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http://dx.doi.org/10.4014/jmb.1505.05053

An Environmentally Friendly and Efficient Method for Extraction of PHB Biopolymer with Non-Halogenated Solvents  

Aramvash, Asieh (Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology)
Gholami-Banadkuki, Narges (Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology)
Moazzeni-Zavareh, Fatemeh (Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology)
Hajizadeh-Turchi, Samira (Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.11, 2015 , pp. 1936-1943 More about this Journal
Abstract
The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25℃ until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103℃ and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 106 compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.
Keywords
Butyl acetate; central composite design; Cupriavidus necator; design expert; ethyl acetate; response surface methodology;
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