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http://dx.doi.org/10.4014/jmb.1505.05015

Characterization of Lactobacillus fermentum PL9988 Isolated from Healthy Elderly Korean in a Longevity Village  

Park, Jong-Su (R&D Center, Namyang Dairy Products Co.)
Shin, Eunju (Culture Collection of Antimicrobial Resistant Microbes (CCARM), Department of Horticulture, Biotechnology and Landscape Architecture,Seoul Women's University)
Hong, Hyunjin (Culture Collection of Antimicrobial Resistant Microbes (CCARM), Department of Horticulture, Biotechnology and Landscape Architecture,Seoul Women's University)
Shin, Hyun-Jung (R&D Center, Namyang Dairy Products Co.)
Cho, Young-Hoon (R&D Center, Namyang Dairy Products Co.)
Ahn, Ki-Hyun (Hyanglim Co.)
Paek, Kyungsoo (Department of Chemistry, Soongsil University)
Lee, Yeonhee (Culture Collection of Antimicrobial Resistant Microbes (CCARM), Department of Horticulture, Biotechnology and Landscape Architecture,Seoul Women's University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.9, 2015 , pp. 1510-1518 More about this Journal
Abstract
In this work, we wanted to develop a probiotic from famous longevity villages in Korea. We visited eight longevity villages in Korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. Isolated colonies that appeared on MRS agar containing bromophenol blue were identified by means of 16S rRNA sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 species). Lactobacillus fermentum was the most frequently found species. Eight isolates were selected on the basis of their ability to inhibit the growth of six intestinal pathogens (Escherichia coli O157:H7, Salmonella enterica subsp. enterica Typhimurium, Salmonella enterica subsp. enterica Enteritidis, Enterococcus faecalis, Staphylococcus aureus, and Listeria monocytogenes) and their susceptibility to 15 antimicrobial agents. Among these eight isolates, four Lactobacillus fermentum isolates were found not to produce any harmful enzymes or metabolites. Among them, Lactobacillus fermentum isolate no. 24 showed the strongest binding to intestinal epithelial cells, the highest immune-enhancing activity, anti-inflammation activity, and anti-oxidation activity as well as the highest survival rates in the presence of artificial gastric juice and bile solution. This isolate, designated Lactobacillus fermentum PL9988, has all the characteristics for a good probiotic.
Keywords
Lactic-acid-producing bacteria; probiotic; Lactobacillus fermentum; longevity;
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1 Sanz Y, Olivares M, Moya-Pérez Á, Agostoni C. 2015. Understanding the role of gut microbiome in metabolic disease risk. Pediatr. Res. 77: 236-244.   DOI
2 Song YL, Kato N, Matsumiya Y, Liu C, Kato H, Watanabe K. 1999. Identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from Japanese women and newborn infants. J. Clin. Microbiol. 37: 3062-3064.
3 Teuber M, Meile L, Schwarz F. 1999. Acquired antibiotic resistance in lactic acid bacteria from food. Antonie Van Leeuwenhoek 76: 115-137.   DOI
4 Thumu SC, Halami PM. 2012. Presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of Indian origin. Antonie Van Leeuwenhoek 102: 541-551.   DOI
5 Tuomola E, Crittenden R, Playne M, Isolauri E, Salminen S. 2001. Quality assurance criteria for probiotic bacteria. Am. J. Clin. Nutr. 73: 393S-398S.   DOI
6 Warren HS, Fitting C, Hoff E, Adib-Conquy M, Beasley-Topliffe L, Tesini B, et al. 2010. Resilience to bacterial infection: difference between species could be due to proteins in serum. J. Infect. Dis. 201: 223-232.   DOI
7 Wikler M, Clinical and Laboratory Standards Institute. 2007. Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement. Clinical and Laboratory Standards Institute, Wayne, PA.
8 Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, et al. 2012. Human gut microbiome viewed across age and geography. Nature 486: 222-227.
