Browse > Article

The Comparative Study between PLGA and Chitosan Scaffolds for Cartilage Tissue Engineering  

Lee, Yong Jik (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Chung, Ho Yun (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Shin, Dong Phil (Dr. Shin's Aesthetic Plastic Surgical Clinic)
Kim, Jong Yeop (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Yang, Jung Duk (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Lee, Dong Gul (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Park, Jae Woo (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Cho, Byung Chae (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital)
Baik, Bong Soo (Department of Plastic & Reconstructive Surgery, Ulsan Dongkang Hospital)
Publication Information
Archives of Plastic Surgery / v.32, no.5, 2005 , pp. 599-606 More about this Journal
Abstract
Clinical application of the cartilage formed by tissue engineering is of no practical use due to the failure of long-term structural integrity maintenance. One of the important factors for integrity maintenance is the biomaterial for a scaffold. The purpose of this study is to evaluate the difference between polylactic-co-glycolic acids (PLGA) and chitosan as scaffolds. Human auricular chondrocytes were isolated, cultured, and seeded on the scaffolds, which were implanted in the back of nude mice. Eight animals were sacrificed at 4, 8, 12, 16, and 24 weeks after implantation respectively. In gross examination and histological findings, the volume of chondrocyte-PLGA complexes was decreased rapidly. The volume of chondrocyte-chitosan complexes was well maintained with a slow decrease rate. The expression of type II collagen protein detected by immunohistochemistry and western blots became weaker with time in the chondrocyte-PLGA complexes. However, the expression in the chondrocyte-chitosan complexes was strong for the whole period. Collagen type II gene expressions using RT-PCR showed a similar pattern. In conclusion, these results suggest that chitosan is a superior scaffold in cartilage tissue engineering in terms of structural integrity maintenance. It is expected that chitosan scaffold may become one of the most useful scaffolds for cartilage tissue engineering.
Keywords
PLGA; Chitosan; Scaffold; Cartilage tissue engineering;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chalain TD, Philips JH, Hinek A: Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and x-elastin. J Biomed Mater Res 44: 280, 1999   DOI   ScienceOn
2 Kim WS, Vacanti JP, Cima L, Mooney D, Upton J, Puelacher WC, Vacanti CA: Cartilage engineered in predetermined shapes employing cell transplantation on synthetic biodegradable polymers. Plast Reconstr Surg 94: 233, 1994   DOI   ScienceOn
3 Dessau W, Sasse J, Timpl R, Jilek F, von der Mark K: Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. J Cell Biol 79: 342, 1978   DOI
4 Glowacki J, Trepman E, Folkman J: Cell shape and phenotypic expression in chondroeytes. Proc Soc Exp Biol Med 172: 93, 1983
5 Saadeh PB, Brent B, Mehrara BJ, Steinbrech DS, Ting V, Gittes GK, Longaker MT: Human cartilage engineering: chondrocyte extraction, proliferation, and characterization for construct development. Ann Plast Surg 42: 509, 1999   DOI   ScienceOn
6 Risbud MV, Sittinger M: Tissue engineering: advances in vitro cartilage generation. Trends Biotechnol 20: 351, 2002   DOI   ScienceOn
7 Ma HL, Hung SC, Lin SY, Chen YL, Lo WH: Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads. J Biomed Mater Res 64A: 273, 2003   DOI
8 Bahrami S, Stratmann U, Wiesmann HP, Mokrys K, Bruckner P, Szuwart T: Periosteally derived osteoblast-like cells differentiate into chondrocytes in suspension culture in agarose. Anat Rec 259: 124, 2000   DOI   ScienceOn
9 Vacanti CA, Langer R, Schloo B, Vacanti JP: Synthetic polymers seeded with chondrocytes provide a template for new cartilage formation. Plast Reconstr Surg 88: 753, 1991   DOI   ScienceOn
10 Cao Y, Vacanti JP, Paige KT, Upton J, Vacanti CA: Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear. Plast Reconstr Surg 100: 297, 1997   DOI   ScienceOn
11 Zalzal GH, Cotton RT, McAdams AJ: Cartilage grafts-present status. Head Neck Surg 8: 363, 1986   DOI   ScienceOn
12 Tomihata K, Ikada Y: In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials 18: 567, 1997   DOI   ScienceOn
13 Rodriguez A, Cao YL, Ibarra C, Pap S, Vacanti M, Eavery RD, Vacanti CA: Characteristics of cartilage engineered from human pediatric auricular cartilage. Plast Reconstr Surg 103: 1111, 1999   DOI   ScienceOn
14 Vacanti JP: Beyond transplantation. Arch Surg 123: 545, 1988   DOI   ScienceOn