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Effect of Drynariae Rhizoma in RANKL-induced Osteoclast Differentiation  

Kwak, Seong-Cheoul (Department of Anatomy, School of Medicine, Wonkwang University & Institute for Skeletal Disease and Wonkwang Medical Science Institute)
Moon, Seo-Young (Division of Anesthesiology and Pain Medicine, Sanbon Medical Center)
Kwack, Han-Bok (Department of Anatomy, School of Medicine, Wonkwang University & Institute for Skeletal Disease and Wonkwang Medical Science Institute)
Jeon, Byung-Hun (Department of Pathology, College of Korean Medicine & Research Center of Traditional Korean Medicine, Wonkwang University)
Min, Oh-Jae (Department of Anatomy, School of Medicine, Wonkwang University & Institute for Skeletal Disease and Wonkwang Medical Science Institute)
Choi, Min-Kyu (Department of Anatomy, School of Medicine, Wonkwang University & Institute for Skeletal Disease and Institute for Environmental Science)
Kim, Jeong-Joong (Department of Anatomy, School of Medicine, Wonkwang University & Institute for Skeletal Disease and Wonkwang Medical Science Institute)
Jang, Sung-Jo (Department of Neurosurgery, Gunsan Medical Center)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.26, no.4, 2012 , pp. 506-510 More about this Journal
Abstract
Bone homeostasis is regulated by the balance between bone-resorbing osteoclasts and bone-forming osteoblasts. Osteoporosis, rheumatoid arthritis and periodontal disease are related with up-regulated osteoclast formation and its activity. Gol-Swae-Bo(Drynariae Rhizoma) is widely used on skeletal disease. In this study, we sought to examine the effect of Drynariae Rhizoma in RANKL-induced osteoclast differentiation. The extract of Drynariae Rhizoma inhibited RANKL-induced osteoclast differentiation in a dose dependent manner without cytotoxicity. receptor activator of nuclear factor-${\kappa}B$ ligand(RANKL) mediated $I{\kappa}B$ degradation in bone marrow macrophages(BMMs). However, the extract of Drynariae Rhizoma inhibited RANKL induced $I{\kappa}B$ degradation in BMMs. And mRNA expression of OSCAR, TRAP, c-Fos and NFATc1 was suppressed by the extract of Drynariae Rhizoma. Moreover, the extract of Drynariae Rhizoma inhibited the protein expression of NFATc1 and c-Fos induced by RANKL. After all the analysis, these results suggest that Drynariae Rhizoma may be good candidate of medicine in the treatment of bone-related disease.
Keywords
Drynariae Rhizoma; Osteoclast; RANKL; Osteoporosis;
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