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Protective Effects of Radix Polygalae on Dopamine-induced Cell Death in Human SH-SY5Y Dopaminergic Neuroblastoma Cells  

Lee Ji Yong (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
Park Jae Hyeon (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
Kim Kyung Yeol (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
Kim Tae Heon (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
Kang Hyung Won (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
Lyu Young Su (Department of Neuropsychiatry, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.2, 2004 , pp. 544-552 More about this Journal
Abstract
In oriental medicine, Radix Polygalae(RP) has been to treat tremors et al. But the mechanism how to decrease tremors was not known. The purpose of this study was to investigate the effect of RP on neurodegenerative disease. We used RP to execute the study of this defense mechanism on dopamine-induced cell death in human SH-SY5Y dopaminergic neuroblastoma cells. MTT assay was used to know the cytotoxicity of dopamine and the defense mechanism. As a result of this experiment, dopamine had cytotoxicity in human SH-SY5Y cells, but when it treated with RP, the cell survival rate increased. This suppressed the cell apoptosis, activation of caspase-3 protease, production of ROS, and repair of membrane potential change. In conclusion, RP has the protective effect on dopamine-induced cell death in human SH-SY5Y dopaminergic neuroblastoma cells, so this could be an effective agent on the neurodegenerative disease like Parkinsonism.
Keywords
SH-SY5Y; Radix Polygalae; Dopamine; Neurodegenerative disease;
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