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http://dx.doi.org/10.5713/ajas.2012.12567

Effect of Dipeptides on In vitro Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes  

Tareq, K.M.A. (School of Biotechnology, Yeungnam University)
Akter, Quzi Sharmin (Department of Animal Breeding and Genetics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University)
Tsujii, Hirotada (Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University)
Khandoker, M.A.M. Yahia (Department of Animal Breeding and Genetics, Bangladesh Agricultural University)
Choi, Inho (School of Biotechnology, Yeungnam University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.26, no.4, 2013 , pp. 501-508 More about this Journal
Abstract
The effects of amino acids and dipeptides on in vitro production of porcine embryos and accumulation of ammonia in culture medium during developmental stages were examined in this study. The maturation, fertilization and development of embryonic cultures were performed in modified Tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, modified Tyrode's albumin lactate pyruvate (mTALP) medium, and modified North Carolina State University (mNCSU)-23 medium, respectively. In addition, amino acids and dipeptides of different concentrations and combinations were used to treat the embryos. The addition of L-alanyl-L-glutamine (AlnGln)+L-glycyl-L-glutamine (GlyGln) significantly (p<0.05) improved oocyte maturation, fertilization and the incorporation and oxidation of 14C(U)-glucose when compared to the control group and other treatment groups. Additionally, 2-4 cell, 8-16 cell, morula and blastocyst development increased significantly (p<0.05) following treatment with AlnGln+GlyGln when compared to the control group and other treatment groups, while this treatment reduced the accumulation of ammonia. Taken together, these findings suggest that treatment with AlnGln+GlyGln may play an important role in increasing the rate of porcine oocyte maturation, fertilization and embryonic development by reducing the level of accumulated ammonia measured in the culture media.
Keywords
Ammonia Accumulation; Dipeptides; Incorporation and Oxidation; Porcine Embryos;
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