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Permeabilization of Ochrobactrum anthropi SY509 Cells with Organic Solvents for Whole Cell Biocatalyst  

Park, Kyung-Oh (School of Chemical Engineering, Seoul National University)
Song, Seung-Hoon (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University)
Yoo, Young-Je (School of Chemical Engineering, Seoul National University, Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.3, 2004 , pp. 147-150 More about this Journal
Abstract
Permeabilization is known to overcome cell membrane barriers of whole cell biocatalysts. The use of organic solvents is advantageous in terms of cost, simplicity, and efficiency. In this study, Ochrobactrum anthropi SY509 was permeabilized with various organic solvents. Treatment with organic solvents resulted in lower permeability barriers due to falling out lipids of the cell membrane. Therefore, permeabilized cells showed higher enzyme activity with no cell viability. Among various organic solvents, 0.5% (v/v) chloroform was selected as the most efficient permeabilizing reagent. Changes in the cell membrane structure were observe d and the residual amounts of phospholipids of the cell membrane were measured to investigate the mechanism of the improved permeability.
Keywords
permeabilization; permeability barrier; organic solvent; Ochrobactrum anthropi; phospholipids;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 13  (Related Records In Web of Science)
Times Cited By SCOPUS : 12
연도 인용수 순위
1 Studies on lactate dehydrogenase of Lactobacillus plantarum spp. involved in lactic acid biosynthesis using permeabilized cells /
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2 Permeabilization of Yarrowia lipolytica cells by triton X-100 /
[ Galabova,D.;B.Tuleva;D.Spasova ] / Enzyme Microb. Technol.   DOI   ScienceOn
3 Effect of Aeration on Denitrification by Ochrobactrum anthropi SY509 Biotechnol /
[ Song,S.H.;S.H.Yeom;S.S.Choi;Y.J.Yoo ] / Bioprocess Eng.   과학기술학회마을   DOI
4 Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized with organic solvents /
[ e Leon,A.;B.Garcia;A.P.Barba de la Rosa;F.Villasenor;A.Estrada;R.Lopez-Revilla ] / Proc. Biochem.
5 Production of D-amino acid precursors with permeabilized recombinant Escherichia coli with D-hydantoinase activity /
[ Yin,B.;Y.hen;S.Lin;W.Hsu ] / Process Biochem.   DOI
6 /
[ Knowles,R. ] / Denitrification. Microbiol. Rev.
7 Dependence of yeast permeabilization with Triton X-100 on the cell age /
[ Christova,N.;B.Tuleva;D.Galabova ] / Biotechnol. Tech.   DOI
8 /
[ Felix,H. ] / Bioconversions in Permeabilized Cells
9 Permeabilization of yeast cells (Kluyveromyces lactis) with organic solvents /
[ Flores,M.V.;C.E.Voget;R.J.J.Ertola ] / Enzyme Microb. Technol.   DOI   ScienceOn
10 A study on the denitrification characteristics of permeabilized Paracoccus denitrificans /
[ Song,J.Y.;S.Y.Hwang;D.S.Kim ] / Kor. J. Biotechnol. Bioeng.   과학기술학회마을
11 Isolation and characterization of nitrate reductase from Escherichia coli. /
[ McGregor,C.H. ] / Methods Enzymol.   DOI
12 Bioelectrochemical denitrification by Pseudomonas sp. or anaerobic bacterial consortium /
[ Park,D.H.;Y.K.Park ] / J. Microbiol. Biotechnol.   과학기술학회마을
13 A rapid method of total lipid extraction and purification /
[ Bligh,E.G.;W.J.Dyer ] / Can. J. Biochem. Physiol.   DOI
14 Colorimetric determination of phospholipids with ammonium ferrothiocyanate /
[ Stewart,J.C.M. ] / Anal. Biochem.   DOI   ScienceOn
15 Preparation of high-activity whole cell biocatalysts by permeabilization of recombinant yeasts with alcohol /
[ Liu,Y.;Y.Fujita;A.Kondo;H.Fukuda ] / J. Biosci. Bioeng.   DOI   ScienceOn
16 Reduction of nitrate and nitrite in water by immobilized enzymes /
[ Mellor,R.;B.Ronnennberg;H.W.Campbell;S.Diekmann ] / Nature   DOI
17 Permeabilized cells /
[ Felix,H. ] / Anal. Biochem.   DOI   ScienceOn
18 Effect of tungsten on nitrate and nitrite reductase in Azospirillum brasilense sp7 /
[ Chauret,C.;R.Knowles ] / Can. J. Microbiol.   DOI
19 Topology and transport of membrane lipids in bacteria /
[ Richard,P.;H.Huijbregts;de Kroon A.I.P.M.;B. de Kruijff ] / Biochim. Biophys. Acta.   DOI   ScienceOn
20 Comparison of various permeabilization treatments on Kluyveromyces by determining in situ β-galactosidase activity /
[ Decleire,M.;W. de Cat;N. van Huynh ] / Enzyme Microb. Technol.   DOI   ScienceOn
21 Effect of oxidation-reduction potential on denitrification by Ochrobactrum anthropy SY509 /
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22 Detergent inhibition of nitric-oxide reductase activity /
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