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Neuroprotective Effects of Rehmanniae Radix on PC12 Cells and Hippocampal Neural Cells  

Jo, Jae-Heun (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University)
Shin, Jung-Won (Department of Anatomy & Neurobiology, School of Medicine, Washington University in St.)
Shim, Eun-Shep (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University)
Kim, Bum-Hoi (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University)
Sohn, Young-Joo (Department of Gynecology, College of Oriental Medicine, Sangji University)
Jung, Hyuk-Sang (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University)
Sohn, Nak-Won (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.6, 2007 , pp. 1401-1406 More about this Journal
Abstract
The present study investigated neuroprotective effects Rehmanniae Radix on PC12 cells and hippocampal neural cells. PC12 cells were damage by $H_2O_2$ and nitric oxide and organotypic hippocampal slice cultures were damaged by oxygen-glucose deprivation. Then methanol extract of Rehmanniae Radix was treated with 0.5, 5, and $50\;{\mu}g/ml$ in culture media. Effects of Rehmanniae Radix were evaluated with cell viability assay, PI-staining, and TUNEL-labeling. Treatment of Rehmanniae Radix ($with\;5\;and\;50\;{\mu}g/ml$) produced significant increase of cell viability of PC12 cells damaged by $H_2O_2$ and by SNP-induced nitric oxide. Treatment of Rehmanniae Radix produced significant decrease of PI-uptake % in CA1 ($with\;5\;and\;50\;{\mu}g/ml$) and DG ($with\;50\;{\mu}g/ml$) regions of organotypic hippocampal slice cultures damaged by oxygen-glucose deprivation. Moreover, treatment of Rehmanniae Radix produced significant decrease of TUNEL- positive cells in CA1 ($with\;5\;and\;50\;{\mu}g/ml$) and DG ($with\;50\;{\mu}g/ml$) regions of organotypic hippocampal slice cultures damaged by oxygen-glucose deprivation. These results suggest that methanol extract of Rehmanniae Radix has neuroprotective effects on PC12 cells damaged by oxidative stress and on organotypic hippocampal slice cultures damaged by oxygen-glucose deprivation.
Keywords
neuroprotection; Rehmanniae Radix; PC12 cells; hippocampus; organotypic culture;
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1 김호철. 한약약리학. 서울, 집문당. pp 467-469, 2001
2 이상인, 안덕균, 신민교. 한약임상응용. 서울, 성보사. pp 105-106, 1990
3 황영명, 고병희, 송일병. 생지황, 건지황, 숙지황이 세포성면 역반응 및 체액성면역반응에 미치는 영향. 경희한의대논문집 10: 207-218, 1987
4 이호섭, 류도곤, 윤용갑, 유윤조. 지황 전탕액 투여가 백서의 혈장 Renin 활성도, 혈장 Aldosterone 및 Atrial Natriuretic Peptide 농도에 미치는 영향. 대한한의학회지 17(1):329-335, 1996
5 Li, D.Q., Bao, Y.M., Zhao, J.J., Liu, C.P., Liu, Y., An, L.J. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response, therapeutic time-window and long-term efficacy. Brain Res. 1029(2):179-185, 2004   DOI   ScienceOn
6 Bonde, C., Noraberg, J., Zimmer, J. Nuclear shrinkage and other markers of neuronal cell death after oxygen-glucose deprivation in rat hippocampal slice cultures. Neurosci. Lett. 327: 49-52, 2002   DOI   ScienceOn
7 Monette, R., Small, D.L., Mealing, G., Morley, P. A fluorescence confocal assay to asses neuronal viability in brain slices. Brain Res. Protoc. 2:99-108, 1998   DOI   ScienceOn
8 D aw son, V .L., D aw son, T .M . N itric oxid e actions in neurochemistry. Neurochem. Int. 29: 97-110, 1996   DOI   ScienceOn
9 Rief, D.W., Simmons, R.D. Nitric oxide mediates iron release from ferritin. Arch. Biochem. Biophys. 283: 537-541, 1990   DOI   ScienceOn
10 Dai, Y., But, P.P., Chan, Y.P., Matsuda, H., Kubo, M. Antipruritic and antiinflammatory effects of aqueous extract from Si-Wu-Tang. Biol. Pharm. Bull. 25(9):1175-1178, 2002   DOI   ScienceOn
