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Study on Apoptosis-Inducing Effect and Mechanism by Tarisodokyeum in PC-3 cells  

Park, Hyung-Kwon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim, Eun-Kyung (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Han, Mi-Jeong (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Song, Mi-Young (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Lee, Young-Rae (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Park, Byung-Hyun (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 946-950 More about this Journal
Abstract
Takrisodokyeum (TRSDY) has been known to exert anti-tumoral activity in Korea. However, its molecular mechanism of action is not understood. In this study, we found that TRSDY induced apoptosis in androgen-independent prostate cancer PC-3 cells as evidenced by DNA fragmentation. Our data demonstrated that TRSDY-induced apoptotic cell death was accompanied by decreases of PAKT and $NF-{\kappa}$ activation, which is resulted from inhibition of $I{\kappa}B-{\alpha}$ degradation. But TRSDY-induced apoptotic effect of PC-3 cells was independent of Par-4 expression. Taken together, these results suggest that TRSDY inhibits AKT phosphorylation and $NF-{\kappa}B$ activation, and eventually leads to apoptotic cell death in androgen independent prostate cancer PC-3 cells.
Keywords
Takrisodokyeum; PC-3 cell; apoptosis; p-AKT; $NF-{\kappa}B$; $I{\kappa}B-{\alpha}$;
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