Browse > Article
http://dx.doi.org/10.1080/19768354.2011.604946

Expression of pro-opiomelanocortin and agouti-related protein in the hypothalamus of caffeine-administered rats  

Jeong, Joo-Yeon (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Ku, Bo-Mi (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Lee, Yeon-Kyung (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Ryu, Jin-Hyun (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Choi, Jung-Il (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Kim, Joon-Soo (Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University, School of Medicine)
Cho, Yong-Woon (Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University, School of Medicine)
Roh, Gu-Seob (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Kim, Hyun-Joon (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Cho, Gyeong-Jae (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Choi, Wan-Sung (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Kang, Sang-Soo (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University)
Publication Information
Animal cells and systems / v.15, no.3, 2011 , pp. 203-210 More about this Journal
Abstract
In the present study, we examined the effects of caffeine on food intake and body weight, and pro-opiomelanocortin (POMC) and agouti-related protein (AgRP) expression in the hypothalamus. Rats were administered intraperitoneally with 100 mg/kg caffeine (a high, non-toxic dose) or saline during the light phase. Intraperitoneal administration of caffeine induced a significant reduction in food intake and body weight 12 hr after treatment. In addition, POMC expression was significantly increased and AgRP expression was decreased in the arcuate nucleus (Arc) after caffeine treatment. These results demonstrate that administration of caffeine up-regulates POMC expression and down-regulates AgRP expression in the Arc, suggesting that the activation of the hypothalamic POMC neurons and inhibition of the AgRP neurons might play a role in the regulation of food intake and body weight by caffeine.
Keywords
body weight; food intake; POMC; AgRP; caffeine;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Adan RA, Oosterom J, Toonen RF, Kraan MV, Burbach JP, Gispen WH. 1997. Molecular pharmacology of neural melanocortin receptors. Receptors Channels. 5(3-4):215- 223.
2 Astrup A, Breum L, Toubro S, Hein P, Quaade F. 1992. The effect and safety of an ephedrine/caffeine compound compared to ephedrine, caffeine and placebo in obese subjects on an energy restricted diet. A double blind trial. Int J Obes Relat Metab Disord. 16(4):269-277.
3 Belgardt BF, Okamura T, Bruning JC. 2009. Hormone and glucose signaling in POMC and AgRP neurons. J Physiol. 587(Pt 22):5305-5314.   DOI   ScienceOn
4 Bouret SG, Draper SJ, Simerly RB. 2004. Formation of projection pathways from the arcuate nucleus of the hypothalamus to hypothalamic regions implicated in the neural control of feeding behavior in mice. J Neurosci. 24(11):2797-2805.   DOI   ScienceOn
5 Broberger C, Hokfelt T. 2001. Hypothalamic and vagal neuropeptide circuitries regulating food intake. Physiol Behav. 74(4-5):669-682.   DOI
6 Chen AS, Marsh DJ, Trumbauer ME, Frazier EG, Guan XM, Yu H, Rosenblum CI, Vongs A, Feng Y, Cao L, Metzger JM, Strack AM, Camacho RE, Mellin TN, Nunes CN, Min W, Fisher J, Gopal-Truter S, MacIntyre DE, Chen HY, Van der Ploeg LH. 2000. Inactivation of the mouse melanocortin-3 receptor results in increased fat mass and reduced lean body mass. Nat Genet. 26(1):97-102.   DOI   ScienceOn
7 Cone RD. 1999. The central melanocortin system and energy homeostasis. Trends Endocrinol Metab. 10(6):211-216.   DOI   ScienceOn
8 Cone RD. 2005. Anatomy and regulation of the central melanocortin system. Nat Neurosci. 8(5):571-578.   DOI   ScienceOn
9 Simpson KA, Martin NM, Bloom SR. 2009. Hypothalamic regulation of food intake and clinical therapeutic applications. Arq Bras Endocrinol Metabol. 53(2):120-128.   DOI
10 SchwartzMW, Woods SC, Porte Jr D, Seeley RJ, Baskin DG. 2000. Central nervous system control of food intake. Nature. 404(6778):661-671.   DOI
11 Singewald N, Salchner P, Sharp T. 2003. Induction of c-Fos expression in specific areas of the fear circuitry in rat forebrain by anxiogenic drugs. Biol Psychiatry. 53(4):275-283.   DOI   ScienceOn
12 Svenningsson P, Nomikos GG, Fredholm BB. 1999. The stimulatory action and the development of tolerance to caffeine is associated with alterations in gene expression in specific brain regions. J Neurosci. 19(10):4011-4022.
13 Westerterp-Plantenga MS. 2010. Green tea catechins, caffeine and body-weight regulation. Physiol Behav. 100(1):42-46.   DOI   ScienceOn
14 Wirth MM, Giraudo SQ. 2000. Agouti-related protein in the hypothalamic paraventricular nucleus: effect on feeding. Peptides. 21:1369-1375.   DOI   ScienceOn
15 Mounien L, Bizet P, Boutelet I, Vaudry H, Jegou S. 2005. Expression of melanocortin MC3 and MC4 receptor mRNAs by neuropeptide Y neurons in the rat arcuate nucleus. Neuroendocrinology. 82(3-4):164-170.   DOI   ScienceOn
16 Lee CH, Choi JH, Hwang IK, Yoo KY, Li H, Park OK, Yan B, Shin HC, Won MH. 2009. Immunohistochemical changes in orexigenic and anorexigenic neuropeptides in the rat hypothalamus after capsaicin administration. J Vet Med Sci. 71(10):1337-1342.   DOI   ScienceOn
17 Lenard NR, Berthoud HR. 2008. Central and peripheral regulation of food intake and physical activity: pathways and genes. Obesity (Silver Spring). Suppl 3:S11-S22.
