Browse > Article

Cheonggi-san Inhibits Atopy Dermatitis in NC/Nga Mouse through Regulation of iNOS mRNA Expresssion & NO production  

Ahn, Sang-Hyun (Department of Anatomy, Oriental Medicine College, Dongguk University)
Kim, Ho-Hyun (Department of Physiology, Oriental Medicine College, Semyung University)
Kim, Jin-Taek (Department of Anatomy, Oriental Medicine College, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.5, 2007 , pp. 1092-1098 More about this Journal
Abstract
Inducible nitric oxide synthase (iNOS) are important inflammation enzyme and severe up-nitric oxide (NO) production by this enzyme has been intricate with pathogenesis of atopy dermatitis. The present study was designed in order to determine whether Cheonggi-san could inhibit atopy dermatitis through modulation of iNOS mRNA expression and NO production. We found that iNOS mRNA expression and NO production in RAW 264.7 macrophages stimulated with lipopolysaccharide dose-dependantly decreased by Cheonggi-san extract treatment (0.5 - 2.0 mg/ml). The distribution of iNOS positive reacted cell in NC/Nga mice with atopy dermatitis were decreased by Cheonggi-san extract treatment (2.5 ml/kg/day) and apoptosis were increased. These data likely indicate that Cheonggi-san may act as inflammatory regulator for atopy dermatitis and may be possible to develop useful agent for chemoprevention of NO intricate inflammatory diseases.
Keywords
Cheonggi-san; Atopy dermatitis; iNOS; NO; NC/Nga mice;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Guzik, T.J., West, N.E., Black, E., McDonald, D., Ratnatunga, C., Pillai, R., Channon, K.M. Vascular superoxide production by NAD(P)H oxidase: association with endothelial dysfunction and clinical risk factors. Circ Res. 86(9):E85-90, 2000   DOI   ScienceOn
2 Hibbs, J.B. Jr, Taintor, R.R., Vavrin, Z. Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrite. Science 235(4787):473-476, 1987   DOI
3 丁光迪. 諸病源候論校注. 北京, 人民衛生出版社, pp 1411- 1412, 1994
4 吳謙. 醫宗金監. 北京, 人民衛生出版社, p 443, 1982
5 이종대. 새로 보는 빈용 202처방. 서울, 도서출판 정담, p 758, 2005
6 Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Yun, Y.G., Kwon, T.O., Chung, H.T. Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide. Gen Pharmacol. 35: 21-28, 2000   DOI   ScienceOn
7 Lee, B.G., Kim, S.H., Zee, O.P., Lee, K.R., Lee, H.Y., Han, J.W., Lee, H.W. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophagesby two-carboline alkaloids extractes from Melia azedarach. Eur. J. Pharmacol. 406: 301-309, 2000   DOI   ScienceOn
8 이동언, 이재령, 김영우, 권영규, 변성희, 신상우, 서성일, 권택규, 변준석, 김상찬. 금은화 및 금은화전초가 RAW 264.7 cell에서 LPS로 유도된 NO의 생성, iNOS, COX-2 및 cytokine에 미치는 영향. 동의생리병리학회지 19(2):481-489, 2005
9 Tan, C.M., Xenoyannis, S., Feldman, R.D. Oxidant stress enhances adenylyl cyclase activation. Circ Res. 77(4):710- 717, 1995   DOI   ScienceOn
10 Nussler, A.K., Billiar, T.R. Inflammation, immunoregulation, and inducible nitric oxide synthase. J Leukoc Biol. 54(2):171-178, 1993   DOI
11 Stuehr, D.J., Marletta, M.A. Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. Proc Natl Acad Sci USA. 82(22):c, 1985
12 Belvisi, M., Barnes, P.J., Larkin, S., Yacoub, M., Tadjkarimi, S., Williams, T.J., Mitchell, J.A. Nitric oxide synthase activity is elevated in inflammatory lung disease in humans. Eur J Pharmacol. 283(1-3):255-258, 1995   DOI
13 허준. 對譯東醫寶鑑. 서울, 법인문화사, p 732, 1999
14 윤용갑. 동의방제와 처방해설. 서울, 의성당, pp 583-586, 1998
