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http://dx.doi.org/10.4014/jmb.1108.08087

Genetic and Phenotypic Diversity of Carbofuran-Degrading Bacteria Isolated from Agricultural Soils  

Shin, Dong-Hyeon (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Kim, Dong-Uk (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Seong, Chi-Nam (Department of Biology, Sunchon National University)
Song, Hong-Gyu (Division of Biological Sciences, Kangwon National University)
Ka, Jong-Ok (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.4, 2012 , pp. 448-456 More about this Journal
Abstract
Thirty-seven carbofuran-degrading bacteria were isolated from agricultural soils, and their genetic and phenotypic characteristics were investigated. The isolates were able to utilize carbofuran as a sole source of carbon and energy. Analysis of the 16S rRNA gene sequence indicated that the isolates were related to members of the genera Rhodococcus, Sphingomonas, and Sphingobium, including new types of carbofuran-degrading bacteria, Bosea and Microbacterium. Among the 37 isolates, 15 different chromosomal DNA patterns were obtained by polymerase chain reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences. Five of the 15 representative isolates were able to degrade carbofuran phenol, fenoxycarb, and carbaryl, in addition to carbofuran. Ten of the 15 representative isolates had 1 to 8 plasmids. Among the 10 plasmid-containing isolates, plasmid-cured strains were obtained from 5 strains. The cured strains could not degrade carbofuran and other pesticides anymore, suggesting that the carbofuran degradative genes were on the plasmid DNAs in these strains. When analyzed with PCR amplification and dot-blot hybridization using the primers targeting for the previously reported carbofuran hydrolase gene (mcd), all of the isolates did not show any positive signals, suggesting that their carbofuran hydrolase genes had no significant sequence homology with the mcd gene.
Keywords
Carbofuran-degrading bacteria; carbofuran hydrolase gene; biodegradation; diversity; carbamate insecticide;
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