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Effect of Ethanolic Extract of Silkworm on Reactive Oxygen Species Formation in vivo  

Kang, Young Kook (College of Liberal Arts, Daejeon University)
Choi, Min Joo (Department of Biology, College of Natural Science, Daejeon University)
Nam, Sang Ho (Department of Biology, College of Natural Science, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.4, 2014 , pp. 379-383 More about this Journal
Abstract
The purpose of this study is to investigate the effects of silkworm extracts (SWE) on reactive oxygen species formation in mice (C57BL/6). Mice were administrated intraperitoneally with SWE (20 mg/kg/day) for 14 days. All animals were sacrificed 24 hours after the last SWE treatment and then extracted the blood and brain tissue in mouse. The researcher measured several parameters related to reactive oxygen species formation, malondialdehyde (MDA) and hydrogen peroxide ($H_2O_2$) contents in serum, whole brain, cerebral cortex and cerebellum. The results showed that MDA content of pre-SWE treatment was decreased significantly in serum, mitochondrial and cytosolic fraction of whole brain and cerebellum (P<0.01). The $H_2O_2$ content of pre-SWE treatment was decreased significantly in mitochondrial fraction of whole brain, cerebral cortex and cerebellum (P<0.01). These results suggest that SWE plays an important role for inhibition of oxidative damage of cells as well as antioxidant effect, aging delay and cells protected from irradiation.
Keywords
$H_2O_2$; silkworm; oxidative damage; antioxidant; MDA;
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