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http://dx.doi.org/10.5713/ajas.2009.80690

Effects of Glucagon-like Peptide-2 on Morphology, Proliferation and Enzyme Activity of Intestinal Enterocyte Cells of Weaned Piglets In vitro  

Jia, Gang (Animal Nutrition Institute of Sichuan Agricultural University)
Jiang, RongChuan (Animal Nutrition Institute of Sichuan Agricultural University)
Wang, KangNing (Animal Nutrition Institute of Sichuan Agricultural University,Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.8, 2009 , pp. 1160-1166 More about this Journal
Abstract
This study was conducted according to the single-factor design principle to investigate in vitro the effects of different glucagon-like peptide-2 (GLP-2) concentrations (0, $1{\times}10^{-11}$, $1{\times}10^{-10}$, $1{\times}10^{-9}$, $1{\times}10^{-8}$ and $1{\times}10^{-7}$ mol/L) on the morphology, proliferation and enzyme activity of intestinal enterocyte cells of 28-d-old weaned piglets. These cells were primary cultured in 4 pieces of 24-well cell culture plate. After having been grown for 48 h in culture media with hGLP-2, the ileal enterocyte cells of 28-d-old weaned piglets exhibited the typical characteristics of simple columnar epithelium. Compared with the control groups, the quantities of treated cells significantly increased (p<0.05) and their corresponding absorption values in 540 nm (MTT OD) also significantly increased (p<0.01). Likewise, lactic acid concentration, total protein content and protein retention significantly increased (p<0.05). $Na^{+}$, $K^{+}$-ATP enzyme activity was more active (p<0.05), although the activity of alkaline phosphatase, lactic acid dehydrogenase and creatine phosphokinase in culture media significantly decreased (p<0.01). To summarize, the results indicated that GLP-2 in vitro is capable of promoting the proliferation of intestinal enterocyte cells of 28-d weaned piglets, restraining their apoptosis and maintaining the integrity of their morphology.
Keywords
Glucagon-like Peptide-2, Weaned Piglets; Intestinal Enterocyte Cell; Cell Proliferation; Enzyme Activity;
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