Browse > Article

An Arabidopsis Homologue of Human Seven-in-Absentia-interacting Protein Is Involved in Pathogen Resistance  

Kim, Youn-Sung (Molecular Signaling Laboratory, Department of Chemistry, Seoul National University)
Ham, Byung-Kook (School of Life Sciences and Biotechnology, Korea University)
Paek, Kyung-Hee (School of Life Sciences and Biotechnology, Korea University)
Park, Chung-Mo (Molecular Signaling Laboratory, Department of Chemistry, Seoul National University)
Chua, Nam-Hai (Laboratory of Plant Molecular Biology, Rockefeller University)
Publication Information
Molecules and Cells / v.21, no.3, 2006 , pp. 389-394 More about this Journal
Abstract
Human seven-in-absentia (SIAH)-interacting protein (SIP) is a component of the E3 ligase complex targeting beta-catenin for destruction. Arabidopsis has one SIP protein (AtSIP) with 32% amino acid sequence identity to SIP. To investigate the functions of AtSIP, we isolated an atsip knockout mutant, and generated transgenic plants overexpressing AtSIP. The growth rates and morphologies of the atsip and transgenic plants were indistinguishable from those of wild type. However, atsip plants were more susceptible to Pseudomonas syringae infection, and the transgenic plants overexpressing AtSIP were more resistant. Consistent with this, RNA blot analysis showed that the AtSIP gene is strongly induced by wounding and hydrogen peroxide treatment. In addition, when plants were infected with P. syringae, AtSIP was transiently induced prior to PR-1 induction. These observations show that Arabidopsis AtSIP plays a role in resistance to pathogenic infection.
Keywords
Arabidopsis thaliana; Biotic Stress; Disease Resistance; Hydrogen Peroxide; SIAH Interacting Protein; Wounding;
Citations & Related Records

