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http://dx.doi.org/10.17208/kjopp.2014.12.28.6.593

Anti-inflammatory Effect of Euphorbiae kansui Radix Extract in Lipopolysaccharide-stimulated Mouse Peritoneal Macrophages  

Kim, Jeung Beum (Department of Pathology, College of Korean Medicine, Semyung University)
Kyung, Hyuk Su (Department of Pathology, College of Korean Medicine, Semyung University)
Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medicine, KyungHee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.6, 2014 , pp. 593-600 More about this Journal
Abstract
This study is aimed to investigate the anti-inflammatory effect of Euphorbiae kansui radix methanol extract (ERE) in lipopolysaccharide(LPS)-stimulated mouse peritoneal macrophages. Peritoneal macrophages were obtained from thioglycollate-injected Balb/c mice. Cells were stimulated with LPS or LPS plus interferon-gamma (IFN-${\gamma}$) in the presence of ERE and various inflammatory markers were assayed. Finally, LPS-induced signaling molecules were measured. ERE up to $400{\mu}g/m{\ell}$, was not cytotoxic to ERE inhibited LPS/IFN-${\gamma}$-induced nitric oxide (NO), inducible NO synthase. ERE also reduced the levels of cyclooxygenase-2 and the proinflammatory cytokines such as tumor necrosis factor-${\alpha}$, interleukin(IL)-6 and IL-12. The inhibitory effect of ERE on LPS-induced $I{\kappa}B{\alpha}$ degradation was weak but phosphorylation of JNK, p38 and ERK1/2 was strongly suppressed. Our data indicated that the anti-inflammatory effect of ERE in LPS-stimulated macrophages was partly mediated by its inhibition of JNK, p38 and ERK1/2.
Keywords
Euphorbiae kansui radix; anti-inflamatory effect; macrophages; lipopolysaccharide;
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