Browse > Article

Effects of Water Extracts from Chaenomeles sinensis, Polygonum cuspidatum and Boswellia carterii on LPS-Induced MMP-9 Activation in Raw 264.7 Cell  

Lee Tae-Jin (Department of Immunology, College of Medicine, Keimyung University)
Kim Yeoun-Hee (Department of Immunology, College of Medicine, Keimyung University)
Shu Seong-Il (Department of Microbiology, College of Medicine, Keimyung University)
Shin Sang-Woo (College of Oriental Medicine, Daegu Haay University)
Kim Sang-Chan (College of Oriental Medicine, Daegu Haay University)
Kwon Young-Kyu (College of Oriental Medicine, Daegu Haay University)
Park Jong-Wook (Department of Immunology, College of Medicine, Keimyung University)
Kwon Taeg-Kyu (Department of Immunology, College of Medicine, Keimyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.1, 2006 , pp. 37-42 More about this Journal
Abstract
Matrix metalloproteinase-9 (MMP-9) is considered to be an important component in the progression of inflammation. Monocytes/macrophages are prominent at inflammation sites, and activation of these cells by stimulants such as lipopolysaccharide (LPS) leads to the production of significant amounts of MMP-9. Here, we show that LPS-induced MMP-9 production and activation was inhibited by the water extract from the fruit of Chaenomeles sinensis (CS), the root of Polygonum cuspidatum (PC), but increased by the extract from Boswellia carterii (BC). To investigate the mechanism by which those extracts inhibits MMP-9 activation, we examined the level of MMP-9 mRNA expression. We observed a significant change in the MMP-9 expression between LPS alone and LPS plus Chaenomeles sinensis and Polygonum cuspidatum extracts-treated cells. In addition, LPS significantly up-regulated MMP-9 promoter activity in Raw 264.7 cells, which was attenuated by the CS and PS extracts. However, water extracts from Boswellia carterii increased MMP-9 expression and MMP-9 promoter activity which were induced by LPS treatment in Raw 264.7 cells. These data suggest that water extracts from Chaenomeles sinensis and Polygonum cuspidatum can modulate anti-inflammatory immune response, which may be in part associated with the regulation of MMP-9 production and/or activation through the regulation of MMP-9 expression in mouse macrophage cells.
Keywords
Chaenomeles sinensis; Polygonum cuspidatum; Boswellia carterii; MMP-9;
Citations & Related Records
연도 인용수 순위
  • Reference
1 MacMicking, J.D., Nathan, C., Hom, G., Chartrain, N., Fletcher, D.S., Trumbauer, M., Stevens, K., Xie, Q.W., Sokol, K., Hutchinson, N., et al. Altered responses to bacterial infection and endotoxic shock in mice lacking inducible nitric oxide synthase. Cell. 81(4):641-650, 1995   DOI   ScienceOn
2 Liu, R.H., Hotchkiss, J.H. Potential genotoxicity of chronically elevated nitric oxide: a review. Mutat Res. 339(2):73-89, 1995   DOI   ScienceOn
3 Visse, R., Nagase, H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res. 92(8):827-839, 2003   DOI   ScienceOn
4 Douglas, D.A., Shi, Y.E., Sang, Q.A. Computational sequence analysis of the tissue inhibitor of metalloproteinase family. J Protein Chem. 16(4):237-255, 1997   DOI   ScienceOn
5 Lee, M.H., Han, Y.N. A new in vitro tissue factor inhibitory triterpene from the fruits of Chaenomeles sinensis. Planta Med. 69(4):327-331, 2003   DOI   ScienceOn
6 Takada, Y., Aggarwal, B.B. Betulinic acid suppresses carcinogen-induced NF-kappa B activation through inhibition of I kappa B alpha kinase and p65 phosphorylation: abrogation of cyclooxygenase-2 and matrix metalloprotease-9. J Immunol. 171(6):3278-3286, 2003   DOI
7 Yang, F., Zhang, T., Ito, Y. Large-scale separation of resveratrol, anthraglycoside A and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc by high-speed counter- urrent chromatography. J Chromatogr A. 919(2):443-448, 2001   DOI   ScienceOn
8 Xiao, K., Xuan, L., Xu, Y., Bai, D., Zhong, D. Constituents from Polygonum cuspidatum. Chem Pharm Bull (Tokyo). 50(5):605-608, 2002   DOI   ScienceOn
9 Lu, Y., Wahl, L.M. Production of matrix metalloproteinase-9 by activated human monocytes involves a phosphatidylinositol-3 kinase/Akt/IKKalpha/NF-kappaB pathway. J Leukoc Biol. 78(1):259-265, 2005   DOI   ScienceOn
