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http://dx.doi.org/10.5713/ajas.2010.90313

Molecular Cloning and mRNA Expression of the Porcine Insulin-responsive Glucose Transporter (GLUT4)  

Zuo, Jianjun (College of Animal Science, South China Agricultural University)
Dai, Fawen (College of Animal Science, South China Agricultural University)
Feng, Dingyuan (College of Animal Science, South China Agricultural University)
Cao, Qingyun (College of Animal Science, South China Agricultural University)
Ye, Hui (College of Animal Science, South China Agricultural University)
Dong, Zemin (College of Animal Science, South China Agricultural University)
Xia, Weiguang (College of Animal Science, South China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.23, no.5, 2010 , pp. 640-648 More about this Journal
Abstract
Insulin-responsive glucose transporter 4 (GLUT4) is a member of the glucose transporter family and mainly presents in skeletal muscle and adipose tissue. To clarify the molecular structure of porcine GLUT4, RACE was used to clone its cDNA. Several cDNA clones corresponding to different regions of GLUT4 were obtained by amplifying reverse-transcriptase products of total RNA extracted from Landrace porcine skeletal muscles. Nucleotide sequence analysis of the cDNA clones revealed that porcine GLUT4 cDNA was composed of 2,491 base pairs with a coding region of 509 amino acids. The deduced amino acid sequence was over 90% identical to human, rabbit and cattle GLUT4. The tissue distribution of GLUT4 was also examined by Real-time RT-PCR. The mRNA expression abundance of GLUT4 was heart>liver, skeletal muscle and brain>lung, kidney and intestine. The developmental expression of GLUT4 and insulin receptor (IR) was also examined by Real-time RT-PCR using total RNA extracted from longissimus dorsi (LM), semimembranosus (SM), and semitendinosus (SD) muscle of Landrace at the age of 1, 7, 30, 60 and 90 d. It was shown that there was significant difference in the mRNA expression level of GLUT4 in skeletal muscles of Landrace at different ages (p<0.05). The mRNA expression level of IR also showed significant difference at different ages (p<0.05). The developmental change in the mRNA expression abundance of GLUT4 was similar to that in IR, and both showed a higher level at birth and 30 d than at other ages. However, there was no significant tissue difference in the mRNA expression of GLUT4 or IR (p>0.05). These results showed that the nucleotide sequence of the cDNA clones was highly identical with human, rabbit and cattle GLUT4 and the developmental change of GLUT4 mRNA in skeletal muscles was similar to that of IR, suggesting that porcine GLUT4 might be an insulin-responsive glucose transporter. Moreover, the tissue distribution of GLUT4 mRNA showed that GLUT4 might be an important nutritional transporter in porcine skeletal muscles.
Keywords
GLUT4; IR; Skeletal muscle; Porcine;
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1 Gaster, M., A. Handberg, H. Beck-Nielsen and H. D. Schroder. 2000. Glucose transporter expression in human skeletal muscle fibers. Am. J. Physiol. Endocrinol. Metab. 279(3):E529-538   PUBMED
2 He, J., M. Thamotharan and S. U. Devaskar. 2003. Insulin-induced translocation of facilitative glucose transporters in fetal/neonatal rat skeletal muscle. Am. J. Physiol. Regul. Integr. Comp. Physiol. 284(4):R1138-1146   DOI   PUBMED   ScienceOn
3 Joost, H. G., G. I. Bell, J. D. Best, M. J. Birnbaum, M. J. Charron, Y. T. Chen, H. Doege, D. E. James, H. F. Lodish, K. H. Moley, J. F. Moley, M. Mueckler, S. Rogers, A. Schurmann, S. Seino and B. Thorens. 2002. Nomenclature of the GLUT/SLC2A family of sugar/polyol transport facilitators. Am. J. Physiol. Endocrinol. Metab. 282(4):E974-976   PUBMED
4 Kitamura, T., Y. Kitamura, J. Nakae, A. Giordano, S. Cinti, C. R. Kahn, A. Efstratiadis and D. Accili. 2004. Mosaic analysis of insulin receptor function. J. Clin. Invest. 113(2):209-219   PUBMED   ScienceOn
