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The Effect of Prostaglandin E1 on Apoptosis Induced by Ischemia Reperfusion Injury in Rat Intestinal Mucosa  

Bae, Tae Hui (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University)
Kim, Seung Hong (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University)
Kim, Cheol Kyu (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University)
Kim, Han Koo (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University)
Kim, Woo Seob (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University)
Publication Information
Archives of Plastic Surgery / v.32, no.3, 2005 , pp. 369-375 More about this Journal
Abstract
Apoptosis is a physiologic or programmed cell death process which is controlled by genes. It is essential for the function and the appropriate development of multicellular organism. It is also thought to be one of the main mechanisms of cell death in ischemic tissues. The effect of prostaglandin $E_1$($PGE_1$) is proven to be useful in the recovery of ischemic changes by inducing vasodilation of peripheral vessels and platelet disaggregation. $PGE_1$ is also known to suppress apoptosis in human liver sinusoidal endothelial cell from ischemia-reperfusion injury. The purpose of this study is to evaluate the effects of $PGE_1$ on the apoptosis in the ischemia reperfusion injury of rat intestine. Thirty Sprague-Dawley rats were used. In control group(N=15), superior mesenteric artery was occluded for 60 minutes and after removing the vessel clamp, it was reperfused for 60 minutes and harvested. In experimental group(N=15), a jejunal flap was also made as in the control group except for the intraarterial administration of the $PGE_1$ right after clamping the artery and removing the clamp. H&E, TUNEL and immunohistochemical stains for p53, bax, and bcl-2 were performed. There were ischemic changes in gross and microscopic findings in both groups. The apoptotic index was significantly lower in the experimental group($1.29{\pm}0.82$(p=0.003)) than in the control group ($2.33{\pm}0.95$). The rat intestinal ischemia apoptosis by ischemia-reperfusion was partly related to the modulating of bcl-2, bax, and p53 expression. Our results indicate that $PGE_1$ suppresses the apoptosis in the ischemic jejunal flap and this effect is probably the result of a increase in expression of bcl-2.
Keywords
Apoptosis; Prostaglandin $E_1$; Intestines; Ischemia- reperfusion injury;
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1 Tsuchida Y, Aoki N, Fukuda O, Nakano M: Changes in hemodynamics in jejunal flaps of rabbits due to ischemia, venous congestion, and reperfusion as measured by means of colored microspheres. Plast Reconstr Surg 101: 147, 1998   DOI   ScienceOn
2 Sohn WR, Lee JW, Burm JS, Chung CH, Oh SJ: The effect of $PGE_1$ on the skin flap survival after secondary venous ischemia. J Korean Soc Plast Reconstr Surg 24: 651, 1997
3 Fukuda K, Kojiro M, Chiu JF: Demonstration of extensive chromatin cleavage in transplatated Morris hepatoma 7777 tissue: apoptosis or necrosis? Am J Pathol 142: 935, 1993
4 Olding M, Jeng JC: Ischemic tolerance of canine jejunal flaps. Plast Reconstr Surg 94: 167, 1994   DOI   ScienceOn
5 Hill IE, MacManus JP, Rasquinha I, Tuor UI: DNA fragmentation indicative of apoptosis following unilateral cerebral hypoxia-ischemia in the neonatal rat. Brain Res 676: 398, 1995   DOI   ScienceOn
6 Itoh G, Tamura M. Suzuki Y: DNA fragmentation infarcted myocardial cells demonstrated by the nick end labelling method and DNA agarose gel electrophoresis. Am J Pathol 146: 1325, 1995
7 Zhang Y, Wu YX, Hao YB, Dun Y, Yang SP: Role of endogenous opioid peptides in protection of ischemic preconditoning in rat small intestine. Life Sci 68: 1013, 2001   DOI   ScienceOn
8 Hong Y, Pietro M, Philippe M, Christian T, Frederic T, Jose' O, Gilles M, Jinning L: Prostaglandin E1 protects human liver sinusoidal endothelial cell from apoptosis induced by hypoxia reoxygenation. Microvas Resear 64: 94, 2002   DOI   ScienceOn
9 Hassoun HT, Zou L, Moore FA: Alpha-melanocyte-stimulating hormone protects against mesenteric ischemia-reperfusion injury. Am J Physiol Gastrointest Liver Physiol 282: 1059, 2002   DOI
10 Liu H, McPherson BC, Yao Z: Preconditioning attenuates apoptosis and necrosis: Role of protein kinase C$\epsilon$ and -$\delta$ isoforrns. Am J Physiol Heart Circ Physiol 281: 404, 2001   DOI
11 Kojima M, Iwakiri R. Wu B, Fujise T, Watanabe K, Lin T, Amemori S, Sakata H, Shimoda R, Oguzu T, Ootani A, Tunada S, Fujimoto K: Effects of antioxidative agents on apoptosis induced by ischemia-reperfusion in rat intestinal mucosa. Aliment Pharmacol Ther 18: 139, 2003   DOI   ScienceOn
12 Web SJ, Harrison DJ, Wyllie AH: Apoptoeis, An overview of the process and its relavance in disease. Kaufmann SH (editor): Apoptosis, California, Academic Press, 1997, p 1
13 Noda T, Iwakiri R, Fujimoto K. Matsuo S, Aw TY: Programmed cell death induced by ischemia-reperfusion in rat intestinal mucosa. Am J Physiol 274: G270, 1998