[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5487/TR.2013.29.2.099

In Vitro Evaluation of Antimicrobial Activity of Lactic Acid Bacteria against Clostridium difficile

Lee, Joong-Su (R&D Center, CellBiotech, Co. Ltd.)
Chung, Myung-Jun (R&D Center, CellBiotech, Co. Ltd.)
Seo, Jae-Gu (R&D Center, CellBiotech, Co. Ltd.)

Publication Information

Toxicological Research / v.29, no.2, 2013 , pp. 99-106 More about this Journal

Abstract

Clostridium difficile infection (CDI) has become a significant threat to public health. Although broad-spectrum antibiotic therapy is the primary treatment option for CDI, its use has evident limitations. Probiotics have been proved to be effective in the treatment of CDI and are a promising therapeutic option for CDI. In this study, 4 strains of lactic acid bacteria (LAB), namely, Lactobacillus rhamnosus (LR5), Lactococcuslactis (SL3), Bifidobacterium breve (BR3), and Bifidobacterium lactis (BL3) were evaluated for their anti-C. difficile activity. Co-culture incubation of C. difficile ( $10^6$ and $10^{10}$ CFU/ml) with each strain of LAB indicated that SL3 possessed the highest antimicrobial activity over a 24-hr period. The cell-free supernatants of the 4 LAB strains exhibited $MIC_{50}$ values between 0.424 mg/ml (SL3) and 1.318 (BR3) mg/ml. These results may provide a basis for alternative therapies for the treatment of C. difficile-associated gut disorders.

Keywords

Clostridium difficile infection; lactic acid bacteria; antimicrobial activity; cell-free supernatant;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