11 권영달, 송용선. 생지황(生地黃)의 투여가 마우스의 선천면역 및 적응면역 반응에 미치는 영향. 한방재활의학과학회지 8(1):233-254, 1998
12 이시진. 본초강목. 서울, 고문사. p 1205, 1975
13 박정배, 김경식. 지황 수침이 실험적 신성 고혈압 백서의 신장기능에 미치는 영향. 대한침구학회지 11(1):225-239, 1994
14 Li, D.Q., Duan, Y.L., Bao, Y.M., Liu, C.P., Liu, Y., An, L.J. Neuroprotection of catalpol in transient global ischemia in gerbils. Neurosci. Res. 50(2):169-177, 2004   DOI   ScienceOn
15 Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Meth. 65: 55-63, 1983   DOI
16 Hilton, D.A., Love, S., Barber, R. Demonstration of apoptotic cells in tissue sections by in situ hybridization using digoxigenin-labeled poly(A) oligonucleotide probes to detect thymidine-rich DNA sequences. J. Histochem. Cytochem. 45: 13-20, 1997   DOI   ScienceOn
17 Gahwiler, B.H., Capogna, M., Debanne, D., McKinney, R.A., Thompson, S.M. Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20: 471-477, 1997   DOI   ScienceOn
18 Kubo, M., Asano, T., Matsuda, H., Yutani, S., Honda, S. Studies on Rehmanniae radix. III. The relation between changes of constituents and improvable effects on hemorheology with the processing of roots of Rehmannia glutinosa. Yakugaku Zasshi, 116(2):158-168, 1996   DOI
19 권남원, 송병기, 구본홍. 생지황 및 대계가 생쥐의 혈장 Prothrombin Time에 미치는 영향. 경희한의대논문집 5: 259-268, 1982
20 Garthwaite, J., Boultom, C.L. Nitric oxide signaling in the central nervous system. Annu. Rev. Physiol. 57: 683-686, 1995   DOI   ScienceOn
21 Kubo, M., Asano, T., Shiomoto, H., Matsuda, H. Studies on rehmanniae radix. I. Effect of 50% ethanolic extract from steamed and dried rehmanniae radix on hemorheology in arthritic and thrombosic rats. Biol. Pharm. Bull. 17(9):1282-1286, 1994   DOI   ScienceOn
22 Trembovler, V., Abu-Raya, S., Shohami, E. Synergism between tumor necrosis factor-alpha and $H_2O_2$ enhances cell damage in rat PC12 cells. Neurosci. Lett. 353(2):115-118, 2003   DOI   ScienceOn
23 Jiang, B., Liu, J.H., Bao, Y.M., An, L.J. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase cascade. Toxicon. 43(1):53-59, 2004   DOI   ScienceOn
24 Zimmer, J., Gahwiler, B.H. Cellular and connective organization of slice cultures of the rat hippocampus and fascia dentata. J. Comp. Neurol. 228: 432-446, 1984   DOI   ScienceOn
25 Youdim, M.B.H., Ben-Shachar, D., Eshel, G., Finterg, J.P.M., Risderer, P. The neurotoxicity of iron and nitric oxide. Relevance to the etiology of Parkinson's disease. Adv. Neurol. 60: 259-266, 1993
26 Brana, C., Benham, C., Sundstrom, L. A method for characterising cell death in vitro by combining propidium iodide staining with immunohistochemistry. Brain Res. Protoc. 10: 109-114, 2002   DOI   ScienceOn
27 이상인. 본초학. 서울, 의약사. p 105, 1975
28 Lee, H.S., Kim, S.T., Cho, D.K. Effects of rehmanniae radix water extract on renal function and renin secretion rate in unanesthetized rabbits. Am. J. Chin. Med. 21(2):179-186, 1993   DOI   ScienceOn
29 Xu, G.P., Dave, K.R., Vivero, R., Schmidt-Kastner, R., Sick, T.J., Porez-Pinzon M.A. Improvement in neuronal survival after ischemic preconditioning in hippocampal slice cultures. Brain Res. 952: 153-158, 2002   DOI   ScienceOn
30 Desolea, M.S., Sciolab, L., Sircanaa, S., Godania, C., Mighelia, R., Delogua, M.R., Pirasa, G., De Natalea, G., Mielea, E. Protective effect of deferoxamine on sodium nitroprusside induced apoptosis in PC12 cells. Neurosci. Lett. 247: 1-4, 1998   DOI   ScienceOn
31 Gavrieli, Y., Sherman, Y., Ben-Sasson, S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119: 493-501, 1992   DOI   ScienceOn