10 Chen, L., Han, Y., Yang, F., Zhang, T. High-speed counter- current chromatography separation and purification of resveratrol and piceid from Polygonum cuspidatum. J Chromatogr A. 907(1-2):343-346, 2001   DOI
11 Kim, M.S., Park, M.J., Kim, S.J., Lee, C.H., Yoo, H., Shin, S.H., Song, E.S., Lee, S.H. Emodin suppresses hyaluronic acid-induced MMP-9 secretion and invasion of glioma cells. Int J Oncol. 27(3):839-846, 2005
12 Kallakury, B.V., Karikehalli, S., Haholu, A., Sheehan, C.E., Azumi, N., Ross, J.S. Increased expression of matrix metalloproteinases 2 and 9 and tissue inhibitors of metalloproteinases 1 and 2 correlate with poor prognostic variables in renal cell carcinoma. Clin Cancer Res. 7(10):3113-3119, 2001
13 Nikkola, J., Vihinen, P., Vlaykova, T., Hahka-Kemppinen, M., Kahari, V.M., Pyrhonen, S. High expression levels of collagenase-1 and stromelysin-1 correlate with shorter disease-free survival in human metastatic melanoma. Int J Cancer. 97(4):432-438, 2002   DOI   ScienceOn
14 MacMicking, J., Xie, Q.W., Nathan, C. Nitric oxide and macrophage function. Annu Rev Immunol. 15:323-350, 1997   DOI   ScienceOn
15 Cetkovic-Cvrlje, M., Eizirik, D.L. TNF-alpha and IFN-gamma potentiate the deleterious effects of IL-1 beta on mouse pancreatic islets mainly via generation of nitric oxide. Cytokine. 6(4):399-406, 1994
16 Hamauzu, Y., Yasui, H., Inno, T., Kume, C., Omanyuda, M. Phenolic profile, antioxidant property, and anti-influenza viral activity of Chinese quince (Pseudocydonia sinensis Schneid.), quince (Cydonia oblonga Mill.), and apple (Malus domestica Mill.) fruits. J Agric Food Chem. 53(4):928-934, 2005   DOI   ScienceOn
17 Woo, C.H., Lim, J.H., Kim, J.H. Lipopolysaccharide induces matrix metalloproteinase-9 expression via a mitochondrial reactive oxygen species-p38 kinase-activator protein-1 pathway in Raw 264.7 cells. J Immunol. 173(11):6973-6980, 2004   DOI
18 McMillan, J.I., Weeks, R., West, J.W., Bursten, S., Rice, G.C., Lovett, D.H. Pharmacological inhibition of gelatinase B induction and tumor cell invasion. Int J Cancer. 67(4):523-531, 1996   DOI   ScienceOn
19 全國韓醫科大學 本草學敎授. 本草學. 永林社, 서울, pp 272-273, 420-421, 410-411, 2003
20 Cheon, H., Yu, S.J., Yoo, D.H., Chae, I.J., Song, G.G., Sohn, J. Increased expression of pro-inflammatory cytokines and metalloproteinase-1 by TGF-beta1 in synovial fibroblasts from rheumatoid arthritis and normal individuals. Clin Exp Immunol. 127(3):547-552, 2002   DOI   ScienceOn
21 Sato, H., Seiki, M. Regulatory mechanism of 92 kDa type IV collagenase gene expression which is associated with invasiveness of tumor cells. Oncogene, 8:395-405, 1993
22 박래길, 오광록, 이광규, 문연자, 김정훈, 우원홍, 유향추출물의 HL-60 혈액암세포에서 세포사멸 유도효과. 약학회지 45(2):161-168, 2001
23 Parsons, S.L., Watson, S.A., Brown, P.D., Collins, H.M., Steele, R.J. Matrix metalloproteinases. Br J Surg. 84(2):160-166, 1997   DOI   ScienceOn
24 Woo, J.H., Lim, J.H., Kim, Y.H., Suh, S.I., Min do, S., Chang, J.S., Lee, Y.H., Park, J.W., Kwon, T.K. Resveratrol inhibits phorbol myristate acetate-induced matrix metalloproteinase-9 expression by inhibiting JNK and PKC delta signal transduction. Oncogene. 23(10):1845-1853, 2004   DOI   ScienceOn
25 Kishnani, N.S., Tabrizi-Fard, M.A., Fung, H.L. Diethyldithiocarbamate prolongs survival of mice in a lipopolysaccharide-induced endotoxic shock model: evidence for multiple mechanisms. Shock. 11(4):264-268, 1999   DOI   ScienceOn
26 Jayasuriya, H., Koonchanok, N.M., Geahlen, R.L., McLaughlin, J.L., Chang, C.J. Emodin, a protein tyrosine kinase inhibitor from Polygonum cuspidatum. J Nat Prod. 55(5):696-698, 1992   DOI
27 Woo, J.H., Park, J.W., Lee, S.H., Kim, Y.H., Lee, I.K., Gabrielson, E., Lee, S.H., Lee, H.J., Kho, Y.H., Kwon, T.K. Dykellic acid inhibits phorbol myristate acetate-induced matrix metalloproteinase-9 expression by inhibiting nuclear factor kappa B transcriptional activity. Cancer Res. 63(12):3430-3434, 2003
28 Higuchi, M., Higashi, N., Taki, H., Osawa, T. Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. J Immunol. 144(4):1425-1431, 1990
29 Watanabe, H., Nakanishi, I., Yamashita, K., Hayakawa, T., Okada, Y. Matrix metalloproteinase-9 (92 kDa gelatinase/ type IV collagenase) from U937 monoblastoid cells: correlation with cellular invasion. J Cell Sci. 104 (Pt4):991-999, 1993