Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient Mutants |
Sirikhachornkit, Anchalee
(Microalgal Molecular Genetics and Functional Genomics Special Research Unit, Department of Genetics, Faculty of Science, Kasetsart University)
Vuttipongchaikij, Supachai (Microalgal Molecular Genetics and Functional Genomics Special Research Unit, Department of Genetics, Faculty of Science, Kasetsart University) Suttangkakul, Anongpat (Microalgal Molecular Genetics and Functional Genomics Special Research Unit, Department of Genetics, Faculty of Science, Kasetsart University) Yokthongwattana, Kittisak (Department of Biochemistry, Faculty of Science, Mahidol University) Juntawong, Piyada (Microalgal Molecular Genetics and Functional Genomics Special Research Unit, Department of Genetics, Faculty of Science, Kasetsart University) Pokethitiyook, Prayad (Department of Biology, Faculty of Science, Mahidol University) Kangvansaichol, Kunn (PTT Research and Technology Institute, PTT Public Company Limited) Meetam, Metha (Department of Biology, Faculty of Science, Mahidol University) |
1 | Ball S, Marianne T, Dirick L, Fresnoy M, Delrue B, Decq A. 1991. A Chlamydomonas reinhardtii low-starch mutant is defective for 3-phosphoglycerate activation and orthophosphate inhibition of ADP-glucose pyrophosphorylase. Planta 185: 17-26. DOI |
2 | Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911-917. DOI |
3 | Blaby IK, Glaesener AG, Mettler T, Fitz-Gibbon S, Gallaher SD, Liu B, et al. 2013. Systems-level analysis of nitrogen starvation-induced modifications of carbon metabolism in a Chlamydomonas reinhardtii starchless mutant. Plant Cell 25: 4305-4323. DOI |
4 | Bölling C, Fiehn O. 2005. Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation. Plant Physiol. 139: 1995-2005. DOI |
5 | Brennan L, Owende P. 2010. Biofuels from microalgae - A review of technologies for production, processing, and extractions of biofuels and co-products. Renew. Sust. Energ. Rev. 14: 557-577. DOI |
6 | Breuer G, De Jaeger L, Artus VPG, Martens DE, Springer J, Draaisma RB, et al. 2014. Superior triacylglycerol (TAG) accumulation in starchless mutants of Scenedesmus obliquus: (II) evaluation of TAG yield and productivity in controlled photobioreactors. Biotechnol. Biofuels 7: 70. DOI |
7 | Breuer G, Evers WA, de Vree JH, Kleinegris DM, Martens DE, Wijffels RH, Lamers PP. 2013. Analysis of fatty acid content and composition in microalgae. J. Vis. Exp. 80: 50628. |
8 | Breuer G, Lamers PP, Martens DE, Draaisma RB, Wijffels RH. 2012. The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresour. Technol. 124: 217-226. DOI |
9 | Davidi L, Shimoni E, Khozin-Goldberg I, Zamir A, Pick U. 2014. Origin of β-carotene-rich plastoglobuli in Dunaliella bardawil. Plant Physiol. 164: 2139-2156. DOI |
10 | De Jaeger L, Verbeek REM, Draaisma RB, Martens DE, Springer J, Eggink G, Wijffels RH. 2014. Superior triacylglycerol(TAG) accumulation in starchless mutants of Scenedesmus obliquus: (I) mutant generation and characterization. Biotechnol. Biofuels 7: 69. DOI |
11 | Deng XD, Cai JJ, Fei XW. 2013. Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii. BMC Biochem. 14: 38. DOI |
12 | Dunnahay TG, Jarvis EE, Roessler PG. 1995. Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila. J. Phycol. 31: 1004-1012. DOI |
13 | Fernandes B, Teixeira J, Dragone G, Vicente AA, Kawano S, Bišová K, et al. 2013. Relationship between starch and lipid accumulation induced by nutrient depletion and replenishment in the m icroalga Parachlorella kessleri. Bioresour. Technol. 144: 268-74. DOI |
14 | La Russa M, Bogen C, Uhmeyer A, Doebbe A, Fillippone E, Kruse O, Mussgnug JH. 2012. Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii. J. Biotechnol. 162: 13-20. DOI |
15 | Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A. 2008. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 54: 621-639. DOI |
16 | Kim S-H, Liu K-H, Lee S-Y, Hong S-J, Cho B-K, Lee H, et al. 2013. Effects of light intensity and nitrogen starvation on glycerolipid, glycerophospholipid, and carotenoid composition in Dunaliella tertiolecta culture. PLoS One 8: e72415. DOI |
17 | Krishnan A, Kumaraswamy GK, Vinyard DJ, Gu H, Ananyev G, Posewitz MC, Dismukes GC. 2015. Metabolic and photosynthetic consequences of blocking starch biosynthesis in the green alga Chlamydomonas reinhardtii sta6 mutant. Plant J. 81: 947-960. DOI |
18 | Lamers PP, Janssen M, De Vos RCH, Bino RJ, Wijffels RH. 2012. Carotenoid and fatty acid metabolism in nitrogenstarved Dunaliella salina, a unicellular green microalga. J. Biotechnol. 162: 21-27. DOI |
