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Effects of Styrax Liquides on the Secretion of ${\beta}$-amyloid Precursor Protein in Neuroblastoma Cells  

Leem, Jae-Yoon (College of Pharmacy, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.1, 2010 , pp. 91-95 More about this Journal
Abstract
Alzheimer's disease (AD) is characterized pathologically by the presence of intracellular neurofibrillary tangles and deposition of ${\beta}$-amyloid (A${\beta}$) peptides. It is urgent to develop effective therapies for the treatment of AD, since our society rapidly accelerate aging. A${\beta}$ peptides have been believed to be neurotoxic and now are also considered to have affects on the mechanism of memory formation, which are generated by processing of amyloid precursor protein (APP). In this study, effects of Styrax Liquides (SL) on the metabolism of APP were analyzed. SL inhibited the secretion of A${\beta}$ from the Neuro2a cell line (APPswe cell) expressing a mutation of APPswe. Immunoblotting study showed that it inhibited ${\beta}$-site APP cleaving enzyme (BACE) from the APPswe cells. We suggest that SL inhibits APP metabolism and A${\beta}$ generation by the means of BACE inhibitory mechanism. This is the first report that SL inhibits the secretion of A${\beta}$ peptides from neuroblastoma cells.
Keywords
alzheimer's disease; ${\beta}$-amyloid peptides; styrax liquides; APPswe; BACE;
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1 Sugimoto, H. Donepezil hydrochloride. a treatment drug for Alzheimer's disease. Chem. Rec. 1: 63-73, 2001.   DOI   ScienceOn
2 Strittmatter, W.J., Weisgraber, K.H., Huang, D.Y., Dong, L.M., Salvesen, G.S., Pericak-Vance, M., Schmechel, D., Saunders, A.M., Goldgaber, D., Roses, A.D. Binding of human apolipoprotein E to synthetic amyloid beta peptide isoform-specific effects and implications for late-onset Alzheimer disease. Proc. Natl. Acad. Sci. USA, 90: 8098-8102, 1993.   DOI   ScienceOn
3 Yu, G., Nishimura, M., Arawaka, S., Levitan, D., Zhang, L., Tandon, A., Song, Y.Q., Rogaeva, E., Chen, F., Kawarai, T., Supala, A., Levesque, L., Yu, H., Yang, D.S., Holmes, E., Milman, P., Liang, Y., Zhang, D.M., Xu, D.H., Sato, C., Rogaev, E., Smith, M., Janus, C., Zhang, Y., Aebersold, R., Farrer, L.S., Sorbi, S., Bruni, A., Fraser, P., St. George-Hyslop, P. Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. Nature 407: 48-54, 2000.   DOI   ScienceOn
4 Wang, R., Sweeney, D., Gandy, S.E. and Sissodia, S.S. The profile of soluble amyloid $\beta$ protein in cultured cell media. J. Biol. Chem. 271: 31894-31902, 1996.   DOI   ScienceOn
5 Sambamurti, K., Hardy, J., Refolo L.M. and Lahiri, D.K. Targeting APP metabolism for the treatment of Alzheimer's disease. Drug Dev. Res. 56: 211-227, 2002.   DOI   ScienceOn
6 Takahashi, Y., Hayashi, I., Tominari, Y., Rikimaru, K., Morohashi, Y., Kan, T., Natsugari, H., Fukuyama, T., Tomita, T. and Iwatsubo, T. Sulindac sulfide is a noncompetitive gamma-secretase inhibitor that preferentially reduces Abeta 42 generation. J. Biol. Chem. 278: 18664-18670, 2003.   DOI   ScienceOn
7 Citron, M., Westaway, D., Xia, W., Carlson, G., Diehl, T., Levesque, G., Johnson-Wood, K., Lee, M., Seubert, P., Davis, A., Kholodenko, D., Motter, R., Sherrington, R., Perry, B., Yao, H., Strome, R., Lieberburg, I., Rommens, J., Kim, S., Schenk, D., Fraser, P., St George Hyslop, P. and Selkoe. D.J. Mutant presenilins of Alzheimer's disease increase production of 42-residue amyloid beta-protein in both transfected cells and transgenic mice. Nat. Med. 3: 67-72, 1997.   DOI   ScienceOn
8 Leem, J.Y., Saura, C.A., Pietrzik, C., Christianson, J., Wanamaker, C., King, L.T., Veselits, M.L., Tomita, T., Gasparini, L., Iwatsubo, T., Xu, H., Green, W.N., Koo, E.H., Thinakaran, G. A role for presenilin 1 in regulating the delivery of amyloid precursor protein to the cell surface. Neurobiol. Dis. 11: 64-82, 2002.   DOI   ScienceOn
9 Skovronsky, D.M., Moore, D.B., Milla, M.E., Doms, R.W., Lee, V.M. Protein kinase C-dependent alpha-secretase competes with beta-secretase for cleavage of amyloid-beta precursor protein in the trans-golgi network. J. Biol. Chem. 275: 2568-2575, 2000.   DOI   ScienceOn
10 Nunan, J., Small, D.H. Regulation of APP cleavage by alpha-, beta- and gamma-secretases. FEBS Lett. 483: 6-10, 2000.   DOI   ScienceOn
11 Kuo, Y.M., Emmerling, M.R., Vigo-Pelfrey, C., Kasunic, T.C., Kirkpatrick, J.B., Murdoch, G.H., Ball, M.J., Roher, A.E. Water-soluble Abeta (N-40, N-42) oligomers in normal and Alzheimer disease brains. J. Biol. Chem. 271: 4077-4081, 1996.   DOI
12 Hardy, J. and Selkoe, D.J. The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics. Science 297: 353-356, 2002.   DOI   ScienceOn
13 Fu, H., Dou, J., Li, W., Cui, W., Mak, S., Hu, Q., Luo, J., Lam, C.S., Pang, Y., Youdim, M.B. and Han, Y. Promising multifunctional anti-Alzheimer's dimer bis(7)-Cognitin acting as an activator of protein kinase C regulates activities of alpha-secretase and BACE-1 concurrently. Eur. J. Pharmacol. 623(1-3):14-21, 2009.   DOI   ScienceOn
14 Sagdic, O., Ozkan, G., Ozcan, M., Ozcelik, S. A study on inhibitory effects of Sigla tree (Liquidambar orientalis Mill. var. orientalis) storax against several bacteria. Phytother. Res. 6: 549-551, 2005.
