampal complex following spontaneous seizure  

Lee, Saet-Byeol (Department of Anatomy, College of Medicine, Soonchunhyang University)
Oh, Yun-Jung (Department of Anatomy, College of Medicine, Soonchunhyang University)
Chung, Jae-Kwang (Department of Anatomy, College of Medicine, Soonchunhyang University)
Jeong, Ji-Heon (Department of Anatomy, College of Medicine, Soonchunhyang University)
Lee, Sang-Duk (Department of Physical Education, Hallym University)
Park, Dae-Kyoon (Department of Anatomy, College of Medicine, Soonchunhyang University)
Park, Kyung-Ho (Department of Anatomy, College of Medicine, Soonchunhyang University)
Ko, Jeong-Sik (Department of Anatomy, College of Medicine, Soonchunhyang University)
Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University)
Publication Information
BMB Reports / v.44, no.9, 2011 , pp. 566-571 More about this Journal
Abstract
Although the phospholipase C (PLC)${\beta}$-1 isoform is associated with spontaneous seizure and distinctively expressed in the telencephalon, the distribution of PLC${\beta}$-1 expression in the epileptic gerbil hippocampus remains controversial. Therefore, we determined whether PLC${\beta}$-1 is associated with spontaneous seizure in an animal model of genetic epilepsy. In the present study, PLC${\beta}$-1 immunoreactivity was down-regulated in seizure-sensitive (SS) gerbils more than in seizure-resistant (SR) gerbils. The expression of PLC${\beta}$-1 within calretinin (CR)-positive neurons was rarely detected within the dentate hilar region of SS gerbils. PLC${\beta}$-1 immunoreactivity in the hippocampus was significantly elevated as compared to that in pre-seizure SS gerbil 3 h post-ictal. These findings suggest that alterations in PLC${\beta}$-1 immunoreactivity in the SS gerbil hippocampus may be closely related to the epileptic state of the gerbil brain and transiently elevated PLC${\beta}$-1 protein levels following seizure episodes. Such alterations may be compensatory responses in the SS gerbil hippocampus.
Keywords
Epilepsy; Gerbil; Hippocampus; Immunohistochemistry; Phospholipase C (PLC);
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