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Effects of Ginseng Radix Against Osteoblasts Damaged by Endocrine Disrupting Chemicals  

Hong Gi Youn (Department of Obstetric and Gynecology, School of Medicine, Wonkwang University)
Park Seung Taeck (Institution for Medical Science, Wonkwang University)
Jeon Byung Hun (College of Oriental Medicine, Wonkwang University)
Seo Eun A (Medicinal Resources Research Center, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.2, 2002 , pp. 338-342 More about this Journal
Abstract
In order to evaluate the effect of Ginseng Radix(GR) against endocrine disrupting chemicals(EDC), cultured mouse osteoblasts were preincubated with various concentrations of GR extract before the exposure of Bisphenol A for 12 hours. Cytotoxic effect of Bisphenol A was measured by the XTT assay. In addition, the protective effect of GR over Bisphenol A-induced cytotoxicity on osteoblasts was assessed by the DNA and protein synthesis in these cultures. The results were as follows : Osteoblastic cell viability was decreased in dose and time dependent manner after exposed to various concentrations of Bisphenol A. Midcytotoxicity value(MCV50) of Bisphenol A was determined at 6μM Bisphenol A after osteoblasts were grown for 12 hours in the media containing various concentrations of Bisphenol A. Amount of DNA synthesis was increased in dose-dependent manner after cultured osteblasts were pretreated with GR for 2hrs before exposure to Bisphenol A for 12 hours. Amount of protein synthesis was increased in dose-dependent manner after cultured osteoblasts were pretreated with GR for 2 hours before exposure of Bisphenol A for 12 hours. From these results, it is suggested that Bisphenol A was highly toxic by the decrease of the cell viability, and GR is effective in the prevention of Bisphenol A-induced cytotoxicity by the increase of DNA and protein syntheses in cultured mouse osteoblasts.
Keywords
Ginseng Radix(GR); Bisphenol A; Osteoblast;
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