9 Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, et al. 2009. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW264.7 macrophages. J. Ethnopharmacol. 125: 286-290.   DOI
10 Mathara JM, Schillinger U, Guigas C, Franz C, Kutima PM, Mbugua SK, et al. 2008. Functional characteristics of Lactobacillus spp. from traditional Maasai fermented milk products in Kenya. Int. J. Food Microbiol. 126: 57-64.   DOI
11 Mathur S, Singh R. 2005. Antibiotic resistance in food lactic acid bacteria — a review. Int. J. Food Microbiol. 105: 281-295.   DOI
12 Million M, Lagier JC, Yahav D, Paul M. 2013. Gut bacterial microbiota and obesity. Clin. Microbiol. Infect. 19: 305-313.   DOI
13 Nam H, Ha M, Bae O, Lee Y. 2002. Effect of Weissella confusa strain PL9001 on the adherence and growth of Helicobacter pylori. Appl. Environ. Microbiol. 9: 4642-4645.   DOI
14 Park JH, Lee Y, Moon E, Seok SH, Baek MW, Lee HY, et al. 2005. Safety assessment of Lactobacillus fermentum PL9005, a potential probiotic lactic acid bacterium, in mice. J. Microbiol. Biotechnol. 15: 603-608.
15 Park JH, Lee Y, Moon E, Seok SH, Cho SA, Baek MW, et al. 2005. Immunoenhancing effects of a new probiotic strain, Lactobacillus fermentum PL9005. J. Food Prot. 68: 571-576.   DOI
16 Round JL, Mazmanian S. 2009. The gut microbiota shapes intestinal immune responses during health and disease. Nat. Rev. Immunol. 9: 313-323.   DOI
17 Kullisaar T, Zilmer M, Mikelsaar M, Vihalemm T, Annuk H, Kairane C, Kilk A. 2002. Two antioxidative lactobacilli strains as promising probiotics. Int. J. Food Microbiol. 72: 215-224.   DOI
18 Sanders ME, Akkermans LM, Haller D, Hammerman C, Heimbach J, Hörmannsperger G, et al. 2010. Safety assessment of probiotics for human use. Gut Microbes 1: 164-185.   DOI
19 Satokari R, Mattila E, Kainulainen V, Arkkila PE. 2015. Simple faecal preparation and efficacy of frozen inoculum in faecal microbiota transplantation for recurrent Clostridium difficile infection - an observational cohort study. Alimen. Pharmacol. Ther. 41: 46-53.   DOI
20 Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62: 716-721.   DOI
21 Lee HM, Lee Y. 2008. A differential medium for lactic acidproducing bacteria in a mixed culture. Lett. Appl. Microbiol. 46: 676-681.   DOI
22 Lee HS, Han SY, Bae EA, Huh CS, Ahn YT, Lee JH, Kim DH. 2008. Lactic acid bacteria inhibit proinflammatory cytokine expression and bacterial glycosaminoglycan degradation activity in dextran sulfate sodium-induced colitic mice. Int. Immunopharmacol. 8: 574-580.   DOI
23 Lee HY, Park JH, Seok SH, Baek MW, Kim DJ, Lee KE, et al. 2006. Human originated bacteria, Lactobacillus rhamnosus PL60, produce conjugated linoleic acid and show antiobesity effects in diet-induced obese mice. Biochim. Biophys. Acta 1761: 736-744.   DOI
24 Lee K, Paek K, Lee HY, Park JH, Lee Y. 2007. Antiobesity effect of trans-10, cis-12-conjugated linoleic acid-producing Lactobacillus plantarum PL62 on diet-induced obese mice. J. Appl. Microbiol. 103: 1140-1146.   DOI
25 Huys G, D’Haene K, Swings J. 2006. Genetic basis of tetracycline and minocycline resistance in potentially probiotic Lactobacillus plantarum strain CCUG 43738. Antimicrob. Agents Chemother. 50: 1550-1551.   DOI
26 Lee Y. 2005. Characterization of Weissella kimchi PL9023 as a potential probiotic for women. FEMS Microbiol. Lett. 250: 157-162.   DOI
27 Lourens-Hattingh A, Viljoen BC. Yogurt as probiotic carrier food. 2001. Int. Dairy J. 11: 1-17.   DOI
28 Mastromarino A, Reddy BS, Wynder EL. 1976. Metabolic epidemiology of colon cancer: enzymic activity of fecal flora. Am. J. Clin. Nutr. 29: 1455-1460.   DOI
29 International Standard Organization. 2010. Milk and milk products — Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB). ISO 10932:2010 (IDF 223:2010). Geneva, Switzerland.
