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http://dx.doi.org/10.14348/molcells.2017.0001

DDX53 Regulates Cancer Stem Cell-Like Properties by Binding to SOX-2  

Kim, Youngmi (Department of Biochemistry, Kangwon National University)
Yeon, Minjeong (Department of Biochemistry, Kangwon National University)
Jeoung, Dooil (Department of Biochemistry, Kangwon National University)
Publication Information
Molecules and Cells / v.40, no.5, 2017 , pp. 322-330 More about this Journal
Abstract
This study investigated the role of cancer/testis antigen DDX53 in regulating cancer stem cell-like properties. DDX53 shows co-expression with CD133, a marker for cancer stem cells. DDX53 directly regulates the SOX-2 expression in anti-cancer drug-resistant $Malme3M^R$ cells. DDX53 and miR-200b were found to be involved in the regulation of tumor spheroid forming potential of Malme3M and $Malme3M^R$ cells. Furthermore, the self-renewal activity and the tumorigenic potential of $Malme3M^R$-CD133 (+) cells were also regulated by DDX53. A miR-200b inhibitor induced the direct regulation of SOX-2 by DDX53 We therefore, conclude that DDX53 may serve as an immunotherapeutic target for regulating cancer stem-like properties of melanomas.
Keywords
anti-cancer drug-resistance; cancer stem cell-like properties; DDX53; SOX-2;
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1 Ahmad, A., Maitah, M.Y., Ginnebaugh, K.R., Li, Y., Bao, B., Gadgeel, S.M., and Sarkar, F.H. (2013). Inhibition of Hedgehog signaling sensitizes NSCLC cells to standard therapies through modulation of EMT-regulating miRNAs. J. Hematol. Oncol. 6, 77.   DOI
2 Chien, C.S., Wang, M.L., Chu, P.Y., Chang, Y.L., Liu, W.H., Yu, C.C., Lan, Y.T., Huang, P.I., Lee, Y.Y., Chen, Y.W., et al. (2015). Lin28B/Let-7 regulates expression of Oct4 and Sox2 and reprograms oral squamous cell carcinoma cells to a stem-like state. Cancer Res. 75, 2553-2565.   DOI
3 Cho, B., Lim, Y., Lee, D.Y., Park, S.Y., Lee, H., Kim, W.H., Yang, H., Bang, Y.J., and Jeoung, D.I. (2002). Identification and characterization of a novel cancer/testis antigen gene CAGE. Biochem. Biophys. Res. Commun. 292, 715-726.   DOI
4 Cho, B., Lee, H., Jeong, S., Bang, Y.J., Lee, H.J., Hwang, K.S., Kim, H.Y., Lee, Y.S., Kang, G.H., and Jeoung, D.I. (2003). Promoter hypomethylation of a novel cancer/testis antigen gene CAGE is correlated with its aberrant expression and is seen in premalignant stage of gastric carcinoma. Biochem. Biophys. Res. Commun. 307, 52-63.   DOI
5 Cho, Y.H., Han, K.M., Kim, D., Lee, J., Lee, S.H., Choi, K.W., Kim, J., and Han, Y.M. (2014). Autophagy regulates homeostasis of pluripotency-associated proteins in hESCs. Stem Cells 32, 424-435.   DOI
6 Chung, H.J., Choi, Y.E., Kim, E.S., Han, Y.H., Park, M.J., and Bae, I.H. (2015). miR-29b attenuates tumorigenicity and stemness maintenance in human glioblastoma multiforme by directly targeting BCL2L2. Oncotarget 6, 18429-18444.   DOI
7 Cox, J.L., Mallanna, S.K., Luo, X., and Rizzino, A. (2010). Sox2 uses multiple domains to associate with proteins present in Sox2-protein complexes. PLoS One 5, e15486.   DOI
8 Du, J., Liu, S., He, J., Liu, X., Qu, Y., Yan, W., Fan, J., Li, R., Xi, H., Fu, W., et al. (2015). MicroRNA-451 regulates stemness of side population cells via PI3K/Akt/mTOR signaling pathway in multiple myeloma. Oncotarget 6, 14993-15007.   DOI
9 Iwata, T., Fujita, T., Hirao, N., Matsuzaki, Y., Okada, T., Mochimaru, H., Susumu, N., Matsumoto, E., Sugano, K., Yamashita, N., Nozawa, S., and Kawakami, Y. (2005). Frequent immune responses to a cancer/testis antigen, CAGE, in patients with microsatellite instabilitypositive endometrial cancer. Clin. Cancer Res. 11, 3949-3957.   DOI
10 Jiang, J., Li, Z., Yu, C., Chen, M., Tian, S., and Sun, C. (2015). MiR-1181 inhibits stem cell-like phenotypes and suppresses SOX2 and STAT3 in human pancreatic cancer. Cancer Lett. 356, 962-970.   DOI
11 Kim, Y., Kim, H., Park, D., and Jeoung, D. (2015). miR-335 Targets SIAH2 and confers sensitivity to anti-cancer drugs by increasing the expression of HDAC3. Mol. Cells 38, 562-572.   DOI
12 Kim, Y., Park, H., Park, D., Lee, Y.S., Choe, J., Hahn, J.H., Lee, H., Kim, Y.M., and Jeoung, D. (2010). Cancer/testis antigen CAGE exerts negative regulation on p53 expression through HDAC2 and confers resistance to anti-cancer drugs. J. Biol. Chem. 285, 25957-25968.   DOI
13 Kim, Y., Park, D., Kim, H., Choi, M., Lee, H., Lee, Y.S., Choe, J., Kim, Y.M., and Jeoung, D. (2013). miR-200b and cancer/testis antigen CAGE form a feedback loop to regulate the invasion and tumorigenic and angiogenic responses of a cancer cell line to microtubuletargeting drugs. J. Biol. Chem. 288, 36502-36518.   DOI
14 Kim, Y., Kim, H., Park, H., Park, D., Lee, H., Lee, Y.S., Choe, J., Kim, Y.M., and Jeoung, D. (2014). miR-326-histone deacetylase-3 feedback loop regulates the invasion and tumorigenic and angiogenic response to anti-cancer drugs. J. Biol. Chem. 289, 28019-28039.   DOI
15 Kim, Y., Kim, H., Park, D., Han, M., Lee, H., Lee, Y.S., Choe, J., Kim, Y.M., and Jeoung, D. (2016). miR-217 and CAGE form feedback loop and regulates the response to anti-cancer drugs through EGFR and HER2. Oncotarget 7, 10297-10321.   DOI
16 Liggins, A.P., Lim, S.H., Soilleux, E.J., Pulford, K., and Banham, A.H. (2010). A panel of cancer-testis genes exhibiting broad-spectrum expression in haematological malignancies. Cancer Immun. 10, 8.
