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http://dx.doi.org/10.5713/ajas.2003.1177

Effects of Cod Liver Oil and Chromium Picolinate Supplements on the Serum Traits, Egg Yolk Fatty Acids and Cholesterol Content in Laying Hens  

Lien, Tu-Fa (Department of Animal Science, National Chiayi University)
Wu, Chaen-Ping (Department of Animal Science, National Chiayi University)
Lu, Jin-Jenn (Department of Animal Science, National Chiayi University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.16, no.8, 2003 , pp. 1177-1181 More about this Journal
Abstract
This study investigated the effects of cod liver oil and chromium picolinate on the serum traits and egg yolk fatty acids and cholesterol content in laying hens. One hundred 45-week old single comb white Leghorn laying hens were assigned randomly to four groups. These groups were: (1) control (soybean oil), (2) 1,000 ppb (${\mu}g/kg$) chromium (organic form chromium picolinate) (Crpic), (3) 3% cod liver oil (CLO), and (4) 1,000 ppb chromium with 3% cod liver oil (CLO+Crpic). The experiment was conducted for 40 days. Results indicated that serum triacylglycerol (TG) and cholesterol contents in the CLO group and the serum glucose content in the Crpic group were significantly lower than those in the control group (p<0.05-0.01). The yolk cholesterol content in the CLO and Crpic groups were also lower than the control group (p<0.01). The lipoprotein profile displayed that in the Crpic group, high-density lipoprotein (HDL) and HDL-cholesterol (HDL-C) were significantly higher (P<0.05) than the control group. Meanwhile, low-density lipoprotein+very low-density lipoprotein (LDL+VLDL) and LDL-C+VLDL-C were significantly lower (p<0.05) than the control group. Notably, of all four groups, the CLO group displayed a more profound effect on serum traits and lipoprotein (p<0.05-0.001). Furthermore, the fatty acid composition of the egg yolks presented that C18:2 in the CLO and Crpic groups was significantly lower (p<0.05-0.001) compare to the control. However, only in the CLO group, C18:3, C20:5 and C22:6 were significantly higher (p<0.001) than the control. Only serum glucose and LDL+VLDL showed the CLO${\times}$Crpic interaction (p<0.05), most parameters did not. Therefore, supplemented chromium picolinate or cod liver oil in the diet of laying hens had beneficial effects. However, when these two factors were combined, there was no interaction with most parameters.
Keywords
Cod Liver Oil; Chromium Picolinate; Serum Traits; Egg Yolk Cholesterol; Laying Hens;
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1 Sanders, T. A. B., M. Vickers and A. P. Haines. 1981. Effect of blood lipids and haemostasis of a supplement of cod liver oil, rich in eicosapentaenoic and decosahexaenoic acids, in healthy young men. Clin. Sci. 61:317-324.   DOI
2 Xi G., Z. Xu, S. Wu and S. Chen. 2001. Effect of chromium picolinate on growth performance, carcass characteristics, serum metabolites and metabolism of lipid in pigs. Asian-Aust. J. Anim. Sci. 14:258-262.
3 Anderson, R. A., M. M. Polansky, N. A. Bryden, S. J. Bhathena and J. J. Canary. 1987. Effects of supplemental chromium on patients with symptoms of reactive hypoglycemia. Metab. 38: 351-355.
4 Beyer, R. and L. S. Jensen. 1989. Overestimation of the cholesterol content of eggs. J. Agr. and Food Chem. 37:917-920.   DOI
5 Kim, D. N., J. Schmee, C. S. Lee, A. Eastman, J. S. Ross and W. A. Thomas. 1991. Composition of effects of fish oil and corn oil supplements on hyperlipidemic diet induced atherogenesis in swine. Atherosclerosis 89:191-201.   DOI
6 Brindley, D. N. and A. M. Salter. 1991. Hormonal regulation of the hepatic low density lipoprotein: relationship with the secretion of very low density lipoprotein. J. Lipid Res. 30:349-360.   DOI   ScienceOn
7 Cobb, S. A. and J. L. Sanders. 1978. Enzymic determination of cholesterol in serum lipoproteins separated by electrophoresis. Clin. Chem. 24:1116.
8 Evock-Clover, C. M., M. M. Polansky, R. A. Anderson and N. C. Steel. 1993. Dietary chromium supplementation with or without somatotropin treatment alters serum hormones and metabolites in growing pigs without affecting growth performance. J. Nutr. 123:1504-1512.