5 Knott, R. M., G. Grant, S. Bardocz, A. Pusztai, de Carvalho AFFU and J. E. Hesketh. 1992. Alterations in the level of insulin receptor and GLUT-4 mRNA in skeletal muscle from rats fed a kidney bean (Phaseolus vulgaris) diet. Int. J. Biochem. 24(6):897-902   DOI   ScienceOn
6 Le, K. A., D. Faeh, R. Stettler, C. Debard, E. Loizon, H. Vidal, C. Boesch, E. Ravussin and L. Tappy. 2008. Effects of four-week high-fructose diet on gene expression in skeletal muscle of healthy men. Diabetes Metab. 34(1):82-85   DOI   ScienceOn
7 Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25(4):402-408   DOI   ScienceOn
8 Manso Filho, H. C., K. H. McKeever, M. E. Gordon, H. E. C. Costa and M. Watford. 2007. Novel findings regarding Glut-4 expression in adipose tissue and muscle in horses - A preliminary report. The Veterinary Journal 174(3):565   DOI   ScienceOn
9 Watson, R. T. and J. E .Pessin. 2007. GLUT4 translocation: The last 200 nanometers. Cell. Signal. 19:2209-2217   DOI   ScienceOn
10 Tonack, S., N. Ramin, S. Garimella, R. Rao, P. B. Seshagiri, B. Fischer and A. Navarrete Santos. 2009. Expression of glucose transporter isoforms and the insulin receptor during hamster preimplantation embryo development. Ann. Anat. - Anatomischer Anzeiger. 191(5):485-495   DOI   ScienceOn
11 Diaz, M., E. Capilla and J. V. Planas. 2007. Physiological regulation of glucose transporter (GLUT4) protein content in brown trout (Salmo trutta) skeletal muscle. J. Exp. Biol. 210(13):2346-2351   DOI   ScienceOn
12 Hall, J. R., C. E. Short and W. R. Driedzic. 2006. Sequence of Atlantic cod (Gadus morhua) GLUT4, GLUT2 and GPDH: developmental stage expression, tissue expression and relationship to starvation-induced changes in blood glucose. J. Exp. Biol. 209:4490-4502   DOI   ScienceOn
13 Gray, S., B. S. Stonestreet, S. Thamotharan, G. B. Sadowska, M. Daood, J. Watchko and S. U. Devaskar. 2006. Skeletal muscle glucose transporter protein responses to antenatal glucocorticoids in the ovine fetus. J. Endocrinol. 189(2):219-229   DOI   ScienceOn
14 He, A. B., X. J. Liu, L. Z. Liu, Y. S. Chang and F. Fang. 2007. How many signals impinge on GLUT4 activation by insulin. Cell. Signal. 19(1):1-7   DOI   ScienceOn
15 Walker, P. S., T. Ramlal, V. Sarabia, U. M. Koivisto, P. J. Bilan, J. E. Pessin and A. Klip. 1990. Glucose transport activity in L6 muscle cells is regulated by the coordinate control of subcellular glucose transporter distribution, biosynthesis, and mRNA transcription. J. Biol. Chem. 265(3):1516-1523   PUBMED
16 Zhao, F. Q. and A. F. Keating. 2007. Functional properties and genomics of glucose transporters. Curr. Genomics 8(2):113-128   DOI   ScienceOn
17 Scheffler, T. L. and D. E. Gerrard. 2007. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci. 77(1):7-16   DOI   ScienceOn
18 Ferguson, D. M., B. L. Daly, G. E. Gardner and R. K. Tume. 2008. Effect of glycogen concentration and form on the response to electrical stimulation and rate of post-mortem glycolysis in ovine muscle. Meat Sci. 78(3):202-210   DOI   ScienceOn
19 Heled, Y., Y. Dror, D. S. Moran, T. Rosenzweig, S. R. Sampson, Y. Epstein and J. Meyerovitch. 2005. Physical exercise increases the expression of TNF[alpha] and GLUT 1 in muscle tissue of diabetes prone Psammomys obesus. Life Sci. 77(23):2977-2985   DOI   ScienceOn
20 Saltiel, A. R. and J. E. Pessin. 2002. Insulin signaling pathways in time and space. Trends Cell Biol. 12(2):65-71   DOI   ScienceOn
21 Humphrey, B. D., C. B. Stephensen, C. C. Calvert and K. C. Klasing. 2004. Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus). Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. 138(4):515-525   DOI   ScienceOn
22 Hou, J. C. and J. E. Pessin. 2007. Ins (endocytosis) and outs (exocytosis) of GLUT4 trafficking. Cur. Opin. Cell Biol. 19(4):466-473   DOI   ScienceOn