Times Cited By Web Of Science : 3  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Austin, M. J., Muskett, P., Kahn, K., Feys, B. J., Jones, J. D. G., et al. (2002) Regulatory role of SGT1 in early R genemediated plant defenses. Science 295, 2077-2080   DOI   ScienceOn
2 Germani, A., Bruzzoni-Giovanelli, H., Fellous, A., Gisselbrecht, S., Varin-Blank, N., et al. (2000) SIAH-1 interacts with alpha- tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis. Oncogene 19, 5997-6006   DOI   ScienceOn
3 Grant, J. J. and Loake, G. J. (2000) Role of ROIs and cognate redox signaling in disease resistance. Plant Physiol. 124, 21-29   DOI   ScienceOn
4 House, C. M., Frew, I. J., Huang, H. L., Wiche, G., Traficante, N., et al. (2003) A binding motif for Siah ubiquitin ligase. Proc. Natl. Acad. Sci. USA 100, 3101-3106   DOI   ScienceOn
5 Kost, B., Spielhofer, P., and Chua, N. H. (1998) A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes. Plant J. 16, 393-401   DOI   ScienceOn
6 Park, C. Y., Heo, W. D., Yoo, J. H., Lee, J. H., Kim, M. C., et al. (2004) Pathogenesis-related gene expression by specific calmodulin isoforms is dependent on NIM1, a key regulator of systemic acquired resistance. Mol. Cells 18, 207-213
7 Sisini, L., Passer, B. J., Amzallag-Elbaz, N., Juven-Gershon, T., Prieur, S., et al. (2001) Siah-1 binds and regulates the function of Numb. Proc. Natl. Acad. Sci. USA 98, 15067-15072   DOI   ScienceOn
8 Tanikawa, J., Ichikawa-Iwata, E., Kanei-Ishii, C., Nakai, A., Matsukawa, S. I., et al. (2000) p53 suppresses the c-Mybinduced activation of heat shock transcription factor 3. J. Biol. Chem. 275, 15578-15585   DOI   ScienceOn
9 Klüsener, B., Young, J. J., Murata, Y., Allen, G. J., Mori, I. C., et al. (2002) Convergence of calcium signaling pathways of pathogenic elicitors and abscisic acid in Arabidopsis guard cells. Plant Physiol. 130, 2152-2163   DOI   ScienceOn
10 Park, T. J., Hamanaka, H., Ohshima, T., Watanabe, N., Mikoshiba, K., et al. (2003) Inhibition of ubiquitin ligase Siah-1A by disabled-1. Biochem. Biophys. Res. Commun. 302, 671- 678   DOI   ScienceOn
11 Zhang, J., Guenther, M. G., Carthew, R. W., and Lazar, M. A. (1998) Proteasomal regulation of nuclear receptor corepressor- mediated repression. Genes Dev. 12, 1775-1780   DOI   ScienceOn
12 Filipek, A., Jastrzebska, B., Nowotny, M., Kwiatkowska, K., Hetman, M., et al. (2002) Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuoblastoma NB-2a cells. J. Biol. Chem. 277, 21103-21109   DOI   ScienceOn
13 Levine, A., Pennell, R. I., Alvarez, M. E., Palmer, R., and Lamb, C. (1996) Calcium-mediated apoptosis in a plant hypersensitive disease resistance response. Curr. Biol. 6, 427-437   DOI   ScienceOn
14 Germani, A., Romero, F., Houlard, M., Camonis, J., Gisselbrecht, S., et al. (1999) hSiah2 is a new Vav binding protein which inhibits Vav-mediated signaling pathways. Mol. Cell Biol. 19, 3798-3807   DOI
15 Kuo, H. F., Tsai, Y. F., Young, L. S., and Lin, C. Y. (2000) Ethanol treatment triggers a heat shock-like response but no thermotolerance in soybean (Glycine max cv. Kaohsiung No. 8) seedlings. Plant Cell Environ. 23, 1099-1108   DOI   ScienceOn
16 Maffei, M., Bossi, S., Spiteller, D., Mithöfer, A., and Boland, W. (2004) Effects of feeding Spodoptera littoralis on lima bean leaves. I. membrane potentials, intracellular calcium variations, oral secretions, and regurgitate components. Plant Physiol. 134, 1752-1762   DOI   ScienceOn
17 Bursen, A., Moritz, S., Gaussmann, A., Dingermann, T., and Marschalek, R. (2004) Interaction of AF4 wild-type and AF4.MLL fusion protein with SIAH proteins: indication for t(4;11) pathobiology. Oncogene 23, 6237-6249   DOI   ScienceOn
18 DeMoraes, C. M., Lewis, W. J., Paré, P. W., Alborn, H. T., and Tumlinson, J. H. (1998) Hebivore-infested plants selectively attract parasitoids. Nature 393, 570-573   DOI   ScienceOn
19 Moller, S. G., Kim, Y. S., Kunkel, T., and Chua, N. H. (2003) PP7 is a positive regulator of blue light signaling in Arabidopsis. Plant Cell 15, 1111-1119   DOI   ScienceOn
20 Matsuzawa, S., Takayama, S., Froesch, B., Zapata, J. M., and Reed, J. C. (1998) p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1. EMBO J. 17, 2736-2747   DOI   ScienceOn
21 Jabs, T., Tschope, M., Colling, C., Hahlbrock, K., and Scheel, D. (1997) Elicitor-stimulated ion fluxes and O2- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsely. Proc. Natl.Acad. Sci. USA 94, 4800-4805   DOI   ScienceOn
22 Matsuzawa, S. and Reed, J. C. (2001) Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses. Mol. Cell 7, 915-926   DOI   ScienceOn
23 Clough, S. J. and Bent, A. F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arbidopsis thaliana. Plant J. 16, 735-743   DOI   ScienceOn
24 Gray, W. M., Muskett, P. R., Chuang, H., and Parker, J. E. (2003) Arabidopsis SGT1b is required for SCFTIR1-mediated auxin response. Plant Cell 15, 1310-1319   DOI   ScienceOn
25 Hui, D., Iqbal, J., Lehmann, K., Gase, K., Saluz, H. P., et al. (2003) Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural Nicotiana attenuate. V. Microarray analysis and further characterization of large scale changes in heibivore-induced mRNAs. Plant Physiol. 131, 1877-1893   DOI   ScienceOn
26 Oliver, P. L., Bitoun, E., Clark, J., Jones, E. L., and Davies, K. E. (2004) Mediation of Af4 protein function in the cerebellum by Siah proteins. Proc. Natl. Acad. Sci. USA 101, 14901- 14906   DOI   ScienceOn
27 Santelli, E., Leone, M., Li, C., Fukushima, T., Preece, N. E., et al. (2005) Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex. J. Biol. Chem. 280, 34278-34287   DOI   ScienceOn
28 Song, J. T., Lu, H., McDowell, J. M., and Greenberg, J. T. (2004) A key role for ALD1 in activation of local and systemic defenses in Arabidopsis. Plant J. 40, 200-212   DOI   ScienceOn
29 Orozco-Cardenas, M. and Ryan, C. A. (1999) Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proc. Natl. Acad. Sci. USA 96, 6553-6557   DOI   ScienceOn
30 Xie, Q., Guo, H. S., Dallman, G., Fang, S., Weissman, A. M., et al. (2002) SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals. Nature 419, 167-170   DOI   ScienceOn
31 Hu, G., Chung, Y. L., Glover, T., Valentine, V., Look, A. T., et al. (1997) Characterization of human homologs of the Drosophila seven in absentia (sina) gene. Genomics 46, 103-111   DOI   ScienceOn
32 Tor, M., Gordon, P., Cuzick, A., Eulgem, T., Sinapidou, E., et al. (2002) Arabidopsis SGT1b is required for defense signaling conferred by several downy mildew resistance genes. Plant Cell 14, 993-1003   DOI   ScienceOn