9 Lien, T. F., Y. M. Horng and K. H. Yang. 1999. performance, serum characteristics, carcass traits and lipid metabolism of broilers as affected by supplement of chromium picolinate. Br. Poult. Sci. 40:357-363.   DOI   ScienceOn
10 Lien, T. F., C. P. Wu, B. J. Wang, M. S. Shiao, T. Y. Shiao, B. H. Lin and C. Y. Hu. 2001. Effect of supplemental levels of chromium picolinate on the growth performance, serum traits, carcass characteristics and lipid metabolism of growingfinishing pigs. Anim. Sci. 72:289-296.
11 Lindemann, M. D., C. M. Wood, A. F. Harper, E. T. Kornegay and R. A. Anderson. 1995. Dietary chromium picolinate additions improve gain:feed and carcass characteristics in growing finishing pigs and increase litter size in reproducing sows. J. Anim. Sci. 73:457-465.   DOI
12 Hargis, P. S., M. E. Van Elswyk and B. M. Hargis. 1991. Dietary modification of yolk lipid with menhaden oil. Poult. Sci. 70: 874-883.   DOI   ScienceOn
13 Kim, Y. H., I. K. Han, Y. J. Choi, I. S. Shin, B. J. Chae and T. H. Kang. 1996. Effects of dietary levels of chromium picolinate on growth performance, carcass quality and serum traits in broiler chicks. Asian-Aust. J. Anim. Sci. 9:341-347.
14 Wang, M. M., E. A. Fox, B. J. Stoecker, C. E. Menendez and S. B. Chan. 1989. Serum cholesterol of adults supplemented with brewer's yeast or chromium chloride. Nutr. Res. 9:989-998.   DOI   ScienceOn
15 Lien, T. F., S. Y. Chen, C. P. Wu, C. L. Chen and C. Y. Hu. 1996. Effects of chromium picolinate and chromium chloride on growth performance and serum traits of growing-finishing swine. Proceeding of Western Section, American Society of Animal. Science 47:150-153.
16 Page, T. G., L. L. Southern, T. L. Ward and Jr. D. L. Thompson. 1993. Effect of chromium picolinate on growth and serum and carcass traits of growing-finishing pigs. J. Anim. Sci. 71:656-662.   DOI
17 Press, R. I., J. Geller. and G. W. Evans. 1990. The effect of chromium picolinate on serum cholesterol and apolipoprotein fractions in human subjects. The Western J. Medicine 152:41-45.
18 Sanders, T. A. B. and F. Roshani. 1983. The influence of different types of $\omega$-3 polyunsaturated fatty acids on blood lipids and platelet function in healthy voluntees. Clin. Sci. 64:91-99.   DOI
19 Weintraub, M. S., R. Zechner, A. Brown, S. Eisenberg and L. Breslow. 1988. Dietary polyunsaturated fats of the $\omega$-6 and $\omega$-3 series reduce postprandial lipoprotein levels. J. Clin. Invest. 82:1884-1893.   DOI   ScienceOn
20 Evans, G. W. 1989. The effect of chromium picolinate on insulin controlled parameters in humans. Intern. J. Biophys. and Med. Res. 11:163-180.
21 Evans, G. W. and T. D. Bowman. 1992. Chromium picolinate increases membrane fluidity and rate of insulin internalization. J. Inorg. Biochem. 46:243-250.   DOI   ScienceOn
22 Anderson, R. A., N. K. Bryden and M. M. Polansky. 1985. Serum chromium of human subjects: effects of chromium supplementation and glucose metablism. Am. J. Clin. Nutr. 41:571-577.
23 Anderson, R. A., 1986. Chromium metabolism and its role in disease processes in man. Clin. Physio. Biochem. 4:31-41.
24 Van-Elswky, M. E., B. M. Hargis, J. D. Williams and P. S. Hargis. 1994. Dietary menhaden oil contributes to hepatic lipidosis in laying hens. Poult. Sci. 73:653-662.   DOI   ScienceOn
25 Howard, B. V., N. Schneiderman, B. Falkner, S. M. Haffner and A. Laws. 1993. Insulin, health behaviors, and lipid metabolism. Metab. 42 (suppl. 1):25-35.   DOI   ScienceOn
26 Jiang, Z. and J. S. Sim. 1992. Effects of dietary n-3 fatty acid enriched chicken eggs on plasma and tissue cholesterol and fatty acid composition of rats. Lipid 27:279-284.   DOI   ScienceOn
27 Lien, T. F., S. Y. Chen, S. P. Shiau, D. P. Froman and C. Y. Hu. 1997. Chromium picolinate reduces laying hen serum and egg yolk cholesterol. The Profes. Anim. Scient. 12:77-80.