18 Mendez M, Morales-Mulia M, Perez-Luna JM. 2008. Ethanol-induced changes in proenkephalin mRNA expression in the rat nigrostriatal pathway. J Mol Neurosci. 34(3):225-234.   DOI   ScienceOn
19 Mountjoy KG, Mortrud MT, Low MJ, Simerly RB, Cone RD. 1994. Localization of the melanocortin-4 receptor (MC4-R) in neuroendocrine and autonomic control circuits in the brain. Mol Endocrinol. 8(10):1298-1308.   DOI   ScienceOn
20 Murphy JA, Deurveilher S, Semba K. 2003. Stimulant doses of caffeine induce c-Fos activation in orexin/hypocretincontaining neurons in rat. Neuroscience. 121:269-275.   DOI   ScienceOn
21 Ofluoglu E, Pasaoglu H, Pasaoglu A. 2009. The effects of caffeine on L-arginine metabolism in the brain of rats. Neurochem Res. 34(3):395-399.   DOI   ScienceOn
22 Rossi M, Kim MS, Morgan DG, Small CJ, Edwards CM, Sunter D, Abusnana S, Goldstone AP, Russell SH, Stanley SA, Smith DM, Yagaloff K, Ghatei MA, Bloom SR. 1998. A C-terminal fragment of Agouti-related protein increases feeding and antagonizes the effect of alpha-melanocyte stimulating hormone in vivo. Endocrinology. 139(10):4428-4431.   DOI   ScienceOn
23 Ollmann MM, Wilson BD, Yang YK, Kerns JA, Chen Y, Gantz I, Barsh GS. 1997. Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein. Science. 278:135-138.   DOI   ScienceOn
24 Pettenuzzo LF, Noschang C, von Pozzer Toigo E, Fachin A, Vendite D, Dalmaz C. 2008. Effects of chronic administration of caffeine and stress on feeding behavior of rats. Physiol Behav. 95(3):295-301.   DOI   ScienceOn
25 Pritchard LE, Turnbull AV, White A. 2002. Pro-opiomelanocortin processing in the hypothalamus: impact on melanocortin signalling and obesity. J Endocrinol. 172(3):411-421.   DOI
26 Diepvens K, Westerterp KR, Westerterp-Plantenga MS. 2007. Obesity and thermogenesis related to the consumption of caffeine, ephedrine, capsaicin, and green tea. Am J Physiol Regul Integr Comp Physiol. 292(1):R77-R85.   DOI
27 Kovacs EM, Lejeune MP, Nijs I, Westerterp-Plantenga MS. 2004. Effets of green tea on weight maintenance after body-weigh loss. Br J Nutr. 91(3):431-437.   DOI   ScienceOn
28 Lage R, Dieguez C, Lopez M. 2006. Caffeine treatment regulates neuropeptide S system expression in the rat brain. Neurosci Lett. 410(1):47-51.   DOI   ScienceOn
29 Cummings DE, Schwartz MW. 2000. Melanocortins and body weight: a tale of two receptors. Nat Genet. 26:8-9.   DOI   ScienceOn
30 Deurveilher S, Lo H, Murphy JA, Burns J, Semba K. 2006. Differential c-Fos immunoreactivity in arousal-promoting cell groups following systemic administration of caffeine in rats. J Comp Neurol. 498(5):667-689.   DOI   ScienceOn
31 Dulloo AG. 1993. Ephedrine, xanthines and prostaglandininhibitors: actions and interactions in the stimulation of thermogenesis. Int J Obes Relat Metab Disord. 17 Suppl 1:S35-S40.
32 Greenberg JA, Boozer CN, Geliebter A. 2006. Coffee, diabetes, and weight control. Am J Clin Nutr. 84:682-693.   DOI
33 Haskell-Luevano C, Monck EK. 2001. Agouti-related protein functions as an inverse agonist at a constitutively active brain melanocortin-4 receptor. Regul Pept. 99(1):1-7.   DOI   ScienceOn
34 Hillebrand JJ, de Wied D, Adan RA. 2002. Neuropeptides, food intake and body weight regulation: a hypothalamic focus. Peptides. 23(12):2283-2306.   DOI   ScienceOn
35 Huszar D, Lynch CA, Fairchild-Huntress V, Dunmore JH, Fang Q, Berkemeier LR, Gu W, Kesterson RA, Boston BA, Cone RD, Smith FJ, Campfield LA, Burn P, Lee F. 1997. Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell. 88(1):131-141.   DOI   ScienceOn
36 Jegou S, Delbende C, Tranchand-Bunel D, Leroux P, Vaudry H. 1987. alpha-Melanocyte-stimulating hormone (alpha- MSH) release from perifused rat hypothalamic slices. Brain Res. 413(2):259-266.   DOI   ScienceOn
37 Kishi T, Aschkenasi CJ, Lee CE, Mountjoy KG, Saper CB, Elmquist JK. 2003. Expression of melanocortin 4 receptor mRNA in the central nervous system of the rat. J Comp Neurol. 457(3):213-235.   DOI   ScienceOn