30 Isenberg HD. 1992. Gelatin liquefaction test, pp. 1.19.42-1.19.43. Clinical Microbiology Procedures Handbook, Vol. 1. American Society of Microbiology, Washington, DC.
31 Isenberg HD. 1992. Hemolysis on sheep blood agar, pp. 1.20.16-1.20.17. Clinical Microbiology Procedures Handbook. Vol. 1. American Society of Microbiology, Washington D.C.
32 Isenberg HD. 1992. Indole test, pp. 1.19.13-1.19.15. Clinical Microbiology Procedures Handbook, Vol. 1. American Society of Microbiology, Washington DC.
33 Isenberg HD. 1992. Urease, pp. 2.6.8. Clinical Microbiology Procedures Handbook, Vol. 1. American Society of Microbiology, Washington, DC.
34 Ishibashi N, Yamazaki S. 2001. Probiotics and safety. Am. J. Clin. Nutr. 73: 465S-470S.   DOI
35 Chadwick RW, George SE, Clawton LD. 1992. Role of the gastrointestinal mucosa and microflora in the bioactivation of dietary and environmental mutagens or carcinogens. Drug Metabol. Rev. 24: 425-492.   DOI
36 Khoruts A, Dicksved J, Jansson JK, Sadowsky MJ. 2010. Changes in the composition of the human fecal microbiome after bacteriotherapy for recurrent Clostridium difficile-associated diarrhea. J. Clin. Gastroenter. 44: 354-360.
37 Khoruts A, Sadowsky MJ, Hamilton MJ. 2015. Development of fecal microbiota transplantation suitable for mainstream medicine. Clin. Gastroenterol. Hepatol. 13: 246-250.   DOI
38 Bruce-Keller AJ, Salbaum JM, Luo M, Blanchard E, Taylor CM, Welsh DA, Berthoud HR. 2015. Obese-type gut microbiota induce neurobehavioral changes in the absence of obesity. Biol. Psychiatry 77: 607-615.   DOI
39 Charteris WP, Kelly PM, Morelli L, Collins JK. 1998. Antibiotic susceptibility of potentially probiotic Lactobacillus species. J. Food Prot. 61: 1636-1643.   DOI
40 Cho IJ, Lee NK, Hahm YT. 2009. Characterization of Lactobacillus spp. isolated from the feces of breast-feeding piglets. J. Biosci. Bioeng. 108: 194-198.   DOI
41 Choi SY, Chang CE, Kim SC, So JS. 2003. Antimicrobial susceptibility and strain prevalence of Korean vaginal Lactobacillus spp. Anaerobe 9: 277-280.   DOI
42 D’Aimmo MR, Modesto M, Biavati B. 2007. Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products. Int. J. Food Microbiol. 115: 35-42.   DOI
43 European Committee on Antimicrobial Susceptibility Testing (EUCAST). 2015. Clinical breakpoints. Available at http://www.eucast.org/clinical_breakpoints.
44 Baccigalupi L, Di DA, Parlato M, Luongo D, Carbone V, Rossi M, et al. 2005. Small surface-associated factors mediate adhesion of a food-isolated strain of Lactobacillus fermentum to Caco-2 cells. Res. Microbiol. 156: 830-836.   DOI
45 Foligné B, Grangette C, Pot B. 2005. Probiotics in IBD: mucosal and systemic routes of administration may promote similar effects. Gut 54: 727-728.
46 Haza AI, Zabala A, Morales P. 2004. Protective effect and cytokine production of a Lactobacillus plantarum strain isolated from ewes’ milk cheese. Int. Dairy J. 14: 29-38.   DOI
47 An SJ, Pae HO, Oh GS, Choi BM, Jeong S, Jang SI, et al. 2002. Inhibition of TNF-α, IL-1β, and IL-6 productions and NF-κB activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated from Catalpa ovata G. Don (Bignoniaceae). Int. Immunopharmacol. 2: 1173-1181.   DOI