15 王冰 編撰. 新編黃帝內經素問. 서울, 대성문화사, p 60, 105, 327, 1994
16 Christophers, E., Mrowietz, U. The inflammatory infiltrate in psoriasis. Clin. Dermatol. 13: 131-135, 1995   DOI   ScienceOn
17 Halbert, A.R., Weston, W.L., Morelli, J.G. Atopic dermatitis: is it an allergic disease? J Am Acad Dermatol. 33(6):1008-1018, 1995   DOI   ScienceOn
18 Motta, S., Monti, M., Sesana, S., Mellesi, L., Ghidoni, R., Caputo, R. Abnormality of water barrier function in psoriasis. Arch Dermatol. 130: 452-456, 1994   DOI   ScienceOn
19 Hunnum, Y.A., Bell, R.M. Function of sphingolipid breakdown productionin cellular regulation. Science. 243: 500-507, 1989   DOI
20 Minehiro, O., Takashi, Y., Hideoki, O. Detergent-induced epidermal barrier dysfunction and its prevention. J. Dermatol. Sci. 30: 173-179, 2002   DOI   ScienceOn
21 Wu, M.X., Ao, Z., Prasad, K.V., Wu, R., Schlossman, S.F. IEX-1L, an apoptosis inhibitor involved in NF-kappaB-mediated cell survival. Science. 281(5379):998-1001, 1998   DOI
22 Lowenstein, C.J., Alley, E.W., Raval, P., Snowman, A.M., Snyder, S.H., Russell, S.W., Murphy, W.J. Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide. Proc Natl Acad Sci USA. 90(20):9730-9734, 1993
23 Xie, Q.W., Kashiwabara, Y., Nathan, C. Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. J Biol Chem. 269(7):4705-4708, 1994
24 Min-Hsiung Pan, Ching-Shu Lai, Ying-Jan Wang, Chi-Tang Ho. Acacetin suppressed LPS-induced up-expression of iNOS and COX-2 in murine macrophages and TRA-induced tumor promotion in mice. Biochem Pharmacol 72: 1293-1303, 2006   DOI   ScienceOn
25 황도연. 對譯證脈.方藥合編. 서울, 남산당, p 256, 257, 2002
26 Ai-Hsiang LO, Yu-Chih Liang, Shoei-Yn Lin-Shiau, Chi-Tang Ho, Jen-Kun Lin. Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating NF-${\kappa}B$ in mouse macrophages. Carcinogenesis 23(6):983-991, 2002   DOI   ScienceOn
27 Radi, R., Beckman, J.S., Bush, K.M., Freeman, B.A. Peroxynitrite- induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys. 288(2):481-487, 1991   DOI   ScienceOn
28 김영신. 淸肌散 및 淸肌散加味方의 항알레르기와 면역반응에 대한 실험적 연구. 경희대학교 박사논문, 1990
29 Eiserich, J.P., Baldus, S., Brennan, M.L., Ma, W., Zhang, C., Tousson, A., Castro, L., Lusis, A.J., Nauseef, W.M., White, C.R., Freeman, B.A. Myeloperoxidase, a leukocyte-derived vascular NO oxidase. Science. 296(5577):2391-2394, 2002   DOI
30 Lim, D.G., Sweeney, S., Bloodsworth, A., White, C.R., Chumley, P.H., Krishna, N.R., Schopfer, F., O'Donnell, V.B., Eiserich, J.P., Freeman, B.A. Nitrolinoleate, a nitric oxide-derived mediator of cell function: synthesis, characterization, and vasomotor activity. Proc Natl Acad Sci USA. 99(25):15941-15946, 2002
31 Lee, H.S., Kim, S.K., Han, J.B., Choi, H.M., Park, J.H., Kim, E.C., Choi, M.S., An, H.J., Um, J.Y., Kim, H.M., Min, B.I. Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice. Br J Dermatol. 155(1):33-38, 2006   DOI   ScienceOn
32 Min-Hsiung Pan, Shoei-Yn Lin-shiau, Jen-Kun Lin. Comparative Studies on the suppression of nitric oxide synthase by curcumin and Its Hydrogenated metabolites through down-regulation of I$\kappa$B kinase and NF-${\kappa}B$ activation in macrophages. Biochem Pharmacol 60: 1665-1676, 2000   DOI   ScienceOn
33 강명길. 濟衆新編. 서울, 행림서원, p 150, 151, 1971
34 Baeuerle, P.A., Baltimore, D. NF-$\kappa$B - Ten years after. Cell. 87: 13-20, 1996   DOI   ScienceOn
