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The Effects of Diallyl Disulfide on Antimetastatic Potential of B16-F10 Murine Melanoma Cells  

Kang, Mi-Kyung (Department of Toxicological Research, National Institute of Toxicological Research)
Jun, Hye-Seung (Department of Toxicological Research, National Institute of Toxicological Research)
Yum, Yung-Na (Department of Toxicological Research, National Institute of Toxicological Research)
Hwang, Myung-Sil (Department of Toxicological Research, National Institute of Toxicological Research)
Park, Mi-Sun (Department of Toxicological Research, National Institute of Toxicological Research)
Kim, Ok-Hee (Busan Regional Korea FDA)
Publication Information
Toxicological Research / v.22, no.4, 2006 , pp. 349-356 More about this Journal
Abstract
Diallyl disulfide (DADS), an oil-soluble organosulfur compound in garlic has been reported to suppress tumor growth and to induce apoptosis in cancer. In the present study, we investigated the effects of DADS on pulmonary metastasis of B16-F10 murine melanoma cells. DADS (i.p. 40 mg/kg) significantly (p<0.05) reduced the number of pulmonary metastatic nodules (48%) in experimental pulmonary metastasis assay. We also found that DADS inhibited adhesion, invasion and migration of B16-F10 melanoma cells in a dose-dependent manner. To study the antimetastatic potential of DADS, we performed the effects of DADS on matrix metalloproteinase activity. DADS significantly inhibited the expression of matrix metalloproteinase-2 activity in B16-F10 cells by gelatin zymography. These results suggest that DADS prevent metastasis in part through suppression of migration of B16-F10 melanoma cells by Inhibiting matrix metalloproteirase-2 responsible for degradation of extracellar matrix.
Keywords
Diallyl disulfide; B16F10 melamona cells; Experimental pulmonary metastasis assay; Invasion, Matrix metalloproteinase-2;
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