[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.0800.175

Isolation and Physiological Characterization of Bacillus clausii SKAL-16 Isolated from Wastewater

Lee, Sung-Hun (Department of Biological Engineering, Seokyeong University)
Park, Doo-Hyun (Department of Biological Engineering, Seokyeong University)

Publication Information

Journal of Microbiology and Biotechnology / v.18, no.12, 2008 , pp. 1908-1914 More about this Journal

Abstract

An alkaliphilic bacterium, Bacillus clausii SKAL-16, was isolated from soil that had been contaminated with vegetable oil. The optimal pH and general pH range for bacterial growth was 8, and 7 to 10, respectively. The bacterium could grow on tributyrin and glycerol, but could not grow on acetate and butyrate. The SKAL-16 strain excreted butyric acid during growth on tributyrin, and selectively ingested glycerol during growth on a mixture of butyric acid and glycerol. The SKAL-16 generated intracellular lipase, but did not produce esterase and extracellular lipase. The DNA fragment amplified with the chromosomal DNA of SKAL-16 and primers designed on the basis of the esterase-coding gene of Bacillus clausii KSM-KI6 was not identical with the esterase-coding gene contained in the GenBank database. Pyruvate dehydrogenase, isocitrate dehydrogenase, and malate dehydrogenase activities were detected in the cell-free extract (crude enzyme).

Keywords

Tributyrin; lipase; esterase; alkaliphile; Bacillus clausii;

Citations & Related Records

Times Cited By Web Of Science : 2 (Related Records In Web of Science)

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