17 Lopez-Bertoni, H., Lal, B., Li, A., Caplan, M., Guerrero-Cazares, H., Eberhart, C.G., Quinones-Hinojosa, A., Glas, M., Scheffler, B., Laterra, J., and Li, Y. (2015). DNMT-dependent suppression of microRNA regulates the induction of GBM tumor-propagating phenotype by Oct4 and Sox2. Oncogene 34, 3994-4004.   DOI
18 Murakami, A., Takahashi, F., Nurwidya, F., Kobayashi, I., Minakata, K., Hashimoto, M., Nara, T., Kato, M., Tajima, K., Shimada, N., et al. (2014). Hypoxia increases gefitinib-resistant lung cancer stem cells through the activation of insulin-like growth factor 1 receptor. PLoS One 9, e86459.   DOI
19 Lu, Y.X., Yuan, L., Xue, X.L., Zhou, M., Liu, Y., Zhang, C., Li, J.P., Zheng, L., Hong, M., and Li, X.N. (2014). Regulation of colorectal carcinoma stemness, growth, and metastasis by an miR-200c-Sox2-negative feedback loop mechanism. Clin. Cancer Res. 20, 2631-2642.   DOI
20 Ma, K., Pan, X., Fan, P., He, Y., Gu, J., Wang, W., Zhang, T., Li, Z., and Luo, X. (2014). Loss of miR-638 in vitro promotes cell invasion and a mesenchymal-like transition by influencing SOX2 expression in colorectal carcinoma cells. Mol. Cancer 13, 118.   DOI
21 Ojha, R., Jha, V., Singh, S.K., and Bhattacharyya, S. (2014). Autophagy inhibition suppresses the tumorigenic potential of cancer stem cell enriched side population in bladder cancer. Biochim. Biophys. Acta. 1842, 2073-2086.   DOI
22 Ozen, M., Karatas, O.F., Gulluoglu, S., Bayrak, O.F., Sevli, S., Guzel, E., Ekici, I.D., Caskurlu, T., Solak, M., Creighton, C.J., et al. (2015). Overexpression of miR-145-5p inhibits proliferation of prostate cancer cells and reduces SOX2 expression. Cancer Invest. 33, 251-258.   DOI
23 Park, D., Park, H., Kim, Y., Kim, H., and Jeoung, D. (2014a). HDAC3 acts as a negative regulator of angiogenesis. BMB Rep. 47, 227-232.   DOI
24 Park, H., Kim, Y., Park, D., and Jeoung, D. (2014b). Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1. BMB Rep. 47, 342-347.   DOI
25 Yang, P., Huo, Z., Liao, H., and Zhou, Q. (2015). Cancer/testis antigens trigger epithelial-mesenchymal transition and genesis of cancer stem-like cells. Curr. Pharm. Des. 21, 1292-1300.   DOI
26 Por, E., Byun, H.J., Lee, E.J., Lim, J.H., Jung, S.Y., Park, I., Kim, Y.M., Jeoung, D.I., and Lee, H. (2010). The cancer/testis antigen CAGE with oncogenic potential stimulates cell proliferation by up-regulating cyclins D1 and E in an AP-1- and E2F-dependent manner. J. Biol. Chem. 285, 14475-14485.   DOI
27 Shankar, S., Nall, D., Tang, S.N., Meeker, D., Passarini, J., Sharma, J., and Srivastava, R.K. (2011). Resveratrol inhibits pancreatic cancer stem cell characteristics in human and KrasG12D transgenic mice by inhibiting pluripotency maintaining factors and epithelialmesenchymal transition. PLoS One 6, e16530.   DOI
28 Wang, Y., Tian, Y., Morley, M.P., Lu, M.M., Demayo, F.J., Olson, E.N., and Morrisey, E.E. (2013). Development and regeneration of Sox2+ endoderm progenitors are regulated by a Hdac1/2-Bmp4/Rb1 regulatory pathway. Dev. Cell 24, 345-358.   DOI
29 Yin, B., Zeng, Y., Liu, G., Wang, X., Wang, P., and Song, Y. (2014). MAGE-A3 is highly expressed in a cancer stem cell-like side population of bladder cancer cells. Int. J. Clin. Exp. Pathol. 7, 2934-2941.