15 Tung, J.S., Davis, D.L., Anderson, J.P., Walker, D.E., Mamo, S., Jewett, N., Hom, R.K., Sinha, S., Thorsett, E.D., John, V. Design of substrate-based inhibitors of human beta-secretase. J. Med. Chem. 45: 259-262, 2002.   DOI   ScienceOn
16 Lee, S.F., Shah, S., Li, H., Yu, C., Han, W., Yu, G. Mammalian APH-1 interacts with presenilin and nicastrin and is required for intramembrane proteolysis of amyloid-beta precursor protein and Notch. J. Biol. Chem. 277: 45013-45019, 2002.   DOI   ScienceOn
17 Steiner, H., Winkler, E., Edbauer, D., Prokop, S., Basset, G., Yamasaki, A., Kostka, M. and Haass, C. PEN-2 is an integral component of the $\gamma$-secretase complex required for coordinated expression of presenilin and nicastrin. J. Biol. Chem. 277: 39062-39065, 2002.   DOI   ScienceOn
18 Vassar, R., Bennett, B.D., Babu-Khan, S., Kahn, S., Mendiaz, E.A., Denis, P., Teplow, D.B., Ross, S., Amarante, P., Loeloff, R., Luo, Y., Fisher, S., Fuller, J., Edenson, S., Lile, J., Jarosinski, M.A., Biere, A.L., Curran, E., Burgess, T., Louis, J.C., Collins, F., Treanor, J., Rogers, G., Citron, M. $\beta$-Secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 286: 735-741, 1999.   DOI   ScienceOn
19 Cai, H., Wang, Y., McCarthy, D., Wen, H., Borchelt, D.R., Price, D.L., Wong, P.C. BACE1 is the major $\beta$-secretase for generation of A$\beta$ peptides by neurons. Nature Neurosci. 4: 233-234, 2001.   DOI   ScienceOn
20 Corder, E.H., Saunders, A.M., Strittmatter, W.J., Schmechel, D.E., Gaskell, P.C., Small, G.W., Roses, A.D., Haines, J.L., Pericak-Vance, M.A. Gene dose of apolipoprotein E type 4 allele and the risk of Alzheimer's disease in late onset families. Science 261: 921-923, 1993.   DOI
21 Jann, M.W. Rivastigmine, a new-generation cholinesterase inhibitor for the treatment of Alzheimer's disease. Pharmacotherapy 20: 1-12, 2000.   DOI   ScienceOn
22 Selkoe, D.J. Translating cell biology into therapeutic advances in Alzheimer's disease. Nature 399: 23-31 1999.   DOI   ScienceOn
23 Vellas, B. and Fitten, L.J. Research and practice in Alzheimer's disease. Springer Publishing Co. 2000.
24 Zarotsky, V., Sramek, J.J. and Cutler, N.R. Galantamine hydrobromide. an agent for Alzheimer's disease. Am. J. Health Syst. Pharm. 60: 446-452, 2003.
25 Iqbal, K., Sisodia, S.S. and Winblad, B. Alzheimer's disease. Advances in etionlogy, pathogenesis and therapeutics. John Wiley & Sons, Ltd. 2001.
26 Morris, J.C. Classification of dementia and Alzheimer's disease. Acta Neurol. Scand. 165: 41-50, 1996.
27 Braak, H., Braak, E. and Strothjohann, M. Abnormally phosphorylated tau protein related to the formation of neurofibrillary tangles and neuropil threads in the cerebral cortex of sheep and goat. Neurosci Lett. 171: 1-4, 1994.   DOI   ScienceOn
28 Kang, J., Lemaire, H.G., Unterbeck, A., Salbaum, J.M., Masters, C.L., Grzeschik, K.H., Multhaup, G., Beyreuther, K., Muller-Hill, B. The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature 325: 733-736, 1987.   DOI   ScienceOn
29 Selkoe, D.J. The cell biology of beta-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 8: 447-453, 1998.   DOI   ScienceOn