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Immunomodulating Activities of Water-Soluble Exopolysaccharides Obtained from Submerged Culture of Lentinus lepideus  

Jung, Yu-Sun (Department of Biotechnology, Daegu University)
Yang, Byung-Keun (Research Center for Processing and Application of Agricultural Products, Daegu University)
Jeong, Yong-Tae (Department of Biotechnology, Daegu University)
Islam, Rezuanul (Department of Biotechnology, Daegu University)
Kim, Sang-Min (Department of Biotechnology, Daegu University)
Song, Chi-Hyun (Department of Biotechnology, Daegu University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.8, 2008 , pp. 1431-1438 More about this Journal
Abstract
Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged mycelial culture of Lentinus lepideus were studied and their effectiveness was compared with lipopolysaccharide (LPS). The influence of the LL-EX on macrophage cellular lysosomal enzyme activity was to stimulate up to 267%, 392%, and 464% at the level of 10, 50, and $100{\mu}g/ml$, respectively. When the LL-EX was further fractionated into LL-Fr.I and Fr.II by Sepharose CL-6B gel chromatography, the cellular lysosomal enzyme activity of LL-Fr.II (2.1-fold) was higher than Fr.I (1.2-fold). Moreover, both LL-Fr.I and Fr.II stimulated the cytokines IL-1$\beta$, TNF-$\alpha$, and IL-6 in macrophages. In mixed lymphocyte reaction, LL-Fr.I and Fr.II enhanced the splenocyte proliferation up to 1.2-fold and 1.4-fold ($50{\mu}g/ml$), respectively, stimulating only T lymphocytes. The fractions of LL-EX not show any direct toxicity against human gastric adenocarcinoma cell (AGS). The molecular masses of LL-Fr.I and Fr.II were estimated to be about 1,986 kDa and 21 kDa, respectively. The total sugar and protein contents of the two fractions were 84.97% and 69.88%, and 15.03% and 30.12%, respectively. The sugar and amino acid compositions of the LL-Fr.I and Fr.II were also analyzed in detail.
Keywords
Lentinus lepideus; immunomodulating; IL-1$\beta$; IL-6; TNF-$\alpha$; T lymphocyte;
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Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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