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http://dx.doi.org/10.3344/kjp.2008.21.3.179

Spinal α2 Adrenoceptor and Antiallodynic Effect by Clonidine after Chronic Administration of 4-Methylcatechol in Neuropathic Rat Pain Model  

Chung, Kyu Yeon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
Shin, Sang Wook (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
Choi, Bong Soo (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
Kim, Chul Hong (Department of Anesthesia and Pain Medicine, School of Dentistry, Pusan National University)
Kim, Kyung Hoon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
Kim, Hae Kyu (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
Publication Information
The Korean Journal of Pain / v.21, no.3, 2008 , pp. 179-186 More about this Journal
Abstract
Background: The adrenergic nervous system in the spinal cord contributes to the development of neuropathic pain after nerve injury. Brain derived neurotrophic factor may facilitate the sympathetic change in the spinal cord and influence the state of neuropathic pain. We probed the effect of chronic repetitive administration of systemic 4-methylcatechol, which is known to be a neurotrophic factor inducer, in a spinal nerve ligation model. Methods: We made the rat neuropathic pain model by the ligation of the L5 spinal nerve. Intraperitoneal 4-methylcatechol ($10{\mu}g/kg$) or the same volume of saline wasadministrated twice daily just after the operation for 7 days. The tactile allodynia was measured by using von Frey filaments and its change was followed up from 3 days after SNL. The lumbosacral enlargement of the spinal cord was taken out and the mRNA contents of the ${\alpha}_2-adrenoceptor$ subtypes were measured by real time polymerase chain reaction and this was then compared with the control groups. The antiallodynic effect of intrathecal clonidine (3, 10, $30{\mu}g$) was evaluated and compared in the 4-methylcatechol treated rats and the control rats. Results: The expression of the ${\alpha}_{2A}$ and ${\alpha}_{2C}$ adrenoceptor subtypes did not change after 4-methylcatechol treatment. Intrathecal clonidine showed an earlier and better effect at the highest dose ($30{\mu}g$ intrathecal), but not with any other doses. Conclusions: Chronic intraperitoneal administration of 4-methylcatechol may improve the effect of intrathecal clonidine, but we could not prove the increase of ${\alpha}_{2A}$ and ${\alpha}_{2C}$ adrenoceptors in the spinal cord of 4-methylcatechol treated rats.
Keywords
adrenergic receptor; brain derived neurotrophic factor; neuropathic pain; spinal nerve ligation; 4-methylcatechol;
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Times Cited By KSCI : 1  (Citation Analysis)
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