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http://dx.doi.org/10.5713/ajas.17.0031

Antioxidant potential of buffalo and cow milk Cheddar cheeses to tackle human colon adenocarcinoma (Caco-2) cells  

Huma, Nuzhat (National Institute of Food Science and Technology, University of Agriculture)
Rafiq, Saima (Department of Food Science and Technology, The University of Poonch Rawalakot)
Sameen, Aysha (National Institute of Food Science and Technology, University of Agriculture)
Pasha, Imran (National Institute of Food Science and Technology, University of Agriculture)
Khan, Muhammad Issa (National Institute of Food Science and Technology, University of Agriculture)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.31, no.2, 2018 , pp. 287-292 More about this Journal
Abstract
Objective: The aim of present study was to assess the anti-oxidant potential of water-soluble peptides (WSPs) extract derived from buffalo and cow milk Cheddar cheeses at different stages of ripening. Methods: The antioxidant potential of WSPs extract was assessed through 2,2'-azinobis-3-ethylbenzothiazoline-6sulfonic acid (ABTS)-radical scavenging activity. In addition, impact of WSPs extract on cell viability and production of reactive oxygen species (ROS) in human colon adenocarcinoma Caco-2 (tert-butylhydroperoxide-induced) cell lines was also evaluated. Results: The ABTS-radical scavenging activity increased progressively with ripening period and dose-dependently in both cheeses. However, peptide extract from buffalo milk Cheddar cheese demonstrated relatively higher activity due to higher contents of water-soluble nitrogen. Intracellular ROS production in Caco-2 cells decreased significantly (p<0.05) till 150th day of cheese ripening and remained constant thereafter. Additionally, dose-dependent response of WSPs extract on antioxidant activity was noticed in the Caco-2 cell line. Conclusion: On the basis of current in vitro study, the Cheddar cheese WSPs extract can protect intestinal epithelium against oxidative stress due to their antioxidant activity.
Keywords
Cheddar Cheese; Bioactive Peptides; Caco-2 Cell Line; Antioxidant;
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