19 | Lee S -Y, Kim S-H, Hyun S -H, Suh HW, Hong S-J, Cho B-K, et al. 2014. Fatty acids and global metabolites profiling of Dunaliella tertiolecta by shifting culture conditions to nitrate deficiency and high light at different growth phases. Process Biochem. 49: 996-1004. DOI |
20 | Li T, Gargouri M, Feng J, Park JJ, Gao D, Miao C, et al. 2015. Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana. Bioresour. Technol. 180: 250-257. DOI |
21 | Lichtenthaler H. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 148: 350-382. |
22 | Li X, Moellering ER, Liu B, Johny C, Fedewa M, Sears BB, et al. 2012. A galactoglycerolipid lipase is required for triacylglycerol accumulation and survival following nitrogen deprivation in Chlamydomonas reinhardtii. Plant Cell 24: 4670-4686. DOI |
23 | Li Y, Han D, Hu G, Dauvillee D, Sommerfeld M, Ball S, Hu Q. 2010. Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol. Metab. Eng. 12: 387-391. DOI |
24 | Li Y, Han D, Hu G, Sommerfeld M, Hu Q. 2010. Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii. Biotechnol. Bioeng. 107: 258-268. DOI |
25 | Liu B, Benning C. 2013. Lipid metabolism in microalgae distinguishes itself. Curr. Opin. Biotechnol. 24: 300-309. DOI |
26 | Posewitz MC, Smolinski S, Kanakagiri S, Melis A, Seibert M, Ghirardi ML. 2010. Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii. Plant Cell 16: 2151-2163. DOI |
27 | Rabbani S, Beyer P, Lintig J, Hugueney P, Kleinig H. 1998. Induced β-carotene synthesis driven by triacylglycerol deposition in the unicellular alga Dunaliella bardawil. Plant Physiol. 116: 1239-1248. DOI |
28 | Rodolfi L, Chitin Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR. 2008. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol. Bioeng. 102: 100-112. DOI |
29 | Schneider CA, Rasband WS, Eliceiri KW. 2012. NIH image to ImageJ: 25 years of image analysis. Nat. Methods 9: 671-675. DOI |
30 | Scott SA, Davey MP, Dennis JS, Horst I, Howe CJ, Lea-Smith DJ, Smith AG. 2010. Biodiesel from algae: challenges and prospects. Curr. Opin. Biotechnol. 21: 277-286. DOI |
31 | Shin HS, Hong S-J, Kim H, Yoo C, Lee H, Choi H-K, et al. 2015. Elucidation of the growth delimitation of Dunaliella tertiolecta under nitrogen stress by integrating transcriptome and peptidome analysis. Bioresour. Techol. 194: 57-66. DOI |
32 | Siaut M, Cuiné S, Cagnon C, Fessler B, Nguyen M, Carrier P, et al. 2011. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnol. 11: 7. DOI |
33 | Spolore P, Joannis-Cassan C, Duran E, Isambert A. 2006. Commercial applications of microalgae. J. Biosci. Bioeng. 101: 87-96. DOI |
34 | Tafreshi AH, Shariati M. 2009. Dunaliella biotechnology: methods and applications. J. Appl. Microbiol. 107: 14-35. DOI |
35 | Tang H, Abunasser N, Garcia MED, Chen M, Ng KYS, Salley OS. 2011. Potential of microalgae oil from Dunaliella tertiolecta as a feedstock for biodiesel. Appl. Energy 88: 3324-3330. DOI |
36 | Thaipratum R, Melis A, Svasti J, Yokthongwattana K. 2009. Analysis of non-photochemical energy dissipating processes in wild type Dunaliella salina (green algae) and in zea1, a mutant constitutively accumulating zeaxanthin. J. Plant Res. 122: 465-476. DOI |
37 | Trentacoste EM, Shrestha RP, Smith SR, Glé C, Hartmann AC, Hildebrand M, Gerwick WH. 2013. Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth. Proc. Natl. Acad. Sci. USA 110: 19748-19753. DOI |
38 | Vorst P, Baard RL, Mur LR, Koprthals HJ, Van Den Ende H. 1994. Effect of growth arrest on carotene accumulation and photosynthesis in Dunaliella. Microbiology 140: 1411-1417. DOI |
39 | Uriarte I, Farías A, Hawkins AJS, Bayne BL. 1993. Cell characteristics and biochemical composition of Dunaliella primolecta Butcher conditioned at different concentrations of dissolved nitrogen. J. Appl. Phycol. 5: 447-453. DOI |
40 | Vonlanthen S, Dauvillée D, Purton S. 2015. Evaluation of novel starch-deficient mutants of Chlorella sorokiniana for hyper-accumulation of lipids. Algal Res. 12: 109-118. DOI |
41 | Zabawinski C, Van Den Koornhuyse N, D´Hulst C, Schlichting R, Giersch C, Delrue B, et al. 2001. Starchless mutants of Chlamydomonas reinhardtii lack the small subunit of a heterotetrameric ADP-glucose pyrophosphorylase. J. Bacteriol. 183: 1069-1077. DOI |
42 | Zhang Y-M, Chen H, He C-L, Wang Q. 2013. Nitrogen starvation induced oxidative stress in an oil-producing green alga Chlorella sorokiniana C3. PLoS One 8: e69225. DOI |
43 | Zhu S, Huang W, Wang Z, Xu J, Yuan Z. 2014. Metabolic changes of starch and lipid triggered by nitrogen starvation in the microalga Chlorella zofingiensis. Bioresour. Technol. 152: 292-298. DOI |