Reference
Cited By KSCI

1	Mulligan, M.E., Rolfe, R.D., Finegold, S.M. and George, W.L. (1979) Contamination of a hospital environment by Clostridium difficile. Curr. Microbiol., 3, 173-175. DOI
2	Nelson, R. (2007) Antibiotic treatment for Clostridium difficile- associated diarrhea in adults. Cochrane Database Syst. Rev., 18, CD004610.
3	Bartlett, J.G. (2010) Clostridium difficile: progress and challenges. Ann. N. Y. Acad. Sci., 1213, 62-69. DOI ScienceOn
4	Ohl, M.E., Stevermer, J.J., Meadows, S., Tribuna, J. and Chek, K. (2005) What are effective therapies for Clostridium difficile-associated diarrhea? J. Fam. Pract., 54, 176-178.
5	Dethlefsen, L., Huse, S., Sogin, M.L. and Relman, D.A. (2008) The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol., 6, e280. DOI ScienceOn
6	Kang, B.S., Seo, J.G., Lee, G.S., Kim, J.H., Kim, S.Y., Han, Y.W., Kang, H., Kim, H.O., Rhee, J.H., Kang, B.S., Seo, J.G., Lee, G.S., Kim, J.H., Kim, S.Y., Han, Y.W., Kang, H., Kim, H.O., Rhee, J.H., Chung, M.J. and Park, Y.M. (2009) Antimicrobial activity of enterocins from Enterococcus faecalis SL-5 against Propionibacterium acnes, the causative agent in acne vulgaris, and its therapeutic effect. J. Microbiol., 47, 101-109. 과학기술학회마을 DOI ScienceOn
7	Forestier, C., De Champs, C., Vatoux, C. and Joly, B. (2001) Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties. Res. Microbiol., 152, 167-173. DOI ScienceOn
8	Guarner, F. and Schaafsma, G.J. (1998) Probiotics. Int. J. Food Microbiol., 39, 237-238. DOI ScienceOn
9	Jin, L.Z., Ho, Y.W. Abdullah, N., Ali, M.A. and Jalaludin, S. (1996) Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken. Lett. Appl. Microbiol., 23, 67-71. DOI ScienceOn
10	Guandalini, S. (2006) Probiotics for children: use in diarrhea. J. Clin. Gastroenterol., 40, 244-248. DOI ScienceOn
11	Katz, J.A. (2006) Probiotics for the prevention of antibioticassociated diarrhea and Clostridium difficile diarrhea. J. Clin. Gastroenterol., 40, 249-255. DOI ScienceOn
12	Fooks, L.J., Fuller, R. and Gibson, G.R. (1999) Prebiotics, probiotics and human gut microbiology. Int. Dairy J., 9, 53-61. DOI ScienceOn
13	Trejo, F.M., Perez, P.F. and De Antoni, G.L. (2010) Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro. Antonie van Leeuwenhoek, 98, 19-29. DOI ScienceOn
14	Cha, B.K., Jung, S.M., Choi, C.H., Song, I.D., Lee, H.W., Kim, H.J., Hyuk, J., Chang, S.K., Kim, K., Chung, W.S. and Seo, J.G. (2012) The effect of a multispecies probiotic mixture on the symptoms and fecal microbiota in diarrhea-dominant irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. J. Clin. Gastroenterol., 46, 220-227. DOI ScienceOn
15	Clinical and Laboratory Standards Institute. (2006) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically (7th ed.), Clinical and Laboratory Standards Institute, Wayne, PA, pp. 1-49.
16	Folkers, B.L., Schuring, C., Essmann, M. and Larsen, B. (2010) Quantitative real time PCR detection of Clostridium difficile growth inhibition by probiotic bacteria. N. Am. J. Med. Sci., 2, 5-10.
17	Gonzalez, L., Sandoval, H., Sacristan, N., Castro, J.M., Fresno, J.M. and Tornadijo, M.E. (2007) Identification of lactic acid bacteria isolated from Genestoso cheese throughout ripening and study of their antimicrobial activity. Food Control, 18, 716-722. DOI ScienceOn
18	Bartoloni, A., Mantella, A., Goldstein, B.P., Dei, R., Benedetti, M., Sbaragli, S. and Paradisi, F. (2004) In-vitro activity of nisin against clinical isolates of Clostridium difficile. J. Chemother., 16, 119-121. DOI ScienceOn
19	Kojic, M., Svicevic, J., Banina, A. and Topisirovic, L. (1991) Bacteriocin-producing strain of Lactococcus lactis subsp. diacitilactis S50. Appl. Environ. Microbiol., 57, 1835-1837.
20	Kozak, W., Bardowski, J. and Dobrzansky, W.T. (1978) Lactostrepcins- acid bacteriocin produced by lactic streptococci. J. Dairy Res., 45, 247-257. DOI ScienceOn
21	De Vuyst, L. (1994) Nisin production variability between natural Lactococcus lactis subsp. Lactis strains. Biotechnol. Lett., 16, 287-292. DOI
22	Pochapin, M. (2000) The effect of probiotics on Clostridium difcile diarrhea. Am. J. Gastroenterol., 95, S11-S13. DOI ScienceOn
23	Aroutcheva, A., Gariti, D., Simon, M., Shott, S., Faro, J., Simoes, J.A., Gurguis, A. and Faro, S. (2001) Defense factors of vaginal lactobacilli. Am. J. Obstet. Gynecol., 185, 375-379. DOI ScienceOn
24	Surawicz, C.M., McFarland, L.V., Greenberg, R.N., Rubin, M., Fekety, R., Mulligan, M.E., Garcia, R.J., Brandmarker, S., Bowen, K., Borjal, D. and Elmer, G.W. (2000) The search for a better treatment for recurrent Clostridium difcile disease: use of high-dose vancomycin combined with Saccharomyces boulardii. Clin. Infect. Dis., 31, 1012-1017. DOI ScienceOn
25	McFarland, L.V., Surawicz, C.M., Greenberg, R.N., Fekety, R., Elmer, G.W., Moyer, K.A., Melcher, S.A., Bowen, K.E., Cox, J.L., Noorani, Z., Harrington, G., Rubin, M. and Greenwald, D. (1994) A randomized placebo-controlled trial of Saccharomyces boulardii in combination with standard antibiotics for Clostridium difcile disease. JAMA, 271, 1913-1918. DOI ScienceOn
26	Wullt, M., Hagslatt, M.L. and Odenholt, I. (2003) Lactobacillus plantarum 299v for the treatment of recurrent Clostridium difcile-associated diarrhoea: a double-blind, placebo-controlled trial. Scand. J. Infect. Dis., 35, 365-367. DOI ScienceOn
27	Lawrence, S.J., Korzenik, J.R. and Mundy, L.M. (2005) Probiotics for recurrent Clostridium difcile disease. J. Med. Microbiol., 54, 905-906. DOI ScienceOn
28	Aslam, S., Hamill, R.J. and Musher, D.M. (2005) Treatment of Clostridium difficile-associated disease: old therapies and new strategies. Lancet Infect. Dis., 5, 549-557. DOI ScienceOn

5	Sabina Fijan. (2014) International Journal of Environmental Research and Public Health Microorganisms with Claimed Probiotic Properties: An Overview of Recent Literature / 11 (5) , 4745
1	Hyunho Yoon. (2014) Toxicological Research A Probiotic Preparation Duolac-Gold Ameliorates Dextran Sulphate Sodium-induced Mouse Colitis by Downregulating the Expression of IL-6 / 30 (1) , 27
30	Tatsinkou Fossi Bertrand. (2016) African Journal of Microbiology Research Lactic acid bacteria from traditionally processed corn beer and palm wine against selected food-borne pathogens isolated in south west region of Cameroon / 10 (30) , 1140
1664-302X	L. Valdés-Varela. (2016) Frontiers in Microbiology Effect of Bifidobacterium upon Clostridium difficile Growth and Toxicity When Co-cultured in Different Prebiotic Substrates / 7 (1664-302X)
2050-084X	David M Garcia. (2016) eLife A common bacterial metabolite elicits prion-based bypass of glucose repression / 5 (2050-084X)
1867-1314	(2019) Probiotics and Antimicrobial Proteins Encapsulating Viability of Multi-strain Lactobacilli as Potential Probiotic in Pigs / (1867-1314)
1573-7365	(2018) Metabolic Brain Disease Therapeutic effects of probiotics on neurotoxicity induced by clindamycin and propionic acid in juvenile hamsters / (1573-7365)