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Verification of Cytotoxicity Against Cancer Cell Line and Estrogen-like Activity of Cheongkukjang  

Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.1, 2007 , pp. 153-157 More about this Journal
Abstract
In order to evaluate the cancer preventive and estrogenic compounds in soybean and Cheongkukjang, MTT assay and in vitro test system for the evaluation of the estrogenic activity were applied. The fractions from the ethanol extract of soybean and Cheongkukjang were prepared by the systematic extraction procedure with the solvents such as hexane, ethyl ether, butanol, methanol and H$_2$O. Ethyl ether fractions of soybean and Cheongkukjang showed the highest cytotoxicity against U937 cell line in dose dependent manner, and ethyl ether fraction of Cheongkukjang showed two times higher cytotoxicity than that of soybean. Aqueous fraction of soybean and ethyl ether fraction of Cheongkukjang revealed the highest estrogenic activity and activity was higher in the fractions of Cheongkukjang than soybean. Mixture of Spirulina and Cheongkukjang showed synergistic activity. These observations concerning cancer preventive and estrogen effects of soybean and Cheongkukjang suggest that these materials possess useful ingredients for the prevention of cancer and/or postmenopausal disorder.
Keywords
Cheongkukjang; MTT; estrogenic activity; soybean; U937;
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Times Cited By KSCI : 4  (Citation Analysis)
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