28 Statistical Analysis System Institute. 1990. SAS/STAT User's guide, Version 6.06, 4th ed. Cary, NC.
29 Mirsky, N. 1993. Glucose tolerance factor reduces blood glucose and fatty acids levels in diabetic rats. J. Inorg. Biochem. 49: 123-128.   DOI   PUBMED   ScienceOn
30 Simopoulos, A. P. and N. Jr. Salem. 1992. Egg yolk as a source of long-chain polyunsaturated fatty acid in infant feeding. Am. J. Clin. Nutr. 55:411-414.   DOI
31 Lefavi, R. G., G. D. Wilson, R. E. Keith, R. A. Anderson, D. L. Blessing, C. G. Hames and J. L. McMillan. 1993. Lipidlowering effect of a dietary chromium (III) nicotinic acid complex in male athletes. Nutr. Res. 13:239-249.   DOI   ScienceOn
32 Oh, S. Y., C. H. H. Lin, J. Ryue and D. Brown. 1994. Eggs enriched with omega-3 fatty acids as a wholesome food. J. Appl. Nutr. 46: 15-25.
33 Morris, B. W., T. A. Gray and S. Macneil. 1993. Glucosedependent uptake of chromium in human and rat insulinsensitive tissues. Clin. Sci. 84:477-482.   DOI
34 Okada, S., H. Tsukada and H. Ohba. 1984. Enhancement of nucleo RNA synthesis by chromium (III) in regenerating rat liver. J. Inorg. Biochem. 21:113-119.   DOI   ScienceOn
35 Houstmuller, A. J. 1969. Agarose-gel electrophoresis of lipoproteins: A clinical screening test. Edited by Koninkliike Van Gorcum and Comp. The Netherlands, p. 5.
36 Vilchez, C., S. P. Touchburn, E. R. Chavez and C. W. Chan. 1991. Effect of feeding palmitic, oleic, and linoleic acids to Japanese quail hens (Coturnix coturnix japonica). 1. Reproductive performance and tissue fatty acids. Poult. Sci. 70:2484-2493.   DOI   ScienceOn
37 Sanders, T. A. B., D. R. Sullivan, J. Reeve and G. R. Thompson. 1985. Triglyceride-lowering effect of marine polyunsaturates in patients with hypertriglyceridemia. Arteriosclerosis 5:459-465.   DOI
38 McCarty, M. F. 1991. The case for supplemental chromium and a survey of clinical studies with chromium picolinate. J. Apply Nutr. 43:58-66.
39 Amoikon, E. K., J. M. Fernandez, L. L. Southern, D. L. Thompson, Jr. T. L. Ward and B. M. Olcott. 1995. Effect of chromium tripicolinate on growth glucose tolerance, insulin sensitivity, plasma metabolites, and growth hormone in pigs. J. Anim. Sci. 73:1123-1130.
40 Lien, T. F., C. P. Wu, P. H. Lin, B. J. Wang, J. J. Lu and T. Y. Shiao. 1998. Effect of different protein and limiting amino acid levels coupled with a supplement of chromium picolinate on lipid metabolism and carcass characteristics of pigs. Anim. Sci. 67: 601-607.   DOI
41 Cupo, M. A. and W. E. Donaldson. 1987. Chromium and vanadium effect on glucose metabolism and lipids synthesis in the chick. Poult. Sci. 66:120-126.   DOI   ScienceOn
42 Rosebrough, R. W. and N. C. Steele. 1981. Effects of supplemental dietary chromium or nicotinic acid on carbohydrate metabolism during basal, starvation, and refeeding periods in poults. Poult. Sci. 60:407-417.   DOI   ScienceOn
43 Anderson, R. A., M. P. Polansky, N. A. Bryden and J. J. Canary. 1991. Supplemental chromium effects on glucose, insulin, glucagon and urinary chromium losses in subjects consuming controlled low-chromium diets. Am. J. Clin. Nutr. 54:909-916.