35 Beckman, J.S., Beckman, T.W., Chen, J., Marshall, P.A., Freeman, B.A. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA. 87(4):1620-1624. 1990
36 Panza, J.A., Quyyumi, A.A., Brush, J.E. Jr, Epstein, S.E. Abnormal endothelium-dependent vascular relaxation in patients with essential hypertension. N Engl J Med. 323(1):22-27, 1990   DOI   ScienceOn
37 Muller, M.J., Vollmar, B., Friedl, H.P., Menger, M.D. Xanthine oxidase and superoxide radicals in portal triad crossclamping-induced microvascular reperfusion injury of the liver. Free Radic Biol Med. 21(2):189-197, 1996   DOI   ScienceOn
38 危亦林. 世醫得效方. 上海, 上海科學技術出版社, p 962, 1997
39 Baeuerle, P.A. I$\kappa$B-NF-$\kappa$B structure: at the interface of inflammation control. Cell. 95: 729-731, 1998   DOI   ScienceOn
40 차관배, 김윤식, 설인찬. 아토피피부염에 관한 문헌적 고찰. 대전대학교 한의학연구소 논문집, 14(2):113-126, 2005
41 Paige, D.G., Morse-Fisher, N., Harper, J.I. Quantification of stratum corneum ceramides and lipid envelope ceramide in the hereditary ichthyoses. Br. J. dermatol. 131: 23-27, 1994   DOI   ScienceOn
42 Andrea, H., Thomas, K., Josef, P., Konrad, S. Physiology and pathophysiology of sphingolipid metabolism and signaling. Biochemica et Biophysica Acta. 1485: 63-99, 2000   DOI
43 변성희, 양재하, 김상찬. 현삼메탄올 추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 및 nitric oxide 생성에 미치는 영향. 대한본초학회지 20(2):7-16, 2005
44 Radi, R., Beckman, J.S., Bush, K.M., Freeman, B.A. Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J Biol Chem. 266(7):4244-4250, 1991
45 Elias, P.M., Williams, M.L., Maloney, M.E., Bonifas, J.A., Brown, B.E., Grayson, S., Epstein, E.H. Straum corneum lipids in disorders of cornification. J. Clin. Invest. 74: 1414-1421, 1984   DOI
46 Floyd, R.A. Neuroinflammatory processes are important in neurodegenerative diseases: an hypothesis to explain the increased formation of reactive oxygen and nitrogen species as major factors involved in neurodegenerative disease development. Free Radic Biol Med. 26(9-10):1346-1355, 1999   DOI   ScienceOn
47 박민철, 김진만, 홍철희, 황충연. 아토피피부염의 동서의학적 문헌 고찰. 대한안이비인후피부과학회지 15(1):226-252, 2002
48 박은정, 김양귀. 淸肌散과 加減淸肌散이 마우스의 항알레르기 및 면역반응에 미치는 영향. 대한한방소아과학회지 12(1): 183-210, 1998
49 陳實功. 外科正宗. 北京, 中醫古籍出版社, p 242, 261, 1999
50 채병윤. 한방외과학. 서울, 고문사, p 311, 312, 407, 1972
51 Takano, H., Yanagisawa, R., Inoue, K., Ichinose, T., Sadakane, K., Yoshikawa, T. Di-(2-ethylhexyl) phthalate enhances atopic dermatitis-like skin lesions in mice. Environ Health Perspect. 114(8):1266-1269, 2006   DOI   ScienceOn
52 전국한의과대학 본초학교수. 본초학. 서울, 도서출판 영림사, p 127, 128,131, 136, 142, 149, 155, 260, 302, 351, 409, 458, 460, 504, 531, 540, 2000
53 Anggard, E. Nitric oxide: mediator, murderer, and medicine. Lancet. 9: 1199-1206, 1994
54 Kojda, G., Harrison, D. Interactions between NO and reactive oxygen species: pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc Res. 43(3):562-571, 1999   DOI   ScienceOn
55 Villa, L.M., Salas, E., Darley-Usmar, V.M., Radomski, M.W., Moncada, S. Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart. Proc Natl Acad Sci USA. 91(26):12383-12387, 1994
56 Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Kwon, T.O., Yun, Y.G., Kim, N.Y., Chung, H.T. Inhibitory effects of methanol extracts of Cyrerus rotundus rhizomes on nitric oxide and superoxide production by murine macrophages cell line, RAW 264.7 cells. J Ethnopharmacol. 76: 59-64, 2001   DOI   ScienceOn
57 김혜정, 채병윤. 淸肌散의 효능에 관한 실험적 연구. 대한한방외관과학회지 3(1):25-39, 1990